Efecto del resveratrol sobre la criosupervivencia de espermatozoides equinos congelados y vitrificados

Abstract

The research assessed the impact of Resveratrol (RES) on the cryosurvival of equine sperm using conventional freezing (C) and kinematic vitrification (V). Sixteen semen samples from 4 Spanish horses were collected and divided into four groups: C-RES, C-Co, V-RES, and V-Co, based on the cryopreservation method (C or V) and RES presence (10 or 0 μM). During freezing, 0.5 mL straws with 5% dimethylformamide were used, while vitrification employed 100 mM trehalose and submersion in 30 μL drops in LN2. Results showed that

RES treatments, especially C-RES, enhanced total and progressive motility compared to V- RES and V-Co. Additionally, curvilinear velocity (VCL) was higher in C-RES and C-Co than

in V-RES. Conversely, linearity (LIN) and lateral oscillation (WOB) values were higher in V- RES and V-Co treatments. Increased levels of plasma and acrosomal integrity were

observed in C-RES, with lower DNA fragmentation in these groups. An antioxidant effect of RES was also detected, reducing oxidative stress in C-RES compared to C-Co. In conclusion, RES improved equine sperm cryosurvival, as evidenced by enhanced motility and cellular integrity, along with reduced DNA fragmentation and oxidative stress. These findings suggest the potential use of RES as an additive in equine semen cryopreservation protocols to enhance the efficacy of assisted reproduction in horses