Caracterización de los genotipos frecuentes del virus del Papiloma Humano en mujeres atendidas en los hospitales Monte Sinai y del Seguro Social Cuenca- Ecuador. 2008- 2014

Abstract

OBJECTIVE: To determine the prevalence of human papilloma virus genotypes in cervical-uterine samples and their relation to risk factors in women with active sex life in Cuenca. METHODOLOGY: It is a cross-sectional observational study with a sample of 500 women from Cuenca who were attended in the Monte Sinai Hospital and the Ecuadorian Institute of Social Security (IESS). The exams were carried out in the BIONCOGEN Molecular Biology Laboratory which is located in Cuenca. The Real Time PCR technique plus the Pap examination were used. RESULTS: The prevalence of HPV was 78.4 % and the most frequent genotype was the HPV 16 with 26.2 %. On the other hand, the CIN I with 27% is the most frequent gynecological pathology. CONCLUSION: The genotypes 16/18 represent the types of HPV that are directly related to the development of cervical cancer because when it is correlated with cytology (Pap) it is demonstrated that the cell undergoes morphological changes (koilocytosis). Keywords: Genotype, Human papillomavirus 16, Human papillomavirus 18, Clinical Laboratory Techniques, PCR; Risk factors; Monte Sinai Hospital, Hospital of the Ecuadorian Institute of Social Security, Cuenca-Ecuador.