Bioquímica y Farmacia-Pregrado

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Browsing Bioquímica y Farmacia-Pregrado by Subject "Aceites Esenciales"

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    Análisis del efecto antibacterial de aceites esenciales de Lepechinia rufocampii y Minthostachys tomentosa sobre cepas de Escherichia coli y Salmonella thyphimurium
    (2013) Calderón Cevallos, Daniela Elizabeth; Guerrero Ricaurte, Ana Isabel; León Tamariz, Fabián
    The use of natural substances for microbial control is one of the areas of study that is growing among these substances are essential oils from different plants. In this study we investigated the antibacterial activity of essential oils and their fractions extracted from Lepechiniarufocampii (Salvia Gateada ) and Minthostachystomentosa ( Pennyroyal large) on bacterial strains of Escherichia coli and Salmonella typhimurium causing multiple foodborne diseases. Essential oils respectively were obtained through a by steam distillation with a Clevenger type trap. Essential oil fractions with positive antibacterial oil were obtained by subjecting to column chromatography and thin layer chromatography. As microbiological analysis , we used a screening through microdilution called plaque, which allows us to a quantification of the minimum inhibitory concentration (MIC) , which is based on the bacterial growth in the presence of various thus concentrations of the essential oil obtained as its fractions. The minimum inhibitory concentrations 50% and 90% (MIC 50 and MIC 90) were the indicators of antibacterial activity, determined by this technique. The results obtained show that both the essential oil Mitostachistomentosa (Poleo large), as certain fractions obtained from the same antibacterial activity against Escherichia coli (220-470 mg / mL) and Salmonella typhimurium (320-776 mg / mL).
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    Determinación del efecto antibacteriano de aceites esenciales obtenidos de la familia Myracaceae y Lamiceae
    (2014) Zaruma Mochas, María Angélica; Illescas Ortega, Juan Pablo; León Tamariz, Fabián; Jerves Andrade, Lourdes; Vega Cuesta, Manuel Guillermo
    Science has real interest in the use of natural substances for microbial control is one of the areas that is booming today, between plants are those that produce essential oils. In this study the antibacterial activity of essential oils extracted from Clinopodiumtenellum( Huarmi Pennyroyal ) , Morella Parvifolia ( Laurel wax ) on strains of S. aureus ATCC 25923 and E. coli ATCC 25922, causing infectious diseases as sought : gastrointestinal and respiratory , respectively. From Clinopodiumtenellum , Morella Parvifolia , essential oils are obtained by distillation by steam with a Clevenger trap . Which were analyzed by gas chromatography using the following five patterns ( alpha-pinene, beta -pinene , linalool , limonene and menthone ) . For microbiological analysis, a screening was performed using the microdilutionplate , with which it is possible to determine the minimum inhibitory concentration (MIC ) in which the activity or negativity is based antimicrobial effect . The essential oils and Morella ParvifoliaClinopodiumtenellum were analyzed at different concentrations. The study demonstrated for the essential oil of Clinopodiumtenellum mild activity at a concentration of 867.8 mg / ml ( MIC 50 ) for E. coli and no activity on S. aureus , and the essential oil of Morella Parvifolia no activity on E. coli and S. aureus.
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    Diseño de mezclas de aceites esenciales como estrategia para el desarrollo de antiamebianos
    (2018) Bustillos Hernández, Esteban Felipe; Cabrera Narváez, Gabriela Isabel; Cazar Ramírez, María Elena
    The use of prepared of medicinal plants to combat diverse problems of health aroused the interest of specify that compound or compound’s group are the responsible to pursue action front diverse microorganism that are able to produce diseases in living beings. Entamoeba histolytica is a parasitic specie that transmit of fecal – oral way and can to generate complications and compromise the health of the people that carry if don’t receive an appropriate treatment. The objective of this assignment was demonstrate that essentials oils (EO) of species of plants that was use in the od as antiparasitic, has action against cysts and amebian pre cysts obtained of fecal samples and maintained viable in a specific cultivation to this specie as Diamond broth. It has been made and qualitative/quantitative experimental research. In which test the action of the Chenopodium ambrosioides (paico), Artemisia absinthium (ajenjo), Ocimum basilicum (albahaca) and Peperomia inaequalifolia (congona) and their combinations to various concentrations through an mixture designs jointly a positive control that only contain with viable amoebas and negative control that it was inhibited the amoebas growth with an pharmacological witness. As result it was demonstrated that the EO of paico, ajenjo and congona are able to inhibit the growth of amoebas in a maximum period of 48 hours and the synergistic effect of their combinations had an anti-amoebic effect after 24 hours.
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    Validación de la técnica de dilución en agar modificada por Katherine A. Hammer, Christine F. Carson, Thomas V. Riley; para la estimación “in vitro” de la actividad antimicrobiana frente a una cepa ATCC de Escherichia coli 25922; y su aplicación a tres aceites esenciales
    (2017-12-21) Sarmiento Ortega, Jaime Marcelo; Romero Chimbo, Silvia Lorena; Jerves Andrade, María de Lourdes
    The antibacterial activity of essential oils (EO) has been the subject of great interest since its beginnings, due to the existence of diverse health problems of bacterial etiology that affect the world population, such as the Escherichia coli (E. coli) pathogen which is one of the most prominent microorganisms in terms of its resistance, developed in response to the need to adapt to new adverse environments and in addition by an indiscriminate use of antibiotics. The objective of this study was to validate the dilution technique in agar modified by Katherine A. Hammer, Christine F. Carson, Thomas v. Riley, with an American Type Culture Collection (ATCC) strain of E. coli 25922, and to determine the possible bioactivity of three EO chosen through bibliographic reviews. This was accomplished by using two EO as controls. The first control EO reacts when introduced to the microorganism, while the second does not. For the development of the study, an experimental design was followed based on a, quantitative/ qualitative approach, in which the following EO were tested: Oregano, Ginger, Rue, Lavender and Lemongrass, using the dilution in agar technique modified by Katherine A. Hammer, Christine F. Carson and Thomas V. Riley. Dilution in agar is a method that has been validated and approved by the Clinical Laboratory Standards Institute (CLSI) and that allows one to prove whether or not an EO reacts when introduced to microorganisms such as Gram (+) or Gram (-). This is possible thanks to small modifications such as the addition of Tween 20, which allows one to overcome surface tension, facilitating the formation of an emulsion between the environment and the EO. Finally, it was possible to validate the technique of dilution in agar modified by Katherine A. Hammer, Christine F. Carson, Thomas V. Riley. The results were: Oregano and Ginger in 0.12% v/v Essential Oil (EO) / Agar Mueller Hinton (MHA), Lavendar 0.48% v/v (EO/MHA), Rue ˃0.48% v/v (EO/MHA) and Lemongrass 0.06% v/v (EO/MHA). The preliminary result can serve as the basis of future studies, due to the enormous potential of EO and the great biodiversity of our region, in order to use them as natural products.