Person: Bustamante Ordóñez, Jorge Gualberto
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0000-0002-2422-4338
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59236300500
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Universidad de Cuenca, Cuenca, Ecuador
Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador
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Ecuador
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Facultad de Ciencias Agropecuarias
El 21 de abril de 1982, por resolución del Honorable Consejo Universitario se establece la Facultad de Ciencias Agropecuarias, de la Universidad de Cuenca. La Facultad de Ciencias Agropecuarias es una institución formadora de talento humano en el área agronómica, a través de una educación de calidad, centrada en la investigación y vinculación con la colectividad. Los futuros profesionales médicos veterinarios zootecnistas e ingenieros agrónomos, durante su permanencia en las aulas y estudio de campo, desarrollan conocimientos científicos-tecnológicos, competencias y destrezas en procesos de producción agropecuaria. Se los prepara con el fin de preservar la salud de los animales y recursos naturales, fomentando la seguridad alimentaria, respetando el medio ambiente dentro del marco del Buen Vivir, englobado en las necesidades de la región y el país.
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Bustamante Ordóñez
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Jorge Gualberto
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Publication Calidad–inocuidad de la leche cruda de vaca que ingresa a centros de acopio de la provincia Cañar–Ecuador, en el contexto de las normativas latinoamericanas(2023) Lupercio Novillo, Rosa Lucía; Macancela Herrera, Daniel Alberto; Vintimilla Rojas, Andrea Elizabeth; Andrade Guzmán, Omar Santiago; Abad Quevedo, Vanessa Lucia; Agurto Granda, Diego Alonso; López Espinoza, Mateo Damián; Bustamante Ordóñez, Jorge GualbertoThe purpose of this study was to analyze physical–chemical and microbiological parameters of raw cow’s milk, in order to evaluate its quality and safety in relation to Ecuadorian regulations (INEN 009–2012), and to make a comparison with the regulations of the other Countries from Latin America such as Perú, Colombia, Venezuela, México and Argentina. Upon entering 28 collection centers in the Province of Cañar, 203 samples were taken from 6,124 producers. The analysis of: temperature, density, and titratable acidity were performed in situ, while fat, Total Solids (TS), Non – Fat Solids (NFS), protein (P), freezing point (FP), Somatic Cell Count (SCC), Total Bacteria Count (TBC), mesophilic Aerobes and Enterobacteria. They were carried out in the laboratories of AGROCALIDAD and the University of Cuenca. It was observed that in the variables: density (15°C), titratable acidity, fat, TS, NFS, P, FP (°C), the 87.5 to 100 % of samples comply with the Ecuadorian regulations and the regulations of the Countries analyzed; Colombian and Venezuelan regulations have more demanding of reference values for density, protein, titratable acidity and NFS, respectively. México and Argentina do not refer to TS and Perú doesn’t consider P. However, the results in microbiology are discouraging regarding the count of mesophilic aerobics, as only 8 % of the samples analyzed comply with the regulations of all Countries. The microbiological load is high, despite the fact that no regulation analyzed indicate maximum for TBC, an average of 24,000 × 103 colony forming units per millimeter (CFU·mL–1) was found. As a reference, some regulations in the USA set, despite a maximum of 300 × 103 CFU·mL–1 of TBC in blended milk. Regarding SCC, according to Argentine regulations, only 31 % of the samples would be within the parameters. This work provides knowledge of the current status of the quality–safety of raw milk in an important dairy area, and analyzes these results in the context of international regulations, serving to focus on continuous improvement with a view to export.Publication Relación del número de partos, edad y la morfoestructura del genotipo Holstein en la Provincia del Azuay(2017) Ortega Herrera, Vanessa Viviana; Pesántez Carmona, José Luis; Pesántez Campoverde, Manuel Teodoro; Guevara Viera, Raúl Victorino; Guevara Viera, Guillermo Emilio; Dután Sanango, Jorge Bolívar; Ayala Rodríguez, Lorena Estefania; Murillo Apolo, Yury Agapito; Calle Ortiz, Guido Rigoberto; Samaniego Campoverde, Jorge Xavier; Bustamante Ordóñez, Jorge Gualberto; Vanegas Cabrera, Raúl Alejandro; Serpa García, Víctor Guillermo; Rodas Carpio, Ermes Ramiro; Nieto Escandón, Pedro Emilio; Vázquez Mosquera, Juan Mesías; Andrade Guzmán, Omar SantiagoPublication Eficacia de la prueba Elisa en muestras de leche para la vigilancia epidemiológica