Bioquímica y Farmacia-Pregrado

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Browsing Bioquímica y Farmacia-Pregrado by Author "Aguilar González, Karen Jhovana"

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    Evaluación de la actividad antioxidante de extractos metanólicos y de diclorometano de Jungia paniculata, Jungia coarctata, Jungia fistulosa y Jungia rugosa usando la técnica DPPH y el poder reductor férrico
    (2015-10-21) Aguilar González, Karen Jhovana; Tenorio Arévalo, María Caridad; Wilches Arizábala, Isabel María
    In this research the antioxidant capacity from Jungia rugosa, Jungia coarctata, Jungia paniculata and Jungia fistulosa leaves was evaluated using DPPH (2, 2 -diphenyl-1-pycrylhidrazyl) and reducing power (reduction of ferricyanide) techniques. DPPH assay evaluates bleaching radical (DPPH) by action of an antioxidant and technique ferric reducing power the capacity of a reducing agent to react with potassium ferricyanide and produce potassium ferrocyanide. Ascorbic acid was used as standard wich IC50 was 3.07 µg/ml in the art of DPPH. In the DPPH test, the evaluation of the antioxidant activity from the extracts and the DCMs, was carried out using the IC50 (concentration to which 50% of free radicals are inhibited), obtaining values between 71,09-352,86 µg/ml µg/ml for methanol and between 7,55-182,29 µg/ml for DCM. For the reducing power test, the evaluation of the antioxidant activity from the extracts and the DCMs, was carried out by the AAEAC (milligrams of ascorbic acid equivalent to 1 gram of dry drug), obtaining values between 6, 58-271,09 mg/g for methanol and between 2,05-72,29 mg/g for DCM. Through an ANOVA test and a Tukey test it was proved that there are differences in the antioxidant capacity between species in both antioxidant techniques. Using a thin layer chromatography, the identification of metabolites of different species of Jungia was performed, finding the phenolic compounds as the main substances in granting the antioxidant properties of these plants. The findings suggest that the extracts analyzed from species Jungia have antioxidant activity, attributed to the presence of phenolic compounds and terpenes.