Browsing by Author "Taboada Pico, Juan Wualverto"

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Comparación de camadas de cobayas (Cavia porcelus) de genotipos ecuatorianos y la línea mejorada Perú
(2019) Quizhpi Guamán, José Narciso; Nieto Escandón, Pedro Emilio; Taboada Pico, Juan Wualverto; Cedillo Ramón, Johanna Elizabeth; Rosales Jaramillo, Cornelio Alejandro; Guevara Viera, Guillermo Emilio
Background: The danger of disappearance of the different unprotected genotypes in the Andean highlands of Ecuador forces us to study and propose conservation measures to maintain that biological richness that has enormous prospects for the feeding of humanity. The objective of the study was to compare the sizes and weights of the litters until the weaning of native Ecuadorian guinea pigs from the provinces of Azuay, Cañar and the improved line of Peru. Methods: The study was carried out in the experimental farm of Irquis of the Faculty of Agropecific Sciences of the University of Cuenca. The database was constructed with the records of breeders prospective among the farmers of the provinces of Azuay and Cañar, taking as a reference the phenotypic characteristics. Results: No significant differences were proved for the month of parturition or for the number of births or the genotype in the size of the litter at weaning. The Azuay did not differ from Peru in the size of the litter at birth (3.1 and 3.2 respectively). This one exceeded in the weight of the litter at birth and at weaning with 432 and 837 g at 313 and 570 g of Azuay, which does not differ from the Cañar genotype. Conclusions: The Creole genotypes of Azuay and Cañar showed inferiority in the traits related to birth weight and mortality compared to Peru. The Azuay does not differ from Peru or in litter size at birth or at weaning, this genotype manifested a potential that can be developed.
Comparación de la efectividad quirúrgica y posquirúrgica de tres técnicas de orquiectomía canina utilizadas en campañas de esterilización masiva en Cuenca
(2018) Dután Llivisupa, Jennifer Priscila; Taboada Pico, Juan Wualverto
In the present study, 36 male dogs were evaluated, intervened through the massive sterilization project carried out by the University of Cuenca in agreement with the Municipal Autonomous Decentralized Government of the canton of Cuenca. Twelve animals were analyzed by surgical technique and 3 techniques were evaluated: Technique A pre-scrotal orchiectomy, Technique B, traditional scrotal orchiectomy and Technique C, modified scrotal orchiectomy. In the intra-surgical period, the following were taken into account: total times of each technique, amount of blood in the surgical time, surgical costs / kg / dog; in the postoperative period, the following were evaluated: degree of existing pain, recovery in terms of healing, presence of infections, dehiscence of points, loss of weight of the animal, existence of deaths and application of prescription and indications by the owners. These data were obtained on days 2, 8 and 15 after surgery, with them it was determined that there are significant differences between treatments only in the time between techniques in which the mean was 5.46 ± 0.23; 3.18 ± 0.419; 2.25 ± 0.210 minutes, for techniques A, B and C respectively. In the amount of bleeding, an average of 0.56 ± 0.076 was obtained; 0.67 ± 0.098; 0.52 ± 0.102 grams, for each technique, in terms of costs, the means obtained were 19.67 ± 0.874; 19.60 ± 0.932; 18.27 ± 0.833 dollars. In the postoperative evaluation, the most convenient technique considering lower pain, minor complications and better healing turned out to be technique A.
Comparison between ecuadoran genotypes of cavy litters (cavia porcellus) and cavies bBred in Peru
(2019) Rosales Jaramillo, Cornelio Alejandro; Cedillo Ramon, Johanna Elizabeth; Taboada Pico, Juan Wualverto; Nieto Escandón, Pedro Emilio; Quizhpi Guamán, José Narciso; Guevara Viera, Guillermo Emilio
Background: The threat of extinction posed on different unprotected genotypes from the Andean Ranges of Ecuador is calling for new studies and preservation measures to keep the biological diversity of the area, which has enormous perspectives for human nutrition. The aim of this paper was to compare the sizes and weights up to the weaning stage of native Ecuadoran cavy litters in Azuay province, with the Peruvian-bred line. Methods: The study took place on Irquis experimental farm, belonging to the Faculty of Agricultural Sciences, University of Cuenca, Ecuador. The database was based on the records of breeding animals screened among farmers from Azuay and Cañar, and it included their phenotypic traits. Results: No significant differences were observed in the month of parturition, or the number of parturitions, or the genotype regarding the size of the litter at weaning. The data from Azuay and Peru were similar in terms of litter size at birth (3.1 and 3.2, respectively). However, it was higher regarding litter weight at birth and weaning (432 and 837 g, compared to the 313 and 570 g from Azuay, but no different from Cañar). Conclusions: The native genotypes from Azuay and Cañar were inferior to the Peruvian genotype, in terms of birth weight and weaning weight. Azuay was no different from Peru, according to litter size at birth or weaning. The potential observed in this genotype should be further developed.
