Competencia del ovocito bovino obtenido por ovum pick-up valorado mediante el azul brillante de cresilo

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dc.contributor.authorAyala Guanga, Luis Eduardo
dc.contributor.authorSamaniego Campoverde, Jorge Xavier
dc.contributor.authorNieto Escandón, Pedro Emilio
dc.contributor.authorRodas Carpio, Ermes Ramiro
dc.contributor.authorDután Sanango, Jorge Bolívar
dc.contributor.authorCalle Ortiz, Guido Rigoberto
dc.contributor.authorMurillo Apolo, Yury Agapito
dc.contributor.authorVázquez Mosquera, Juan Mesías
dc.contributor.authorArgudo Garzón, Daniel Ernesto
dc.contributor.authorPerea Ganchou, Fernando Pedro
dc.date.accessioned2018-06-16T14:41:00Z
dc.date.available2018-06-16T14:41:00Z
dc.date.issued2018
dc.descriptionThe objective of the present study was to evaluate the bright blue cresyl (BCB) test as an indirect method to select competent oocytes for the in vitro production of embryos (IVP). The cumulus-oocyte complexes (COC) were obtained from two Creole heifers subjected to two treatments: T1 = COC recovered by OPU (ovum pick-up) previous stimulation with FSH-LH; T2 = COC recovered from non-stimulated animals (control).The two heifers were alternated in the two treatments and five repetitions were done. Recovered COCs were classified into types A, B, C and D. Then the BCB test was applied to each of the COC types to determine if they were BCB+ or BCB-. T1 allowed to recover 5.2 more COC than T2 (p<0.05). When applying the BCB test, it was determined that all type A oocytes of T1 and T2 were BCB+; that is, they finished their growth and were ready to start the process of in vitro maturation; however, about 50% of the type B, C and D COCs of T1 and T2 were BCB+. It is concluded that the selection of COC based on morphological characteristics is a reliable method only for type A and has a 50% error for COC type B, C and D and, therefore, the application of the BCB test allows to improve this selection non-invasively
dc.description.abstractThe objective of the present study was to evaluate the bright blue cresyl (BCB) test as an indirect method to select competent oocytes for the in vitro production of embryos (IVP). The cumulus-oocyte complexes (COC) were obtained from two Creole heifers subjected to two treatments: T1 = COC recovered by OPU (ovum pick-up) previous stimulation with FSH-LH; T2 = COC recovered from non-stimulated animals (control).The two heifers were alternated in the two treatments and five repetitions were done. Recovered COCs were classified into types A, B, C and D. Then the BCB test was applied to each of the COC types to determine if they were BCB+ or BCB-. T1 allowed to recover 5.2 more COC than T2 (p<0.05). When applying the BCB test, it was determined that all type A oocytes of T1 and T2 were BCB+; that is, they finished their growth and were ready to start the process of in vitro maturation; however, about 50% of the type B, C and D COCs of T1 and T2 were BCB+. It is concluded that the selection of COC based on morphological characteristics is a reliable method only for type A and has a 50% error for COC type B, C and D and, therefore, the application of the BCB test allows to improve this selection non-invasively
dc.identifier.doi10.15381/rivep.v29i2.13816
dc.identifier.issn1682-3419
dc.identifier.urihttp://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13816
dc.language.isoes_ES
dc.sourceRevista de Investigaciones Veterinarias del Perú
dc.subjectBovine Coc
dc.subjectOocyte Morphology
dc.subjectBcb
dc.subjectG6pdh
dc.titleCompetencia del ovocito bovino obtenido por ovum pick-up valorado mediante el azul brillante de cresilo
dc.title.alternativeCompetence of the bovine oocyte obtained by ovum pick-up as evaluated by the bright cresyl blue test
dc.typeARTÍCULO
dc.ucuenca.afiliacionAyala, L., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
dc.ucuenca.afiliacionSamaniego, J., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
dc.ucuenca.afiliacionNieto, P., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
dc.ucuenca.afiliacionRodas, E., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
dc.ucuenca.afiliacionDutan, J., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
dc.ucuenca.afiliacionCalle, G., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
dc.ucuenca.afiliacionMurillo, Y., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
dc.ucuenca.afiliacionVazquez, J., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
dc.ucuenca.afiliacionArgudo, D., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
dc.ucuenca.afiliacionPerea, F., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
dc.ucuenca.areaconocimientofrascatiamplio4. Ciencias Agrícolas
dc.ucuenca.areaconocimientofrascatidetallado4.4.2 La BioTecnología y los Alimentos
dc.ucuenca.areaconocimientofrascatiespecifico4.4 Biotecnología Agricola
dc.ucuenca.areaconocimientounescoamplio08 - Agricultura, Silvicultura, Pesca y Veterinaria
dc.ucuenca.areaconocimientounescodetallado0841 - Veterinaria
dc.ucuenca.areaconocimientounescoespecifico084 - Veterinaria
dc.ucuenca.correspondenciaAyala Guanga, Luis Eduardo, luis.ayala@uceunca.edu.ec
dc.ucuenca.cuartilQ3
dc.ucuenca.factorimpactoH4
dc.ucuenca.idautor0102635463
dc.ucuenca.idautor0105163489
dc.ucuenca.idautor0102840576
dc.ucuenca.idautor0102979424
dc.ucuenca.idautor0301093647
dc.ucuenca.idautor0300977238
dc.ucuenca.idautor0702650136
dc.ucuenca.idautor0103055554
dc.ucuenca.idautor0104461165
dc.ucuenca.idautor0151638103
dc.ucuenca.indicebibliograficoSCOPUS
dc.ucuenca.numerocitaciones0
dc.ucuenca.urifuentehttp://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria
dc.ucuenca.versionVersión publicada
dc.ucuenca.volumenvolumen 29, número 2

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