Uso del azul brillante de cresilo en la selección de ovocitos competentes para la producción in vitro de embriones

Abstract

The objective was to evaluate the Brilliant Cresyl Blue (BCB) in the selection of oocytes for the production of embryos in vitro (PIV). Three treatments were evaluated. G1 = Witness without BCB exposure; G2 = staining positive oocytes (BCB +) and G3 = staining negative oocytes (BCB-). Morphometry, maturation (IVM), meiotic configuration, cleavage and embryos were determined. 2,340 cumulus oocyte complexes (COCs) were obtained, classified as suitable and not suitable for IVP. The 2/3 parts were exposed for 90 min. To BCB and the rest incubated for 90 min in H-SOF. 25% of COCs from each group were taken to assess the morphometry. The remaining 75% was placed in maturing medium for 24h. After IVM, 25% of COCs were used for morphometry and meiotic progression. The remaining 50% was in vitro fertilized (IVF) and cultivated. Cleavage was evaluated 48h after IVF and embryos on day 8. 62.3% of G2 oocytes were BCB + and had a larger diameter before (135, 9 μm) and after (134, 8 μm) of IVM. (P <0.05) vs group 3 BCB- (128, 1 and 127, 6 μm) and the Witness (129, 0 and 128, 2 μm). Maturation and meiotic configuration were not different between groups (P> 0.05). Cleavage was higher (P <0.05) in the Control (43.4%) than the BCB + group (33.5%). Finally, the global percentage of embryos was higher in BCB + (52.6%) than the Controlling (32.6%) and BCB- (33.5%). It is concluded that the BCB allowed to select oocytes with a larger diameter, although it did not allow to increase the percentage of maturation and cleavage, it contributed to improving the overall percentage of embryos obtained in vitro.