Person:
Ortiz Ulloa, Silvia Johana

Birth Date

1983-01-06

ORCID

0000-0002-7171-7135

Scopus Author ID

55597103900

Afiliación

Universidad de Cuenca, Cuenca, Ecuador
Universidad de Cuenca, Departamento de Biociencias, Cuenca, Ecuador

País

Ecuador

Organizational Units

Organizational Unit

Facultad de Ciencias Químicas

Fundada en 1955 como la Escuela de Química Industrial, la facultad ha sido un pilar fundamental en la formación de profesionales altamente capacitados, comprometidos con el desarrollo de la ciencia, la educación y el bienestar social. La Facultad de Ciencias Químicas pone a consideración su trabajo académico, investigativo y de vinculación con la sociedad, desarrollado a través de la práctica de una docencia de calidad, investigación e innovación en su área de estudio. Desde su oficio de conocimiento se permite contribuir a la sociedad con cuatro carreras: Bioquímica y Farmacia, Ingeniería Química, Ingeniería Ambiental e Ingeniería Industrial. Su carta de presentación en la Academia, la coloca como una dependencia dinámica, donde confluye la solidez de una trayectoria de más de sesenta años. Aquí se trabaja en una continua formación de pregrado y posgrado de la más alta calidad, mediante la mejora continua con la innovación y a la vanguardia de las ciencias químicas.

Job Title

Profesor (T)

