Person:
Samaniego Campoverde, Jorge Xavier

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Birth Date

1993-03-01

ORCID

0000-0002-1599-041X

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58627486100

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Universidad de Cuenca, Cuenca, Ecuador
Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Laboratorio de Biotecnología de La Reproducción Animal, Cuenca, Ecuador

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Ecuador

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Facultad de Ciencias Agropecuarias
El 21 de abril de 1982, por resolución del Honorable Consejo Universitario se establece la Facultad de Ciencias Agropecuarias, de la Universidad de Cuenca. La Facultad de Ciencias Agropecuarias es una institución formadora de talento humano en el área agronómica, a través de una educación de calidad, centrada en la investigación y vinculación con la colectividad. Los futuros profesionales médicos veterinarios zootecnistas e ingenieros agrónomos, durante su permanencia en las aulas y estudio de campo, desarrollan conocimientos científicos-tecnológicos, competencias y destrezas en procesos de producción agropecuaria. Se los prepara con el fin de preservar la salud de los animales y recursos naturales, fomentando la seguridad alimentaria, respetando el medio ambiente dentro del marco del Buen Vivir, englobado en las necesidades de la región y el país.

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Samaniego Campoverde

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Jorge Xavier

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    Publication
    La suplementación de jalea real a diluyente de base sintética y no sintética ayuda a mantener la motilidad y cinética de esperma de toro refrigerado
    (Universidad de Cuenca, 2022) Galarza Lucero, Diego Andrés; Guanga Tenempaguay, Jacqueline; Lucero Zhumi, Juan Ismael; Samaniego Campoverde, Jorge Xavier; Galarza Lucero, Diego Andrés; Galarza Lucero, Diego Andrés
    Royal Jelly (RJ) is a substantial nutrient produced by the hypopharynx and mandibular glands of worker bees from 4 to 11 days of age, and its composition is mainly based on water (60–70%), protein (27–41% ) and carbohydrates (30%), lipids (8–19%), vitamins (A, B, C, E), salts, and amino acids. Due to its composition, JR has been successfully used in sperm cryopreservation at concentrations from 0.2 to 1% (w/v) supplemented with synthetic base extenders (eg TRIS or TALP). Its effect has been evidenced in the increase of motility, viability, functionality of the plasmatic membrane and fertilizing capacity of fresh and cryopreserved ram sperm. Conventional freezing requires the use of penetrating cryoprotectants (eg glycerol) to prevent damage caused by ice crystal formation, however, vitrification requires high concentrations (0.1 to 6 M) of non-penetrating cryoprotectants (eg sucrose or trehalose) and rapid cooling, passing rapidly to the glass transition without forming ice crystals. This research evaluated the effect of JR (0.2%) supplemented to freezing (TCG-YH + 5% glycerol) and vitrification (TCG-YH + 100 mM sucrose) media on cryosurvival of bull sperm.
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    Publication
    Optimization of cryopreservation of arabian stallion sperm using dimethylformamide, glycerol, and different freezing protocols
    (Wiley-Blackwell, 2022) Serpa Carangui, Erika Margoth; Samaniego Campoverde, Jorge Xavier; Galarza Lucero, Diego Andrés; Soria Parra, Manuel Elías; Ochoa Guillén, Juan Diego; Álvarez Palacios, David Alejandro; De la Cruz Tandazo, Francisco Javier; Galarza Lucero, Diego Andrés
    This study evaluated the effect of penetrating cryoprotectant agents (CPA) and the cryosurvival of three freezing protocols on the kinematics and integrity of membranes of frozen-thawed stallion sperm. Twenty-four ejaculates of four adult Arabian horses were collected in six weekly sessions (six ejaculates/horse). Each ejaculate was divided into two aliquots. With the first aliquot, three CPA treatments were conformed: 5% glycerol (GLY), 5% dimethylformamide (DMF), and 3%–3% DMF–GLY combination, and the sperm samples were frozen exposing them to liquid nitrogen (LN2) vapors. The second aliquot was diluted with freezing medium plus 5% DMF and the sperm samples were frozen in three freezing protocols: (P1) Styrofoam cryo-box (30 × 29 × 31 cm of length, width, and height, respectively) with two ramps (at 17 and 7 cm above LN2); (P2) freezing unit® (Minitüb, Germany); and (P3) programmable TK 4000-freezer® (Compacta, Brazil). The DMF-GLY combination and DMF yielded higher (p<.05) post-thaw values than the GLY regarding the motility (SM: 54.2±2.25 and 50.2±1.80 vs. 41.4±2.35%, respectively), curvilinear velocity (VCL: 58.0±1.71 and 54.0±1.58 vs. 42.3±1.60 µm/s), and the proportion of sperm with intact plasma and intact acrosome (IPIA: 58.0±1.11 and 52.6±0.99 vs. 42.5±1.07%). Furthermore, the P1 protocol produced a similar (p>.05) post-thaw SM, VCL, and IPIA than the other protocols. Indeed, the P1 and P3 protocols yielded lower proportion (p<.05) of sperm with damaged plasma and damaged acrosome than the P2 protocol after thawing (3.7±0.18 and 3.1±0.18 vs. 6.1±0.44%, respectively). In conclusion, the addition of DMF or combined with GLY to freezing medium, and the freezing with Styrofoam cryo-box with two ramps increase the cryosurvival of Arabian stallion spermatozoa.
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    Publication
    Cryopreservation of Domestic and Wild Animal Spermatozoa: Update of Knowledge
    (Intechopen, 2023) Samaniego Campoverde, Jorge Xavier; Galarza Lucero, Diego Andrés; Méndez Álvarez, María Silvana; Duma Pauta, José Mauricio
    Current sperm cryopreservation protocols for domestic and wild mammals aim to minimize the cryogenic damage caused by cell dehydration, ice formation, and osmotic stress. The optimization of sperm cryopreservation include the use of different synthetic and nonsynthetic-based extenders supplemented with additives (e.g., egg yolk, coconut water, etc.) and antioxidants (e.g., melatonin, L-carnitine, caffeine, resveratrol, etc.) that protect the plasmalemma, acrosome, and mitochondria against the detrimental effects caused by the cryopreservation process. Furthermore, the use of penetrating (e.g., glycerol, ethylene glycol, dimethylformamide, etc.) and nonpenetrating (e.g., sucrose and trehalose) cryoprotectant agents (CPAs) or their combination should be investigated to protect sperm during the freezing process in slow and ultra-rapid freezing procedures. Finally, new cryopreservation protocols should focus on freezing curves and initial cooling rates that allow optimal dehydration during freezing and adequate hydration during thawing. The suitable interaction of all these factors will allow a sperm subpopulation to survive cryopreservation with integrity and fertilizing capacity, contributing to the improvement of the efficiency of genetic resource management and the development of germplasm banks that support the preservation of genetic diversity in domestic and wild animals.