Evaluación de la actividad inhibitoria de las enzimas A y B glucosidasa en frutos de la sierra sur ecuatoriana

Abstract

The present research is focused on the assessment of the inhibition capacity of aqueous extracts from Physalis peruviana, Malus communis and Solanum betaceum, on the enzymes α and β glucosidase The fruits under study were available in three Cuenca city markets. An "in vitro" assay of inhibition enzymatic activity was applied by means of the microdilution technique in 96-wells plates. In this assay, Physalis peruviana (uvilla) displayed the highest inhibitory activity towards the enzymes α and β glucosidase (65.70% and 68.89%, respectively). Solanum betaceum (tree tomato) presented medium inhibitory level (47.4% for α-glucosidase and 57.83% for β-glucosidase). Malus communis (apple) displayed a modest inhibitory activity towards α and β glucosidase (35.17% and 35.52%, respectively). Significant differences between the enzymatic activity of the fruits were evaluated, related to the place of commercialization, by means of a one-way Analysis of Variance. The fruits under study, gathered a three markets in Cuenca city, present similar enzymatic activity (p ≥ 0.05). The results obtained in this study highlight the potential of the fruits included as a source of bioactive inhibitors of α and β glucosidase. These findings might imply a further study as nutraceutical supplements for a healthy diet.