Establecimiento de un método de extracción y detección de cianotoxinas a partir de cultivos de Oscillatoria sp. obtenidos en condiciones de estrés

Abstract

Cyanobacteria are microscopic organisms that can grow up in different environments, both aqueous and terrestrial. Its presence can constitute a risk for other living organisms when its reproduction is uncontrolled, forming the so-called blooms. This activity is based on its ability to produce subst ances, especially nitrogenous (peptides or alkaloids) that are called cyanotoxins. The present work seeks to establish general parameters for the extraction and determination of cyanotoxins from Oscillatoria sp cultures obtained under conditions of light stress, excess nutrients, and pH variation. The cultures obtained under these conditions were compared with cultures produced under standardized conditions. Thalassiosira sp. as a species of microalgae or negative control. The extracts were obtained by treatment by micronization and ultrasound. They were then purified by solid phase extraction, and subsequently, analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The analysis of results does not allow observing the presence of cyanotoxins in the purified fractions. However, the analytical limitations present in this work do not provide conclusive results.