de la brucelosis bovina(Archivos Latinoamericanos de Producción Animal, 2022) Dután Sanango, Jorge Bolívar; Lupercio Novillo, Rosa Lucía; Andrade Guzmán, Omar Santiago; López Espinoza, Mateo Damián; Vintimilla Rojas, Andrea Elizabeth; Rivera Pirela, Sergio Emiro; Bustamante Ordóñez, Jorge Gualberto; Guevara Viera, Guillermo Null; Andrade Guzmán, Omar SantiagoBovine brucellosis is a worldwide zoonotic disease capable of infecting cattle for long periods of time. Some animals are asymptomatic, thus maintaining the disease in the herd. Cattle become infected after ingestion of contaminated milk, feed, water; also by contact with infected animals, uterine secretions or aborted fetuses and by vertical transmission. Little is known about the epidemiological situation of brucellosis in dairy herds in the province of Azuay. An initial step for the formulation of control and eradication programs would be the implementation of a surveillance system that allows characterizing the state of infection in dairy areas, and thus early detection of the entry of the disease into free herds. The purpose of this study was to evaluate the efficacy of the indirect ELISA test (IDEXX, Brucella tank milk) in milk pools as a tool to identify herds that have not been in contact with the disease, allowing the characterization of areas free of brucellosis.Publication Parámetros de variabilidad genética de bovinos certificados de la raza holstein del cantón Cuenca(2020) Méndez Álvarez, María Silvana; Soria Parra, Manuel Elías; Palacios Ordóñez, Teofilo Estuardo; Andrade Guzmán, Omar Santiago; Bustamante Ordóñez, Jorge Gualberto; Pesántez Pacheco, José Luis; Vázquez Mosquera, Juan Mesías; Guevara Viera, Guillermo Emilio; Vallecillo Maza, Antonio JavierHolstein cattle account for most of the dairy production in Ecuador. However, there are no data on genetic parameters that describe the characteristics of the country’s dairy herds. Therefore, the objective of this study was to analyze the genetic variability of registered Holstein cattle in Cuenca district, Ecuador. For this, the microsatellite markers CSRM60, INRA083, CSSM66, ETH3, HEL9, TGLA53, BM1818, LSTS006, BM2113, ETH225, TGLA122, ETH10, TGLA227, INRA032 and SPS115 were analyzed in 52 bovines, according to the guidelines on genetic characterization of domestic animal species suggested by FAO. It was found an average of 8.4 ± 1.88 alleles and a frequency of 0.08 ± 0.056 of null alleles. The observed and expected heterozygosity values reached 0.68 ± 0.13 and 0.82 ± 0.07, respectively. The polymorphic information content ranged between 0.70 and 0.88, while the inbreeding coefficient varied between -0.38 and 0.45. Of the 15 loci analyzed, only CSSM66 had a Hardy-Weinberg equilibrium and 4 of them showed a high level of Inbreeding. It is concluded that the loci studied show high genetic variability and polymorphism. This provides ample information for paternity and identity studies. However, according to the consanguinity level, high genetic diver-sity should be considered when selecting bulls for artificial insemination programs.Publication Características génicas de las poblaciones de bovinos criollos americanos en base a microsatélites(2019) Guevara Viera, Guillermo Emilio; Vallecillo Maza, Antonio Javier; Méndez Álvarez, María Silvana; Soria Parra, Manuel Elías; Palacios Ordóñez, Teofilo Estuardo; Andrade Guzmán, Omar Santiago; Bustamante Ordóñez, Jorge Gualberto; Pesántez Pacheco, José Luis; Vázquez Mosquera, Juan MesíasThe objective of this study is to analyze the major gene parameters found in different genotypes of cattle of Iberian origin in the Americas. The work was based on the review of different results of the genetic markers analyzed in blood of Creole cattle incountries of North America until cattle in Patagonia in the southern part of South America. Presents results of the markers most used and recommended for ca ttle. Population genetic parameters analyzed were: total number of alleles (Na), by microsatellite marker allele 189 frequencies), the factors observed (Ho) and expected (He), the coefficients of inbreeding (Fis), polymorphic information content) CIP) and compliance with the assumption of Hardy-Weinberg equilibrium. The percentage of variance between populations and the (Fst) is also reported. 20 papers on microsatellites in cattle, analysed 19 of them with informa tion from Creoles of America. Highlights a number of alleles locus of 4 to 13, higher by more than 0.6 heterozygosity and polymorphic information content high. Creole ca ttle have very little variation among their populations, the distance that separates them is not very marked, but they have a Kingdom with many alleles at the loci analyzed and their heterozygosity and polymorphism is high of them with information