Detección y genotipificación molecular del virus de papiloma bovino en lesiones de animales afectados por papilomatosis cutánea

Abstract

Bovine papillomatosis is an infectious-transmissible disease caused by one or more of the 15 types of bovine papilloma virus, the lesions are characterized by scattered neoplastic growths or in groups located in keratinized and nonkeratinized body epithelia. As well as in the mucous surfaces of the digestive and genitourinary tract, the latter related to the chronic consumption of ferns of the genus Pteridium. In the present work, the genetic material of bovine papilloma virus and its genotyping was identified using molecular biology techniques (PCR and RFLP) for which 40 postmortem samples were collected from lesions suggestive of papillomatosis bovine, of 30 cattle slaughtered in the camels of Cuenca, Paute and Azogues. In addition, 13 antemortem samples were collected from 11 animals from the province of El Oro. Positive samples correspond to lesions present in the neck, udder, canopy handset The most frequently encountered types were 3 and 6, followed by types 7 and 9. In addition, 4 types of restriction patterns were visualized, one of which was repeated in 7 of the 18 positive samples, the same as no correspond to the predicted patterns for each of the 15 types of BPV. With the detection and genotyping of bovine papilloma virus types, a multivalent vaccine can be carried out as a prophylactic and therapeutic tool specific to the types of BPV circulating in Ecuador.