Person:
Bigoni Ordóñez, Gabriele Davide

Birth Date

1983-08-30

ORCID

0000-0003-2091-6107

Scopus Author ID

57204013087

Afiliación

Universidad de Cuenca, Cuenca, Ecuador
Universidad de Cuenca, Facultad de Ciencias Médicas, Cuenca, Ecuador

País

Ecuador

Organizational Units

Organizational Unit

Facultad de Ciencias Médicas

La Facultad de Ciencias Médicas de la Universidad de Cuenca fue creada en el año 1867, convirtiéndose en una de las facultades más antiguas. La Facultad de Ciencias Médicas es un lugar donde la pasión por la salud y el compromiso con el bienestar se fusionan para formar profesionales altamente capacitados en diversas disciplinas del área de la salud. Nos enorgullece ofrecer un amplio programa académico de calidad que abarca varias áreas importantes para el bienestar de nuestra comunidad. Ofrecemos un entorno de aprendizaje enriquecedor y vanguardista, con docentes altamente capacitados y recursos actualizados. Valoramos la vinculación con lo colectivo y la investigación científica, con compromiso social al igual que ética. Nuestros graduados son reconocidos por su excelencia y están preparados para afrontar los desafíos de un mundo en constante evolución.

Job Title

Profesor (T)

