Facultad de Ciencias Químicas-Tesis Maestrías

Permanent URI for this collectionhttps://dspace-test.ucuenca.edu.ec/handle/123456789/296

Browse

Browsing Facultad de Ciencias Químicas-Tesis Maestrías by Author "Abad Terán, Mónica Eulalia"

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Evaluación del riesgo toxicológico de la acrilamida del café de las marcas más consumidas en la zona urbana de la ciudad de Cuenca
    (2019-05-29) Uday Santacruz, Fabiola Carolina; Abad Terán, Mónica Eulalia
    One of the steps in the production of granulated soluble coffee is the toasting of coffee beans at high temperature. This procedure leads to the formation of the toxic acrylamide, through the so-called Maillard reaction. Based on this, the search aimed to evaluate the toxicological risk of acrylamide in coffee of the most consumed brands in the urban area of Cuenca. Based on 384 surveys conducted to adult coffee consumers, the six most preferred brands were found, three for granulated coffee –traditionally known as filtered coffee - and three for soluble coffee. In addition, it was possible to determine the daily amount of coffee consumed by the respondents. Once the most recognized coffee brands in each category were identified, twelve samples were collected by the EPA8032a method modified by chromatography gasses with GC/ECD. In all samples the concentration of acrylamide were below the detectable limit (<0.02 mg/kg), which did not allow adequately establishing the toxicological risk for the consuming population. Likewise, a Margin of Exposure (MOE) greater than 10000 was obtained, which means that the coffee of the studied brands did not represent any toxicological risk of consumption.
  • Thumbnail Image
    Item
    Fitotoxicidad del material particulado sedimentable (MPS) generado en la zona urbana del cantón Cuenca
    (2014) Abad Terán, Mónica Eulalia; García Alvear, Nancy Beatriz
    In this work the phytotoxicity of Sedimentable Particulate Material (acronym in spanish: MPS) generated in the urban area of canton Cuenca was determined by bioassay, determining if the MPS concentration is so abundant and if its composition contains toxic substances enough to affect the normal development of plants. Bioassays are conducted with the assumption that the organisms tested are keys to higher organisms in the natural environment (Escalante, E. 2000). The MPS was collected at three sites in the urban area of canton Cuenca, by the method described in the Unified Text of Secondary Environmental Legislation of the Ministry of Environment (acronym in spanish: TULASMA). It is known that particulate matter are present in a variety of toxic substances including heavy metals and anions (CEA. 2009), so that the determination of the concentration of MPS was done using gravimetric analysis, by atomic absorption spectroscopy for heavy metals, and by the adapted method with reference EPA 300.1 for anions. Subsequently phytotoxicity was evaluated in four species by bioassay of germination and root- and hypocotyl-elongation, with a method adapted by Sobrero M. (2005), Guide 850.4200 USEPA-OPPTS, in seeds of Lactuca sativa, Brassica oleraceaitalica, Allium cepa, Raphanussativus. In the bioassay seeds were exposed to different dilutions of the aqueous suspension of MPS, the results were dependent on the concentration and the tested specie, showing different levels of phytotoxicity.