Ingeniería Agronómica-Pregrado

Permanent URI for this collectionhttps://dspace-test.ucuenca.edu.ec/handle/123456789/322

Browse

Browsing Ingeniería Agronómica-Pregrado by Subject "Ácido salicílico"

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Efecto del ácido salicílico en la inducción de la resistencia en tomate de árbol (Solanum betaceum) a la antracnosis y caracterización molecular
    (Universidad de Cuenca, 2021-01-07) Cabrera Cabrera, Rolendio Anibal; Castro Quezada, Patricio Salvador
    The tree tomato (Solanum betaceum) is a species of Andean origin, which has great potential due to its organoleptic characteristics. However, it is very susceptible to the presence of anthracnose whose causal agent belongs to the complex Colletotrichum acutatum. This research was conducted in the field and laboratory. In the field, the effect of salicylic acid (AS) in tree tomato was evaluated against anthracnose. To fulfill this objective, the effect of salicylic acid was evaluated in three concentrations (0.1, 0.2, and 0.4 mM). The application was made directly in the crop. For this, a completely randomized block design with 10 repetitions and a control was used. It was also molecularly characterized 16 strains of Colletotrichum acutatum complex from Azuay and Loja provinces through multilocus analysis by PCR, based on primers defined in actin genes, β-tubulin, and glyceraldehyde 3-phosphate dehydrogenase. The following results were obtained for the effect of AS in resistance induction: 0.2 mM concentration showed significant results when anthracnose severity in fruits was reduced; 0.1 mM concentration had significant effects when anthracnose severity in leaves was reduced in front of control plants and anthracnose incidence in leaves. Besides, the 0.4 mM concentration showed a higher leaf and fruit shedding with contaminated tissue as a defense mechanism against the disease compared to the control. Regarding the molecular analysis by multilocus, it was demonstrated that the species responsible for anthracnose in tree tomato is Colletotrichum tamarilloi, which belongs to the Colletotrichum acutatum complex.
  • Thumbnail Image
    Item
    Evaluación in vitro de biofungicidas y ácido salicílico frente a fungicidas químicos para el control de antracnosis en tomate de árbol
    (Universidad de Cuenca, 2023-05-30) Torres Gómez, María José; Castro Quezada, Patricio Salvador
    Anthracnose known as "chicken eye", caused by the phytopathogenic fungus Colletotrichum sp, which is widely distributed in production areas, is the most important disease, since the pathogen not only attacks the fruit at any phenological stage but also the leaves, branches and inflorescences, thus reducing the productive life of the tree by up to 50%. The aim of the present investigation was to evaluate, under in vitro conditions, biofungicides and salicylic acid against chemical products for the control of anthracnose in tree tomato. Samples were taken from 12 farms distributed in the provinces of Azuay, Loja and Cañar. For the collection, the fruits presented the characteristics of the disease as black lesions, accompanied by masses of pink conidia. These samples were subjected to isolation and purification for inhibition tests. The treatments evaluated were salicylic acid 0.4mM, Bacillus subtilis 5ml/L, Bacillus pumilus 5ml/L, pyraclostrobin 13.3% + epoxiconazole 5% 1ml/L, captan 1g/L, difenoconazole 0.75ml/L, and iprodione 1ml/L plus a control treatment. At the in vitro level, salicylic acid inhibited mycelial growth of Colletotrichum sp. from samples collected in the provinces of Azuay, Loja and Cañar, while the T5 treatment (captan) inhibited mycelial growth in samples from Azuay and Loja in the localities of Don Julo, Urdaneta and Saraguro.