Last Name

Bigoni Ordóñez

First Name

Gabriele Davide

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Search Results

Now showing 1 - 10 of 10

The Hallmarks of Cervical Cancer: Molecular Mechanisms Induced by Human Papillomavirus
(2024) Ortiz Segarra, José Ignacio; Vega Crespo, Bernardo José; Bigoni Ordóñez, Gabriele Davide
Human papillomaviruses (HPVs) and, specifically, high-risk HPVs (HR-HPVs) are identified as necessary factors in the development of cancer of the lower genital tract, with CaCU standing out as the most prevalent tumor. This review summarizes ten mechanisms activated by HR-HPVs during cervical carcinogenesis, which are broadly associated with at least seven of the fourteen distinctive physiological capacities of cancer in the newly established model by Hanahan in 2022. These mechanisms involve infection by human papillomavirus, cellular tropism, genetic predisposition to uterine cervical cancer (CaCU), viral load, viral physical state, regulation of epigenetic mechanisms, loss of function of the E2 protein, deregulated expression of E6/E7 oncogenes, regulation of host cell protein function, and acquisition of the mesenchymal phenotype.
Características biológicas y moleculares de la CD66c e importancia en el pronóstico de la leucemia linfoblástica aguda
(2024) Bigoni Ordóñez, Gabriele Davide
This study aims to gather information on the characteristics and functions of the CD66c molecule through a literature review, as well as its prognostic role when expressed in acute lymphoblastic leukemia (ALL). ALL is a disease originating from the malignant proliferation of hematopoietic cells. It is the most common cancer in the pediatric population, with a survival rate exceeding 70% globally in developed countries. In adult patients, the disease most frequently occurs between the ages of 20 and 40, with an unfavorable prognosis in this population. Several studies have demonstrated the presence of the glycoprotein CD66c in ALL, describing it as a potential marker related to genetic alterations such as BCR-ABL1, hyperdiploidy, and TEL-AML1 fusion gene negativity. CD66c is a member of the carcinoembryonic antigen (CEA) family, expressed in the granulocytic lineage, and is involved in functions such as cell adhesion, regulation of gene expression, migration, and signal transduction.
Integrin α6 (CD49f), the microenvironment and cancer stem cells
(2019) Czarnowski, Daniel; Gerard J., Madlambayan; Tyler, Parsons; Bigoni Ordóñez, Gabriele Davide; Luis G., Villa Diaz
Cancer is a highly prevalent and potentially terminal disease that affects millions of indi- viduals worldwide. Here, we review the literature exploring the intricacies of stem cells bearing tumori- genic characteristics and collect evidence demonstrating the importance of integrin 6 (ITGA6, also known as CD49f) in cancer stem cell (CSC) activity. ITGA6 is commonly used to identify CSC popula- tions in various tissues and plays an important role sustaining the self-renewal of CSCs by interconnect- ing them with the tumorigenic microenvironment.
Cancer stem cell impact on clinical oncology
(2018) Toledo Guzmán, María Esperanza; Ortiz Sánchez, Elizabeth; Ibañez Fernández, Miguel; Bigoni Ordóñez, Gabriele Davide
Cancer is a widespread worldwide chronic disease. In most cases, the high mortality rate from cancer corre- lates with a lack of clear symptoms, which results in late diagnosis for patients, and consequently, advanced tumor disease with poor probabilities for cure, since many patients will show chemo- and radio-resistance. Several mechanisms have been studied to explain chemo- and radio-resistance to anti-tumor therapies, including cell signaling pathways, anti-apoptotic mecha- nisms, stemness, metabolism, and cellular phenotypes. Interestingly, the presence of cancer stem cells (CSCs), which are a subset of cells within the tumors, has been related to therapy resistance. In this review, we focus on evaluating the presence of CSCs in different tumors such as breast cancer, gastric cancer, lung cancer, and hematological neoplasias, highlighting studies where CSCs were identified in patient samples. It is evident that there has been a great drive to identify the cell surface phenotypes of CSCs so that they can be used as a tool for anti-tumor therapy treatment design. We also review the potential effect of nanoparticles, drugs, natural compounds, aldehyde dehydrogenase inhibitors, cell signaling inhibitors, and antibodies to treat CSCs from specific tumors. Taken together, we present an overview of the role of CSCs in tumorigenesis and how research is advancing to target these highly tumorigenic cells to improve oncology patient outcomes.
CD20, generalidades básicas-moleculares y su posible relación como marcador de mal pronóstico en leucemia
(2023) Bastidas Sánchez, Andrea Natali; Bigoni Ordóñez, Gabriele Davide
CD20 is a transmembrane protein expressed on the surface of the B lymphocyte and plays a significant role in its development and differentiation. It is expressed in most B-cell neoplasms, such as Acute Lymphoblastic Leukemia (ALL). Information was collected on the biological and molecular structure of the CD20 marker and its regulation mechanism to improve the understanding of its function within the cell, the effect it exerts as a marker of poor prognosis when expressed in adult patients diagnosed with ALL, and the advantages of being used as a therapeutic target in this pathology.
Cáncer: una breve reseña de una enfermedad furtiva desde tiempos inmemoriales.
(Universidad Internacional SEK, 2023) Chauca Abad, Valeria Estefania; Intriago Baldeón, Damaris P.; Samaniego Villacís, Ana Claudia; Bigoni Ordóñez, Gabriele Davide; Otavalo Anguisaca, Christian Fernando
Molecular iodine inhibits the expression of stemness markers on cancer stem-like cells of established cell lines derived from cervical cancer
(2018) González Valencia, Heriberto; Bigoni Ordóñez, Gabriele Davide; García Carrancá, Alejandro; Aceves, Carmen; Ortiz Sánchez, Elizabeth; Rosendo Chalma, Pedro
