Detección del gen blaSHV en enterobacterales productores de betalactamasas de espectro extendido en cepas procedentes de la Clínica Santa Ana y Hospital del Río, Cuenca 2024

Abstract

Background: Infection caused by extended-spectrum beta-lactamase (ESBL)-producing bacteria represent a public health challenge due to expensive alternative therapies. Therefore, detection of the blaSHV gene in these bacteria is significant to understand the molecular epidemiology, since it may provide a solid scientific basis for designing strategies to mitigate its dissemination an impact on the population. Objective: To detect the presence of the blaSHV gene in extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales strains from Santa Ana Clinic and Del Rio Hospital and their subsequent phenotypic confirmation of ESBL production by double-disc test for detection of the blaSHV gene, through conventional polymerase chain reaction. Results: The blaSHV gene was detected in 15 (17,60%) strains, mainly in Klebsiella pneumoniae 10 (11,80%), followed by Escherichia coli 3 (3,50%), Proteus mirabilis 1 (1,20%), and Morganella morganii 1 (1,20%). Most isolates came from urine samples (17,60%). Conclusions: The blaSHV gene was responsible for 17.60% of ESBL production in the strains analyzed. 100% of them showed positive results in the double-disk test, and Klebsiella pneumoniae was the main carrier of the gene (11,80%).