Determinación de la actividad antimicrobiana de jungia rugosa less en extractos de n-hexano y diclorometano

Abstract

Jungiarugosa Less is a native plant from Ecuador, its leaves had been used since ancient age for people from Andes due to the anti-inflammatory and cicatrizing effects that this medicinal plant has showed. This study was conducted to determine the antibacterial activity from extracts of leaves, flowers and stems of Jungiarugosa Less in dichloromethane and n-hexane against bacteria such us Staphylococcus aureus(ATCC 25923), Escherichia coli (ATCC 25922 )and Salmonella typhimurium(ATCC 14028). The extracts were obtained by percolation and concentrate under vacuum. TLC (Thin Layer chromatographic) method was performed determining the presence of flavonols and flavones only in the leaves extracted with dichloromethane, and the presence of terpenoid compounds and coumarins in leaves, flowers and stems extracted with dichloromehane and n-hexane. To determinate the antibacterial activity three different technique were applied: microdilution plate, dilution plate and agar diffusion, however it was not possible to obtain results about the antibacterial activity by the lack of solubility of the extracts, due to its high resinous character which resulted in a lack of reproducibility of the results.