Person:
Galarza Lucero, Diego Andrés

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Birth Date

1983-06-21

ORCID

0000-0002-0266-5431

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57201315440

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Universidad de Cuenca, Cuenca, Ecuador
Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Laboratorio de Biotecnología de La Reproducción Animal, Cuenca, Ecuador

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Ecuador

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Facultad de Ciencias Agropecuarias
El 21 de abril de 1982, por resolución del Honorable Consejo Universitario se establece la Facultad de Ciencias Agropecuarias, de la Universidad de Cuenca. La Facultad de Ciencias Agropecuarias es una institución formadora de talento humano en el área agronómica, a través de una educación de calidad, centrada en la investigación y vinculación con la colectividad. Los futuros profesionales médicos veterinarios zootecnistas e ingenieros agrónomos, durante su permanencia en las aulas y estudio de campo, desarrollan conocimientos científicos-tecnológicos, competencias y destrezas en procesos de producción agropecuaria. Se los prepara con el fin de preservar la salud de los animales y recursos naturales, fomentando la seguridad alimentaria, respetando el medio ambiente dentro del marco del Buen Vivir, englobado en las necesidades de la región y el país.

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Galarza Lucero

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Diego Andrés

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Now showing 1 - 6 of 6
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    Publication
    Methods of collection, extender type, and freezability of semen collected from creole bulls raised in the tropical highlands of Ecuador
    (2019) Argudo Garzon, Daniel Ernesto; Galarza Lucero, Diego Andrés; Bueno Leon, Hernan Patricio; Iñiguez Gutierrez, Carlos Ulises; Méndez Álvarez, María Silvana; Soria Parra, Manuel Elías; Torres Inga, Carlos Santiago; Perea Ganchou, Fernando Pedro; Alberio Null, Ricardo Horacio
    This study was conducted to determine the best combination between two collection method and two extenders in the cryopres-ervation of semen from creole bulls adapted to highlands of the Ecuadorian Andes. Sixty ejaculates from three adult Creole bulls were evaluated after collection by artificial vagina (AV) and electroejaculation (EE). Semen samples were split into two aliquots and diluted with a soy lecithin extender (Andromed®; A) or an egg yolk-containing extender (Triladyl®; T) and packed in straws of 0.25 ml with 20 × 106 sperms. Optical microscopy and computer-assisted semen analysis system (CASA) were used to evaluate semen quality characteristics. The effects of collection methods and extender type as well as its interaction were evaluated by a factorial ANOVA and Bonferroni’s test. Semen samples collected with EE and frozen with T (EE-T) and A (EE-A) had greater proportion of spermatozoa with optical assessed individual progressive motility (IPM), plasmatic membrane intact (HosT), and lower tail abnormalities than those obtained with AV and frozen with the same extenders (AV-T and AV-A);however, differences were significant only between EE-A and AV-T. CASA assessment indicated that the total mobility (TM) was greater (P < 0.05) in semen samples diluted with T, although these samples had a greater proportion (P < 0.05) of sperms with local motility (LM) and fewer immobile sperms (IS), than those extended with A. Generally, semen samples obtained with EE or AV and diluted with T seems to be the best option to ciopreserve gametes of Creole bulls raised in highlands of Ecuadorian Andes.
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    La suplementación de jalea real a diluyente de base sintética y no sintética ayuda a mantener la motilidad y cinética de esperma de toro refrigerado
    (Universidad de Cuenca, 2022) Galarza Lucero, Diego Andrés; Guanga Tenempaguay, Jacqueline; Lucero Zhumi, Juan Ismael; Samaniego Campoverde, Jorge Xavier; Galarza Lucero, Diego Andrés; Galarza Lucero, Diego Andrés