Last Name

Ortiz Ulloa

First Name

Silvia Johana

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Now showing 1 - 9 of 9

Revisión sistemática de estudios sobre inocuidad alimentaria en Cuenca, Ecuador, periodo 1981-2017
(2020) Ortiz Ulloa, Silvia Johana; Castro Arteaga, Evelyn Michelle; Ochoa Avilés, Angélica María; Donoso Moscoso, Silvana Patricia
Objetivo: Evaluar la evidencia existente sobre inocuidad alimentaria en el cantón Cuenca, Ecuador. Materiales y métodos: Se realizó una revisión sistemática de los estudios en inocuidad alimentaria (microorganismos, contaminantes y adulterantes) durante el período 1981-2017. Resultados: Inicialmente se identificaron 640 estudios. Luego del análisis de los criterios de inclusión y exclusión, se incluyeron 40 estudios relacionados a evaluaciones microbiológicas (67.5%), pesticidas, parásitos y antibióticos (7.5%), conservantes y metales (10%) y toxinas (15%). Discusión: En los estudios se recalca la necesidad de implementar medidas de higiene y monitoreo, control en la manipulación, transporte y almacenamiento, control de los alimentos frescos cuya calidad no está regida por normativas nacionales, capacitar a los manipuladores y vendedores ambulantes. Esta revisión proporciona una base racional para toma de decisiones y desarrollo de medidas preventivas y correctivas; así como también propone los criterios mínimos de muestreo y análisis para discriminar y comparar estudios en esta problemática.
Two years of school-based intervention program could improve the physical fitness among Ecuadorian adolescents at health risk: Subgroups analysis from a cluster-randomized trial
(2016) Andrade Tenesaca, Dolores Susana; Ochoa Avilés, Angélica María; Donoso Moscoso, Silvana Patricia; Ortiz Ulloa, Silvia Johana
Background Adolescents with overweight and poor physical fitness have an increased likelihood of developing cardiovascular diseases during adulthood. In Ecuador, a health promotion program improved the muscular strength and speed-agility, and reduced the decline of the moderate-to-vigorous physical activity of adolescents after 28 months. We performed a sub-group analysis to assess the differential effect of this intervention in overweight and low-fit adolescents. Methods We performed a cluster-randomized pair matched trial in schools located in Cuenca–Ecuador. In total 20 schools (clusters) were pair matched, and 1440 adolescents of grade 8 and 9 (mean age of 12.3 and 13.3 years respectively) participated in the trial. For the purposes of the subgroup analysis, the adolescents were classified into groups according to their weight status (body mass index) and aerobic capacity (scores in the 20 m shuttle run and FITNESSGRAM standards) at baseline. Primary outcomes included physical fitness (vertical jump, speed shuttle run) and physical activity (proportion of students achieving over 60 min of moderate–to-vigorous physical activity/day). For these primary outcomes, we stratified analysis by weight (underweight, normal BMI and overweight/obese) and fitness (fit and low fitness) groups. Mixed linear regression models were used to assess the intervention effect. Results The prevalence of overweight/obesity, underweight and poor physical fitness was 20.3 %, 5.8 % and 84.8 % respectively. A higher intervention effect was observed for speed shuttle run in overweight (β = −1.85 s, P = 0.04) adolescents compared to underweight (β = −1.66 s, P = 0.5) or normal weight (β = −0.35 s, P = 0.6) peers. The intervention effect on vertical jump was higher in adolescents with poor physical fitness (β = 3.71 cm, P = 0.005) compared to their fit peers (β = 1.28 cm, P = 0.4). The proportion of students achieving over 60 min of moderate-to-vigorous physical activity/day was not significantly different according to weight or fitness status. Conclusion Comprehensive school-based interventions that aim to improve diet and physical activity could improve speed and strength aspects of physical fitness in low-fit and overweight/obese adolescents.
Tabla de composición de alimentos Cuenca, Ecuador
(Universidad de Cuenca, 2018) Ortiz Ulloa, Silvia Johana; Astudillo Rubio, Gabriela Cristina; Donoso Moscoso, Silvana Patricia; Ochoa Avilés, Angélica María; Ortiz Ulloa, Silvia Johana; Donoso Moscoso, Silvana Patricia; Ochoa Avilés, Angélica María
The designations employed and the presentation of this material do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations (FAO) concerning the legal or development status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. The mention of specific companies or products of manufacturers, whether or not these have been patented, does not imply that these have been endorsed or recommended by FAO in preference to others of a similar nature that are not mentioned. The views expressed in this information product are those of the author(s) and do not necessarily reflect the views of FAO.
In vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/Caco-2 cell model
(2023) Van Camp, John Hendrik; Van de Wiele, Tom Richard; Cabezas Teran, Katty Elizabeth; Grootaert, Charlotte; Donoso Moscoso, Silvana Patricia; Ruales Najera, Jeny Cumanda; Van Bockstaele, Filip; Ortiz Ulloa, Silvia Johana
β-carotene is a carotenoid with provitamin A activity and other health benefits, which needs to become bioavailable upon oral intake to exert its biological activity. A better understanding of its behaviour and stability in the gastrointestinal tract and means to increase its bioavailability are highly needed. Using an in vitro gastrointestinal digestion method coupled to an intestinal cell model, we explored the stability, gastrointestinal bioaccessibility and cellular uptake of β-carotene from microparticles containing carotenoid extracts derived from mango by-products. Three types of microparticles were tested: one with the carotenoid extract as such, one with added inulin and one with added fructooligosaccharides. Overall, β-carotene was relatively stable during the in vitro digestion, as total recoveries were above 68 %. Prebiotics in the encapsulating material, especially inulin, enhanced the bioaccessibility of β-carotene almost 2-fold compared to microparticles without prebiotics. Likewise, β-carotene bioaccessibility increased proportionally with bile salt concentrations during digestion. Yet, a bile salts level above 10 mM did not contribute markedly to β-carotene bioaccessibility of prebiotic containing microparticles. Cellular uptake experiments with non-filtered gastrointestinal digests yielded higher absolute levels of β-carotene taken up in the epithelial cells as compared to uptake assays with filtered digests. However, the proportional uptake of β-carotene was higher for filtered digests (24 – 31 %) than for non-filtered digests (2 – 8 %). Matrix-dependent carotenoid uptake was only visible in the unfiltered medium, thereby pointing to possible other cellular transport mechanisms of non-micellarized carotenoids, besides the concentration effect. Regardless of a filtration step, inulin-amended microparticles consistently resulted in a higher β-carotene uptake than regular microparticles or FOS-amended microparticles. In conclusion, encapsulation of carotenoid extracts from mango by-products displayed chemical stability and release of a bioaccessible β-carotene fraction upon gastrointestinal digestion. This indicates the potential of the microparticles to be incorporated into functional foods with provitamin A activity.
