“Caracterización genética de gallos de pelea (Gallus gallus) del cantón Cuenca mediante mtDNA D-Loop.”

Abstract

The objective of this research was to genetically characterize by means of mtDNA D-loop, the fighting roosters in the different hatcheries of the Cuenca canton, province of Azuay, Ecuador. A pilot sample was carried out in 10 farms in the canton to determine the existence of ecotypes of fighting cocks and quantify their respective troops. For the ecotype criteria, the breeder's information, external bird morphology, the work of comparing photos with bibliographic references and the criteria of an expert were taken into account. Subsequently, 15 birds were selected from the three most abundant and most distributed ecotypes in the hatcheries visited; in which they stand out: Ecuadorians, Chileans and Puerto Ricans from which feathers of each animal were taken. In addition, DNA from Cuban roosters was included, (out). 𝐹st, Nst and AMOVA were determined. The ecotypes of Ecuadorian, Chilean, Puerto Rican and Cuban roosters showed little variability among them, 5%. The greatest variability was found within each ecotype. In the 19 haplotypes in only one, the 4 ecotypes are represented, most of them are represented by a single individual. In the genetic parameters such as: segregation sites, number of haplotypes, diversity of haplotypes and average differences in the number of nucleotides, the Cuban ecotype presented values higher than the remaining ecotypes.