Determinar la carga bacteriana persistente post-desinfección empleando el medio de cultivo agar sangre en superficies de cinco áreas diferentes de clínicas veterinarias de la ciudad de Cuenca

Abstract

The main objective of this study was to determine the persistent bacterial load (UFC) on different post - disinfection surfaces at small species veterinary centers in order to evaluate which disinfection protocol is better at reducing the UFC. The research was carried out in 20 veterinary clinics in the city of Cuenca, province of Azuay, which had specific areas for the study: hospitalization, operating room, consultation, laboratory and secretarial area. In order to obtain information about the disinfection protocols used by each clinic, a survey was carried out followed by the sampling of each area, finally obtaining 100 samples. Bacterial culture was carried out on blood agar in a private laboratory, which lasted 72 hours for the identification of CFU. Through the descriptive statistical analysis, it was concluded that there was not bacterial growth in 84% of the samples taken. Based on the results, it was determined the presence of bacterial load in the hospitalization and consultation areas. Finally, the veterinary clinics that used 4 or more disinfection products in their protocols did mi showed the absence of bacterial proliferation.