Efecto del extracto del Aloe vera (Aloe barbadensis miller) en la congelabilidad del semen porcino

Abstract

The objective of this research was to evaluate the effect of the addition of Aloe vera extract in a cryopreservation medium on the freezing of porcine semen. We used 16 ejaculates of 4 sexually mature boars, from 1 to 3 years, adapted and maintained in a farm in the Guachapala canton, the seminal analyzes and the freezing was carried out in the laboratory of Biotechnologies of the Animal Reproduction of the University of Cuenca; The ejaculates were divided into two aliquots for the treatments: T1.- Freezing of porcine semen in diluent based on sodium citrate and egg yolk; And, T2.- Freezing of porcine semen in diluent based on sodium citrate, egg yolk plus 10% v / v of Aloe Vera in the preparation water. Post-freeze parameters were: progressive individual motility, vitality and membrane integrity (HOS Test). A completely random design was used. Statistical analysis was performed with Student's t test and Mann-Whitney U test. There were no significant differences between the treatments (P> 0.05) on the variables: motility, vitality and abnormalities; However, HOS Test showed higher percentages (P <0.05) in T2 compared to T1 (respectively: 15.54 ± 0.50% and 13.89 ± 0.65%). It is concluded that the addition of the Aloe vera extract to 10% v / v of the media preparation water produced no difference in the parameters of motility, viability; However, decreased the deleterious effects of cryopreservation on the integrity of the plasma membrane of porcine spermatozoa