Cryopreservation of dog epididymal spermatozoa by conventional freezing or ultra-rapid freezing with nonpermeable cryoprotectant
(2021) Landi Loja, Blanca Gabriela; Santiago Moreno, Julián; Mejia Jara, Edisson Leonardo; Galarza Lucero, Diego Andrés; Castaño, Cristina; Sánchez Calabuig, María Jesús; Taboada Pico, Juan Wualverto; Soria Parra, Manuel Elías; Méndez Álvarez, María Silvana; Samaniego Campoverde, Jorge Xavier
This study was aimed to assess the effectiveness of two methods for cryopreservation of dog epididymal spermatozoa, one by conventional freezing (CF) with shortening both equilibration and cooling times, and the other by ultra-rapid freezing (URF) with nonpermeable cryoprotectant. Sixty epididymides were recovered from thirty orchiectomized adult dogs and the sperm samples were retrieved by retrograde flushing using TCG-EY (tris, citric acid, glucose + 20% egg yolk) extender and then 20 pools were conformed. Each pool was divided into 2 aliquots and then cryopreserved by CF and URF methods respectively. The CF method maintained the cooled-pool samples for 2h (1h without and 1h with 5% glycerol) and then were frozen by liquid nitrogen (LN2) vapors for 2 min. The URF method cryopreserved the cooled-pool samples using TCG-EY+250 mM sucrose, equilibrating during 30 min (5 ◦C) and submerging 30-μL drops directly in LN2. The results showed that the URF method produced a lower percentage of total and progressive motilities and acrosome integrity (P < 0.05) than the CF method. However, the kinetic variables (curvilinear and straight-line velocities, straightness, linearity, wobble, amplitude of lateral head displacement, and beat-cross frequency) and plasma membrane integrity did not differ (P > 0.05) between both cryopreservation methods. Unlike the URF method, the width, area and perimeter of sperm head were reduced after the CF method (P < 0.05). In conclusion, despite the low motility achieved after the ultra-rapid freezing method, the similar values of kinetic, viability and head morphometric dimensions to those obtained after conventional freezing, suggest that ultra-rapid freezing with sucrose may be a useful alternative for the cryopreservation of canine epididymal sperm.
Desarrollo agroindustrial: un impulso para la producción animal en industrialización
(Universidad Nacional de Chimborazo. UNACH, 2024) Rosales Jaramillo, Cornelio Alejandro; Taboada Pico, Juan Wualverto; Once Carabajo, Veronica Elizabeth; Ayala Guanga, Luis Eduardo
Diagnóstico de la degeneración valvular mitral mixomatosa en perros por medio de ecocardiografía
(Universidad de Cuenca, 2021-10-13) Salgado Morales, Josué Santiago; Taboada Pico, Juan Wualverto
Myxomatous Mitral Valve Degeneration (MMVD) is one of the main valve diseases causing mitral regurgitation. This condition is most common in dogs with a 75% presentation with high mortality. The aim of this research was to implement the echocardiography technique in the diagnosis of MMVD according to age, sex and rate of disease progression. 200 previously clinically selected dogs with heart desease were required to undergo an echocardiographic examination, by assessing the morphometry of cardiac structures and blood flow to determine the diagnosis of the disease with the established variables. As a statistical method, cross-tabulations were established and the Pearson's Chi-square test was applied to determine significances between the different groups and the Odds Ratio was determined to establish the frequency of presentation of the disease. It was concluded that of the total number of patients evaluated, the MMVD represents 54,5%. In the univariate analysis for sex and for the progression index, there was no statistical significance(p> 0,05), while for age there wasstatistical significance (p <0,05). In the multivariate analysis of sex with age,statistical significance (p <0,05) was established in Senior Males with a relative value of 68,5% in the presentation of MMVD and an estimate of the riskof presenting the disease of 6,53 times more; while in Senior Females thestatistical significance (p <0,05) was also present with a relative value of 62,9% in the presentation of MMVD and an estimate of the risk of presenting the disease of 5,4 times more.
