BoviPure® density-gradient centrifugation procedure enhances the quality of fresh and cryopreserved dog epididymal spermatozoa
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Date
2022
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Abstract
BoviPure® is a salt solution containing colloidal silica particles coated with silane used to select sperm (e.g., ruminants) by density-gradient centrifugation (DGC). This research assessed the suitability of the BoviPure-DGC and swim-up methods for selecting dog epididymal sperm in fresh, chilled and frozen-thawed samples on post-treatment sperm quality. Sperm samples (n = 60 epididymides) were recovered by retrograde flushing from thirty orchiectomized adult dogs. Thereafter, 20 sperm pools, containing sperm aliquots of three randomly selected animals, were used for chilling (at 5 ºC for 24 h) and freezing (in liquid nitrogen vapors). Sperm selection by BoviPure-DCG and swim-up was performed in both individual and pooled samples, including non- selected samples as controls. Overall, after BoviPure-DGC selection a higher sperm retrieval rate was obtained than the swim-up selection in both individual (P < 0.05) and pooled (P < 0.01) samples. BoviPure-DGC improved (P < 0.05) the total (TM) and progressive (PSM) sperm mo- tilities, curvilinear (VCL) and straight-line (VSL) velocities, linearity (LIN), wobble (WOB), beat- cross frequency (BCF), and integrity of plasmatic (IPM) and acrosomal (IAM) membranes of in- dividual samples in comparison with non-selected samples. In pooled samples, however, the BoviPure-DGC improved (P < 0.05) the PSM, VCL, WOB, and IPM of chilled and frozen-thawed samples. The swim-up method improved (P < 0.05) only some kinematic variables of the indi- vidual (VCL, WOB and BCF) and cryopreserved pooled samples (VCL and ALH) in comparison with non-selected samples. In conclusion, BoviPure-DGC was more effective for recovering and selecting both fresh and cryopreserved dog epididymal sperm than the swim-up procedure improving the kinematic variables, and membranes intactness
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Keywords
Sperm selection, Epididymal sperm, BoviPure, Dogs, Swim-up
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http://dspace.ucuenca.edu.ec/handle/123456789/39094
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https://www.scopus.com/record/display.uri?eid=2-s2.0-85131145132&origin=resultslist&sort=cp-f&src=s&st1=BoviPure%c2%ae+density-gradient+centrifugation+procedure+enhances+the+quality+of+fresh+and+cryopreserved+dog+epididymal+spermatozoa&sid=8af471bdd14ce7d21da4e32eae2423f6&sot=b&sdt=b&sl=141&s=TITLE-ABS-KEY%28BoviPure%c2%ae+density-gradient+centrifugation+procedure+enhances+the+quality+of+fresh+and+cryopreserved+dog+epididymal+spermatozoa%29&relpos=0&citeCnt=0&searchTerm=&featureToggles=FEATURE_NEW_DOC_DETAILS_EXPORT:1