12th IFDC 2017 special issue – seasonal variations in nutrient composition of plant-based foods produced at the southern highlands of Ecuador
(2019) Ortiz Ulloa, Silvia Johana; Astudillo Rubio, Gabriela Cristina; Castro Arteaga, Evelyn Michelle; Castro Nube, Cecilia; Astudillo Astudillo, Sonia Cecilia; Donoso Moscoso, Silvana Patricia
Changes in environmental conditions may influence the biosynthesis of several food nutrients. This study aimed to compare macronutrient and mineral composition over several seasons in 25 fresh plant-based foods that are highly consumed and locally produced in the southern Ecuadorian highlands. Samples were collected during the rainy season (October 2015–March 2016) and dry season (April–September 2016) from main local markets and supermarkets. Analyses of composite samples were carried out in triplicate following AOAC methods, determining moisture by desiccation, ash by calcination, total fat by Weibull, total nitrogen by Kjeldahl, total carbohydrates by difference, phosphorus by colorimetry, and minerals (Na, K, Mg, Ca, Fe, Se, Cu and Zn) by atomic absorption spectroscopy. Overall, during the rainy season, significantly higher moisture content was observed (86.7 ± 9.0% vs. 85.9 ± 8.9%, p < 0.001), whereas fat (0.21 ± 0.21% vs. 0.31 ± 0.24%, p = 0.001), Fe (0.79±1.31 vs. 0.61 ± 0.98 mg/100 g, p < 0.001), Ca (50.4±68.0 vs. 23.0 ± 37.3 mg/100 g, p < 0.001), Mg (18.0 ± 11.4 vs. 15.2 ± 10.8 mg/100 g, p < 0.001) and Zn (0.35±0.69 vs. 0.2 ± 0.16 mg/100 g, p = 0.026 were significantly lower. This study demonstrates the influence of the season in the composition of vegetables cultivated in Ecuador. This factor, along with other sources of variability, should be defined, so as to be included in the quality assessments of representative food composition data. © 2019 Elsevier Inc.
Control microbiológico de alimentos en la vía pública en Cuenca, Ecuador
(2023) Ortiz Ulloa, Silvia Johana; León Vizñay, Jéssica Andrea; Donoso Moscoso, Silvana Patricia; Astudillo Rubio, Gabriela Cristina; Astudillo Neira, Diana Ligia de Lourdes
Street-vending trade in ready-to-eat foods has grown exponentially, worldwide. However, the lack of optimal conditions for the preparation and sale of these foods can affect their safety. The objective of this study was to evaluate the microbiological quality of 19 types of foods (n= 417), with and without heat treatment, sold in public spaces in Cuenca, Ecuador. According to the food group, mesophilic aerobes, coliforms/Escherichia coli, molds and yeasts, Staphylococcus aureus, Salmonella, Listeria spp., Clostridium perfringens, and Vibrio parahaemolyticus were examined. For microorganisms identification and counting, national regulations were applied and, in cases of absence, regulations from other countries were adopted. As a result, 55,4% of the samples were considered inadequate for consumption. S. aureus was the most prevalent presumptive pathogenic microorganism (81.7%). Non-compliance with regulations was significantly higher among foods without heat treatment (54.1%) compared to those heat treated (24%) and those that combine ingredients with and without treatment (21.9%) (p[removed]
Multiple mycotoxin exposure of infants and young children via breastfeeding and complementary/weaning foods consumption in Ecuadorian highlands
(2018) Ortiz Ulloa, Silvia Johana; Donoso Moscoso, Silvana Patricia; Huybregts, Lieven Fernand
The dietary exposure to mycotoxins in Ecuadorian children aged 0–23 months (320 rural and 603 urban) was evaluated based on the intake of breast milk and staple cereals used as complementary/weaning foods. A probabilistic distribution approach by first order Monte Carlo simulation was adopted to assess the locally occurring mycotoxins (aflatoxins M1 and B1 in breast milk, ochratoxin A and deoxynivalenol in wheat noodles and oat flakes, and HT-2 toxin in polished rice). Overall, exposure was modest but higher for rural children due to their monotonous diet. Aflatoxin exposure by breast milk intake were of health concern in both areas (Margin of Exposure and Combined Margin of Exposure Index<10,000). Mycotoxin exposure by staple cereals intake was considered tolerable across feeding stages for individual mycotoxin-cereal combination (Hazard Quotient<1) and combined exposure (Hazard Index<1). The major exposure was to HT-2 toxin by rice intake at complementary feeding (15% rural and 4% urban above TDI) and at weaning stage (26% rural and 6% urban above TDI). Since the usual Ecuadorian diet is based on the same staple cereals, risk management actions could lead to a better protection of young children and also ensure higher safety of the recommended breastfeeding practices by protecting nursing mothers. © 2018 Elsevier Ltd
Children’s and adolescents’ rising animal-source food intakes in 1990–2018 were impacted by age, region, parental education and urbanicity
(2023) Ochoa Avilés, Angélica María; Ortiz Ulloa, Silvia Johana
Animal-source foods (ASF) provide nutrition for children and adolescents’ physical and cognitive development. Here, we use data from the Global Dietary Database and Bayesian hierarchical models to quantify global, regional and national ASF intakes between 1990 and 2018 by age group across 185 countries, representing 93% of the world’s child population. Mean ASF intake was 1.9 servings per day, representing 16% of children consuming at least three daily servings. Intake was similar between boys and girls, but higher among urban children with educated parents. Consumption varied by age from 0.6 at [removed]
Sugar-sweetened beverage intakes among adults between 1990 and 2018 in 185 countries
(2023) Ortiz Ulloa, Silvia Johana; Ochoa Avilés, Angélica María
Sugar-sweetened beverages (SSBs) are associated with cardiometabolic diseases and social inequities. For most nations, recent estimates and trends of intake are not available; nor variation by education or urbanicity. We investigated SSB intakes among adults between 1990 and 2018 in 185 countries, stratified subnationally by age, sex, education, and rural/urban residence, using data from the Global Dietary Database. In 2018, mean global SSB intake was 2.7 (8 oz = 248 grams) servings/week (95% UI 2.5-2.9) (range: 0.7 (0.5-1.1) in South Asia to 7.8 (7.1-8.6) in Latin America/Caribbean). Intakes were higher in male vs. female, younger vs. older, more vs. less educated, and urban vs. rural adults. Variations by education and urbanicity were largest in Sub-Saharan Africa. Between 1990 and 2018, SSB intakes increased by +0.37 (+0.29, +0.47), with the largest increase in Sub-Saharan Africa. These findings inform intervention, surveillance, and policy actions worldwide, highlighting the growing problem of SSBs for public health in Sub-Saharan Africa