Determinación de los parámetros cinéticos como: la velocidad máxima de reacción y la constante de crecimiento en el proceso fermentativo del mucílago de cacao CCN-51 caso Ecuador, Zona 6

Abstract

In the present work of qualification, the primary objective is the obtaining and analysis of kinetic parameters of the equation of Monod: maximum specific growth rate (μ_max) and Monod constant (K_S) from the mathematical models of Lineweaver - Burk, Eadie Hofstee and Hanes Woolf of cocoa CCN - 51, mucilage fermentation processes applied to the fermentation process of the mucilage of cocoa CCN - 51, keeping constant the amount of 3 g/l yeast Sacchromyces Cerevisiae and varying the initial concentration of ° Brix, working with 8.5; 17; 21 and 24 ° Brix. Experimentation developed in the bioreactor Bath Biotron GX Single Vessel with parameters of pH = 4 and temperature = 35° C. The generated biomass quantification was carried out through the gravimetric method weight dry and lyophilization, and applied the phenol - sulfuric acid (Dubois) method to quantify the amount of substrate consumed. Manipulation of greater preponderance variables are specific growth rate (μ) obtained from initial and final biomass and substrate consumption; from the mentioned variables graphics obtained for different models of linear regression are handled according to their applicability. The data obtained of K_S y μ_max in the study are: 47,6889 (g/l) y 0,1202 (h-1) respectively, those were obtained through linear regression methods proposed, checking the major method to adjust linear is the Hanes-Woolf resulting coefficient of linear correlation 0,9937. The results obtained were compared with those obtained in previous investigations by programming in the software Matlab Version 8.3.