    Royal Jelly (RJ) is a substantial nutrient produced by the hypopharynx and mandibular glands of worker bees from 4 to 11 days of age, and its composition is mainly based on water (60–70%), protein (27–41% ) and carbohydrates (30%), lipids (8–19%), vitamins (A, B, C, E), salts, and amino acids. Due to its composition, JR has been successfully used in sperm cryopreservation at concentrations from 0.2 to 1% (w/v) supplemented with synthetic base extenders (eg TRIS or TALP). Its effect has been evidenced in the increase of motility, viability, functionality of the plasmatic membrane and fertilizing capacity of fresh and cryopreserved ram sperm. Conventional freezing requires the use of penetrating cryoprotectants (eg glycerol) to prevent damage caused by ice crystal formation, however, vitrification requires high concentrations (0.1 to 6 M) of non-penetrating cryoprotectants (eg sucrose or trehalose) and rapid cooling, passing rapidly to the glass transition without forming ice crystals. This research evaluated the effect of JR (0.2%) supplemented to freezing (TCG-YH + 5% glycerol) and vitrification (TCG-YH + 100 mM sucrose) media on cryosurvival of bull sperm.
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    Publication
    Effect of L-carnitine in a skimmed milk based-diluent on membranes preservation and kinetic activity of ram sperm under chilled conditions
    (Asociación Interprofesional para el Desarrollo Agrario, 2019) Galarza Lucero, Diego Andrés; Lopez Sebastian, Antonio; Santiago Moreno, Julián; Galarza Lucero, Diego Andrés
    The synergic effect of L-carnitine (LC) and skimmed milk base-extender (UHT) on the kinetic parameters and membranes integrity of cold-stored ram sperm was evaluated. For this purpose, twelve pools of thirty-six semen ejaculates that were collected with artificial vagina from twelve Merino rams were used. After initial evaluation, the semen was divided into 6 aliquots enough to diluted with UHT-extender (control) and 1mM (UHT-LC1), 2.5mM (UHT-LC2.5), 5mM (UHT-LC5), 7,5mM (UHT-LC7.5) and 10mM (UHT-LC10) of LC, respectively, at 200 x 106 cells/ml concentration. Semen samples were cold-stored (5ºC) up to 96 h. Kinetic variables and membranes integrity were assessed by CASA system (SCA) and triple fluorescence association test (IP/PNA-FITC/Mitotracker green). An ANOVA-one way and Bonferroni´s test were used to evaluated effects of LC doses. Overall, the total motility (TM, %), straight line velocity (VSL, μm/s) and total sperm with plasma and acrosome membranes integrity and high mitochondrial function (IPIAHM, %) were greater with all doses of LC (1 to 10 mM) than control group. Also, the UHT-LC5 group provided better values than others LC groups according to motilities. In conclusion, LC improves kinetic vigor and provides an antioxidant protective effect to sperm membranes of cold-stored ram when added to skimmed milk based-diluent
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    Optimization of cryopreservation of arabian stallion sperm using dimethylformamide, glycerol, and different freezing protocols
    (Wiley-Blackwell, 2022) Serpa Carangui, Erika Margoth; Samaniego Campoverde, Jorge Xavier; Galarza Lucero, Diego Andrés; Soria Parra, Manuel Elías; Ochoa Guillén, Juan Diego; Álvarez Palacios, David Alejandro; De la Cruz Tandazo, Francisco Javier; Galarza Lucero, Diego Andrés
    This study evaluated the effect of penetrating cryoprotectant agents (CPA) and the cryosurvival of three freezing protocols on the kinematics and integrity of membranes of frozen-thawed stallion sperm. Twenty-four ejaculates of four adult Arabian horses were collected in six weekly sessions (six ejaculates/horse). Each ejaculate was divided into two aliquots. With the first aliquot, three CPA treatments were conformed: 5% glycerol (GLY), 5% dimethylformamide (DMF), and 3%–3% DMF–GLY combination, and the sperm samples were frozen exposing them to liquid nitrogen (LN2) vapors. The second aliquot was diluted with freezing medium plus 5% DMF and the sperm samples were frozen in three freezing protocols: (P1) Styrofoam cryo-box (30 × 29 × 31 cm of length, width, and height, respectively) with two ramps (at 17 and 7 cm above LN2); (P2) freezing unit® (Minitüb, Germany); and (P3) programmable TK 4000-freezer® (Compacta, Brazil). The DMF-GLY combination and DMF yielded higher (p<.05) post-thaw values than the GLY regarding the motility (SM: 54.2±2.25 and 50.2±1.80 vs. 41.4±2.35%, respectively), curvilinear velocity (VCL: 58.0±1.71 and 54.0±1.58 vs. 42.3±1.60 µm/s), and the proportion of sperm with intact plasma and intact acrosome (IPIA: 58.0±1.11 and 52.6±0.99 vs. 42.5±1.07%). Furthermore, the P1 protocol produced a similar (p>.05) post-thaw SM, VCL, and IPIA than the other protocols. Indeed, the P1 and P3 protocols yielded lower proportion (p<.05) of sperm with damaged plasma and damaged acrosome than the P2 protocol after thawing (3.7±0.18 and 3.1±0.18 vs. 6.1±0.44%, respectively). In conclusion, the addition of DMF or combined with GLY to freezing medium, and the freezing with Styrofoam cryo-box with two ramps increase the cryosurvival of Arabian stallion spermatozoa.
