Adición de suero de cobaya en celo a un medio de maduración in vitro

Abstract

The work was carried out in the Biotechnology laboratory of the Faculty of Agricultural Sciences of the University of Cuenca. The objective of this study was to evaluate the effect of different percentages of guinea pig serum in heat (SCC) as a supplement in an in vitro maturation medium (IVM) for guinea pig oocytes from slaughterhouse ovaries. For this, in the first instance, 10 crossbred guinea pigs were synchronized, the day they showed heat, blood serum was obtained. In the second instance, slaughterhouse ovaries were collected and COC's were obtained from these using the Slicing technique, these were classified into type A, B and C based on their morphology. Three treatments were evaluated: T0=control (base maturation medium), T1=base medium + 5% SCC supplementation, T2= base medium + 10% SCC supplementation, T3= base medium + 20% supplementation. % of SCC it was determined that the addition of SCC caused a greater degree of expansion of cumulus cells 5% (84.65 ± 3.98), 10% (84.49 ± 3.63) and 20% (87.43 ± 3.98) with respect to control (69.7 ± 3.63; p<0.005). The percentage of oocytes that reached metaphase II in T1 (25.91 ± 2.79), T2 (26.9 ± 2.79) and T3 (32.48 ± 3.06), were higher compared to the T0 (8.65 ± 2.79; p<0.05). In conclusion, the oocytes that were matured in medium supplemented with SCC presented a better percentage of morphological maturation than cumulus cells and oocytes that reached metaphase II.