Caracterización molecular de las cepas de Parvovirus canino tipo 2 en perros de la ciudad de Cuenca

Abstract

The canine parvovirus is a contagious disease caused by canine parvovirus type 2 (CPV-2). It especially affects puppies under six months and adult dogs without immune protection. The signology includes lethargy, anorexia, fever, vomiting and diarrhea; if the animal doesn’t get support treatment, the illness can be lethal. The original strain CPV-2 has been completely replaced by three variants (CPV-2a, CPV-2b, CPV-2c), and this evolution has occurred in a relatively short time. However, there is controversy regarding how virulent these strains are, as well as the level of protection of the current vaccines. The aim of this study was to identify the different strains of CPV-2 in dogs with parvovirosis compatible signs in the city of Cuenca. For this, 80 feces samples were collected, from which those that did not generated a specific amplicon of dog (Process control) were discarded. Subsequently, the disease diagnostic PCR was performed and 25 positive samples were selected and sent to sequencing. From these samples, it was possible to obtain the genetic sequence of 21. The 47,61% belonged to strain CPV-2a, followed by strain CPV-2c with 38.1%, and finally the strain CPV-2b with 14,29%. According to what has been published, these strains suffer prevalence changes and can generate subvariants of itselfs over the time and in different geographic locations.