Valoración de ovocitos obtenidos mediante Ovum pick-up en vaquillas sometidas a protocolos alternativos de sincronización de la onda folicular y estimuladas con FSH-LH

Abstract

The objective of the present study was to evaluate the quantity and quality of oocytes retrieved by Ovum pick-up (OPU) of native Holstein heifers, synchronized by three alternative protocols for restarting the follicular wave, followed by ovarian stimulation with (FSH-LH). The experimental units (n=9) were divided into three groups and interspersed in three treatments (T1= follicular ablation, T2= GnRH, T3= estradiol benzoate). A standard synchronization protocol was used prior to each repetition to homogenize its estrous cycle (day 0: progesterone implant 0.5g + 2mg BE, day 7: 0.5mg prostaglandin F2α + estrus detection patch + implant removal, day 8: 1mg BE). On the day of restarting the follicular wave in each treatment, 500 IU of FSH-LH were applied intramuscularly. 48 hours later, the OPU was performed. (Three sessions were conducted during the study at intervals between sessions of 21 days). The data were processed in the statistical program SPSS version 24, by means of the ANOVA test for the quantitative variables and Chi square to relate percentages. No statistical differences were found in the mean number of follicles visualized (11.7, 9.8, 9.0, respectively, P> 0.05) or in the percentage of recovered oocytes (52.4%, 55.7%, 61.7%, respectively, P> 0.05) or in the averages of the cumulus oocyte complexes (COC) (47.3%, 57.1%, 52.0%, respectively, P> 0.05). In conclusion, ovarian stimulation with FSH-LH at the time of the restart of the follicular wave, reached a similar average in the number of follicles visualized, as well as in the percentages of recovery of COC, for the production of embryos in vitro, regardless of the method used to restart the follicular wave