Efecto de bioaditivos en indicadores bioproductivos de cobayas (Cavia porcellus) nulíparas y sus crías
(2022) Miranda Yuquilema, Jose Efrain
Objective. evaluate the effect of bioadditives on the bioproductive indicators of nulliparous guinea pigs (Cavia porcellus) and their offspring. Materials and methods. A total of 40 improved nulliparous guinea pigs, 125 days old, 1450 g live weight, were used, divided into four groups of 10 guinea pigs each. T1, Control (basal diet without additive); T2, bioadditive with Lactobacillus acidophilus. T3, bioadditive with Kluyveromyces fragilis and T4, bioadditive with L. acidophillus and K. fragilis. A completely randomized design was used where weight gain during pregnancy, age at first delivery, percentage of fertility, conception index, health, and values of hemoglobin, hematocrit and mean corpuscular volume were evaluated. Results. In animals that consumed bioadditive T4, weight gain during pregnancy was greater (p<0.05); the age at first delivery was reduced (p<0.05); the fertility percentage and the conception index were better (p<0.05) and the occurrence of diarrhea was lower (p<0.05) in the T4 group. Hematological values improved in all groups that consumed biopreparations. Conclusions. The additives with L. acidophilus and K. fragilis improved the productive and reproductive indicators in primiparous guinea pigs. In addition, it intervenes in the improvement of health and hematological values.
Effect of Agroindustrial Waste Substrate Fermented with Lactic Acid Bacteria and Yeast on Changes in the Gut Microbiota of Guinea Pigs
(2024) Taboada Pico, Juan Wualverto
The aim of the study was to evaluate the impact of probiotics obtained from an agroindustrial waste substrate fermented with lactic acid bacteria and/or yeasts on the health and changes in the microbiota of the digestive tract of guinea pigs. Eighty male guinea pigs, Kuri breed, 30 days old and 250 g live weight, were randomly selected and divided into four groups of 20 animals each: T0, control; T1, Lactobacillus acidophilus and L. bulgaricus; T2, Saccharomyces cerevisiae and Kluyveromyces fragilis; and T3. L. acidophilus, L. bulgariccus, S. cerevisiae and K. fragilis. T1, T2 and T3 contained molasses-vinasse substrate in their base, the dose administered was 1.00 mL/animal orally every 3 days. The indicators evaluated were weight gain, occurrence of diarrhea and mortality, macroscopic lesions in the digestive tract organs and changes in the microbiota of the stomach, caecum, small and large intestine. Treatments T1, T2 and T3 improved weight gain (p < 0.05) and reduced the presence of guinea pigs with diarrhea (p < 0.05) and there was no mortality; animals in the control group presented a greater amount (p < 0.05) of macroscopic lesions in the digestive tract organs; in the T1, T2 and T3 groups there was an improvement in the natural microbiota. It is concluded that the inclusion of a microbial additive in young guinea pigs improves intestinal health and consequently improves weight gain, reduces diarrhea and deaths and normalizes the natural microbiota of the gastrointestinal tract.
Effect of resveratrol supplementation in conventional slow and ultra-rapid freezing media on the quality and fertility of bull sperm
(2024) Aguirre Narea, Brian Oswaldo; Amón Togra, Nicole Alexandra; Jaramillo López, Jennifer Alexandra; Galarza Lucero, Diego Andrés; Duma Pauta, José Mauricio; Samaniego Campoverde, Jorge Xavier; Taboada Pico, Juan Wualverto; Campoverde Guailacela, Byron Alexander
The study investigated the impact of resveratrol (RES) on bull sperm cryopreservation employing conventional slow (CS) and ultra-rapid (UR) freezing methods on sperm quality and in vitro fertility. Twenty-four ejaculates from four bulls were divided into four groups based on the cryopreservation method and RES addition: CS-RES (n = 80), CS-Co (n = 80), UR-RES (n = 24), and UR-Co (n = 24). The CS freezing involved exposing sperm straws with 5% glycerol to liquid nitrogen (LN2) vapors, while UR freezing submerged sperm drops with 100 mM sucrose directly into LN2. Overall, sperm kinematic parameters and integrity of plasma and acrosome membranes significantly decreased (P < 0.001) after cryopreservation. Post-thaw values of motilities (total [TM] and progressive [PSM]), velocities (curvilinear and straight-line), beat cross frequency (BCF), and sperm with intact plasma membrane/intact acrosome (PI-/PNA-) were higher (P < 0.05) with CS-RES and CS-Co treatments compared to UR-RES and UR-Co treatments. CS-RES treatment resulted in greater percentages (P < 0.05) of TM, PSM, PI-/PNA-, and fertility (blastocyst rate) than their control, CS-Co; while UR-RES showed higher BCF values (P < 0.05) than its control, UR-Co. Additionally, UR-RES treatment exhibited lower oxidative stress percentages than UR-Co (P < 0.05). This study presents the following conclusions: (1) the CS freezing resulted in better cryosurvival of bull sperm than UR freezing; (2) the RES supplementation to CS freezing medium improved sperm motility, membrane integrity, and fertility; and (3) despite low cryosurvival sperm and fertility, the RES addition to ultra-rapid freezing medium reduced oxidative stress.