Background: Cancer stem cells (CSC) are characterized by deregulated self-renewal, tumorigenicity, metastatic potential, aberrant stemness signaling pathways, resistance to conventional therapy, and the ability to give rise to a progeny of proliferating cells that constitute the bulk of tumors. Targeting CSC will provide novel treatments for cancer. Different investigations have focused on developing complementary approaches that involve natural compounds that decrease chemo-resistance and reduce the side effects of conventional therapies. Since, it has been reported that molecular iodine (I2) exhibits antineoplastic effects and decreases tumor progression in some cancer models, we evaluated the potential effect of I2 on cell cultures enriched in cervical cancer stem-like cells. Methods: HeLa and SiHa cervical cancer cells were treated with 200uM I2 for 24 h. After time, cells were cultured in CSC-conditioned medium (cervospheres) and viability assays were performed. Following, tumorigenic capabilities in cervospheres treated with I2 were evaluated in NOD/SCID mice. HeLa monolayer cells untreated and their respective cervosphere cells treated or untreated with 200 μM of I2 for 24 h were xenotransplanted subcutaneously at different amounts and mice were monitored for at least 2 months. Results: In the present study, monolayer and CSC-enriched cultures (cervospheres) from cervical cancer-derived cell lines, HeLa and SiHa, showed that 200uM I2 supplementation inhibits proliferation of both and decreased their tumorigenic capacity, in vivo. This antineoplastic effect of I2 was accompanied by diminished expression of stemness markers including CD49f, CK17, OCT-4, NANOG, SOX2, and KLF4, as well as increased expression and activation of PPARγ receptors. Conclusions: All this data led us to suggest a clinical potential use of I2 for targeting CSC and improve current treatments against cervical cancer.
Prevalencia de los genotipos del virus del papiloma humano en mujeres de 25 a 65 años
(2020) Minchalo Muñoz, Diana Janneth; Oleas Seminario, Héctor Lincoln; Bigoni Ordóñez, Gabriele Davide
Introduction: The infection caused by the Human Papilloma Virus (HPV) has a high prevalence in sexually active women. It is generally temporary, but when there are some related factors, they can develop cervical cancer. Since the disease develops slowly, detection in early stages has made it possible to reveal the presence of the virus in cells before they can transform and become tumorigenic. The objective of this study was to establish the prevalence of Human Papilloma Virus genotypes in women aged 25 to 65 years in a group of patients from an oncology center in Cuenca 2017-2018. Methods: It is a descriptive, retrospective, analytical study, in which information was collected from the medical records and physical records of the Molecular Biology Laboratory and the SOLCA - Cuenca medical system, SOFTCASE, to establish the prevalence of HPV during the period 2017 - 2018. ODDS Ratio is used to demonstrate association between demographic variables and high-risk HPV versus low-risk HPV serology groups. Results: 594 cases were included, aged between 36 and 40 years, n = 103/594 (17.3%). Marital status married n = 318/594 (53.5%). With parity equal to 2 n = 159/594 (26.8%). Positive HPV cases were 424/594 (71.38%) 95% CI (71.23% to 71.53%), high risk genotypes with 58.01%, probable low risk genotypes with 33.25% and low risk genotypes 8.72%. The prevalence of 50% of the positive population according to genotype is explained by HPV 16, 71, 58, 6 and 31. Of this group, HPV with serology 16, 58 and 31 have a high risk of malignancy. No association was reported between high-risk HPV with any of the demographic variables. Conclusion: The age group with the highest number of positive cases belonged to women between 36 and 40 years of age, with parity equal to 2 and married marital status. The HPV-16 subtype was the most prevalent genotype in the group at high risk of malignancy. The HPV-71 subtype was the second most prevalent genotype with a profile of probable low risk of malignancy.
Microbiota y su relación en el cáncer
(2021) Otavalo Anguisaca, Christian Fernando; Bigoni Ordóñez, Gabriele Davide; Machuca Carpio, Carlos Andrés
The human microbiota is made up of a wide diversity of microorganisms that inhabit our body, playing very important roles in maintaining homeostasis. However, there is more and more evidence that relates the conformational states of the microbiota with the etiology and pathophysiology of different diseases in humans, including cancer. This review details some molecular interactions through which the microbiota affects the pathophysiology of cancer, the role of the virioma and its possible application as a powerful treatment. Finally, understanding the relationship between the microbiota and cancer could give a better understanding of carcinogenesis and the development of new medical strategies and approaches aimed at the prevention, treatment and diagnosis of this disease in a more precise way.
CDH1 and SNAI1 are regulated by E7 from human papillomavirus types 16 and 18
(2020) Patiño Morales, Carlos César; Bigoni Ordóñez, Gabriele Davide; García Carranca, Alejandro; Cano García, Amparo; Rosendo Chalma, Pedro; Antonio Vejar, Verónica
A common characteristic of cancer types associ- ated with viruses is the dysregulated expression of the CDH1 gene, which encodes E-cadherin, in general by activation of DNA methyltransferases (Dnmts). In cervical cancer, E7 protein from high risk human papillomaviruses (HPVs) has been demonstrated to interact with Dnmt1 and histone deacetylase type 1 (HDAC1). The present study proposed that E7 may regulate the expression of CDH1 through two pathways: i) Epigenetic, including DNA methylation; and ii) Epigenetic-independent, including the induction of negative regulators of CDH1 expression, such as Snail family tran- scriptional repressor Snai1 and Snai2. To test this hypothesis, HPV16- and HPV18-positive cell lines were used to determine the methylation pattern of the CDH1 promoter and its expres- sion in association with its negative regulators. Different methylation frequencies were identified in the CDH1 promoter in HeLa (88.24%) compared with SiHa (17.65%) and Ca Ski (0%) cell lines. Significant differences in the expression of SNAI1 were observed between these cell lines, and an inverse association was identified between the expression levels of SNAI1 and CDH1. In addition, suppressing E7 not only increased the expression of CDH1, but notably decreased the expression of SNAI1 and modified the methylation pattern of the CDH1 promoter. These results suggested that the expres- sion of CDH1 was dependent on the expression of SNAI1 and was inversely associated with the expression of E7. The present results indicated that E7 from HPV16/18 regulated the expres- sion of CDH1 by the two following pathways in which Snai1 is involved: i) Hypermethylation of the CDH1 promoter region and increasing expression of SNAI1, as observed in HeLa; and ii) Hypomethylation of the CDH1 promoter region and expres- sion of SNAI1, as observed in SiHa. Therefore, the suppression of CDH1 and expression of SNAI1 may be considered to be biomarkers of metastasis in uterine cervical cancer.