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    Publication
    Cryopreservation of Domestic and Wild Animal Spermatozoa: Update of Knowledge
    (Intechopen, 2023) Samaniego Campoverde, Jorge Xavier; Galarza Lucero, Diego Andrés; Méndez Álvarez, María Silvana; Duma Pauta, José Mauricio
    Current sperm cryopreservation protocols for domestic and wild mammals aim to minimize the cryogenic damage caused by cell dehydration, ice formation, and osmotic stress. The optimization of sperm cryopreservation include the use of different synthetic and nonsynthetic-based extenders supplemented with additives (e.g., egg yolk, coconut water, etc.) and antioxidants (e.g., melatonin, L-carnitine, caffeine, resveratrol, etc.) that protect the plasmalemma, acrosome, and mitochondria against the detrimental effects caused by the cryopreservation process. Furthermore, the use of penetrating (e.g., glycerol, ethylene glycol, dimethylformamide, etc.) and nonpenetrating (e.g., sucrose and trehalose) cryoprotectant agents (CPAs) or their combination should be investigated to protect sperm during the freezing process in slow and ultra-rapid freezing procedures. Finally, new cryopreservation protocols should focus on freezing curves and initial cooling rates that allow optimal dehydration during freezing and adequate hydration during thawing. The suitable interaction of all these factors will allow a sperm subpopulation to survive cryopreservation with integrity and fertilizing capacity, contributing to the improvement of the efficiency of genetic resource management and the development of germplasm banks that support the preservation of genetic diversity in domestic and wild animals.
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    Effect of zeolite on return of ovarian activity, involution and uterine health in postpartum dairy grazing bred cows [Efecto de la zeolita sobre retorno de la actividad ovárica, involución y salud uterina en vacas lecheras postparto criadas en pastoreo]
    (FUNDACION CIPAV, 2017-03-01) Galarza Lucero, Diego Andrés; López Crespo, G. E; Rodriguez Saldana, Diego Fernando; Soria Parra, Manuel Elías
    The objective was to evaluate the effect of natural zeolite in dairy cows for 105 days which included the pre-partum (60 days) and post-partum (45 days) period, on the return of ovarian activity (ROA), uterine involution (UI), uterine health (UH), and body score condition (BSC). Fifty Holstein Friessian grazing cows were used, with ? 3.5 BSC at drying off, between 2 and 5 calving, clinically healthy, with similar sanitary and management conditions. The cows were randomly divided in two groups: control (CG; n1 = 25) fed basal diet; and experimental (EG: n2 = 25) with basal diet + zeolite (2%) of dry matter intake (DMI), equivalent to 180 and 270 g/cow/day, for dry and lactation period, respectively. The ROA was determined by transrectal ultrasonography at 15, 22, 35 and 45 days by the presence of follicles ? 10 mm diameter; UI by rectal palpation at 22 and 45 ppd evaluated by uterus position with respect to the pelvis (UP) and symmetry of the uterine horns (SUH); UH by cytobrush at 35 days; and BSC at 15 days pre-partum, at calving and 45 days post-partum, were evaluated. The zeolite effect was assessed by “U Mann Whitney and Kruscall Wallis” test. The results obtained of the ovarian and uterine variables and BSC were better (P < 0.001) for EG than CG; higher percentage of cows with ROA to 35 days for EG than CG, 52.0% of CG cows and 4% of EG did not return their post-partum ovarian activity; higher percentage of cows that involved her uterus at 45 days regarding UP and SUH; better UH at 35 days expressed by PMN (%); and better BSC at 15 bpd, birth and 45 ppd. Finally, a significant correlation (P < 0.001) between the BSC at 45 days with the percentage of cows that returned their post-partum ovarian activity, UH at 35 days and UI at 45 days. In conclusion, the addition of natural zeolite in the basal diet of grazing dairy cows, before and after calving, was effective in stimulating the return of ovarian activity, improving involution and uterine health, and body score condition, recommending its use in the dairy herds.