Browsing by Author "Manzano Santana, Patricia Isabel"

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Acute oral toxicity of a novel functional drink based on Ilex guayusa, Vernonanthura patens, and cocoa husk
(2021) Iñiga Alvarado, Elsy Catalina; Sarmiento Tomalá, Glenda Marcela; Orellana Manzano, Andrea Katherine; Paladines Santacruz, Geovanna Lissette; Pilozo Muentes, Glenda Margarita; Berghe, Wim Vanden; Manzano Santana, Patricia Isabel; Villacres Cevallos, María del Carmen; Orellana Manzano, Silvia Gabriela; DI GRUMO, DAVIDE; Quijano Aviles, Maria Fernanda; Ortiz Ulloa, Silvia Johana; ; Zaruma Torres, Fausto Leonardo
A novel functional drink with nutraceutical properties was formulated from the aqueous extracts of Ilex guayusa, and Vernonanthura patens leaves, and cocoa husks. This juice contains various bioactive compounds, such as phenolic compounds and methylxanthines, with antioxidant and stimulant properties of pharmacological interest. However, it is known whether herbal extracts' interaction may have adverse toxic effects on human health. To evaluate this functional drink's innocuity, we estimated the acute oral toxicity (AOT) in experimental mice. This paper presents the AOT evaluation of two formulations of a functional drink (pre-formulation and microencapsulation) at a single dose of 2000 mg/kg of body weight (b.w.). No signs of adverse toxicity and mortality were observed after a single oral dose of 2000 mg/kg b.w. Likewise, no significant body and organ weight changes, food and water consumption behavior, and no histopathological changes were observed in the main organs evaluated. In conclusion, this functional drink can be categorized as low toxicity " according to the Globally Harmonized Classification System (GHS), making it a potential beverage with high nutritional and pharmacological value.
Diseño de un formulado microencapsulado con potencial propiedad nutracéutica, a partir de un extracto optimizado de Vernonanthura patens, Ilex guayusa y residuos de Theobroma cacao con distintos materiales de pared
(Universidad de Cuenca, 2023-05-12) Rodríguez Conterón, Oscar Paul; Manzano Santana, Patricia Isabel
Chlorogenic acids have favorable properties for human health and can be found in high amounts in the plant extract of Vernonanthura patens, Ilex guayusa and residues of Theobroma cacao; however, due to its vulnerability to variable environmental conditions, a protection mechanism is required that allows the effective delivery of its functionality. The objective of this work was to design a microencapsulated formulation by spray drying an extract of V. patens, I. guayusa and T. cacao residues with different wall materials, evaluate its efficiency and the bioaccessibility of specific phenolic compounds during gastrointestinal digestion (GID). The concentrated plant extract at 20 % and 40 % load was microencapsulated with maltodextrin (MD), mixtures of maltodextrin with gum arabic (MD-GA) and maltodextrin with pectin (MD-P). The results indicated the formulation that presented the most outstanding characteristics was MD-GA 1.5:1(p/p) loaded at 20%, which appeared as microparticles with moderate fluidity and intermediate cohesiveness and reached 61.8 ± 4.5% yield, 91.1 ± 8.3 % efficiency, with TPC of 13.5 ± 5.2 mg EAG/g, TFC of 25.8 ± 0.1 mgEQ/g and for DPPH 50.4 ± 1.4 µmolET/g. SEM, DSC and FTIR showed microcapsules with irregular morphology with a rough and seamless surface, which were more thermally stable and formed inclusion complexes. Furthermore, during simulated GID the greatest bioaccessibility occurred in the gastric mucosa, showing the feasibility to prepare a microencapsulated nutraceutical product on a larger scale.
Mortiño (Vaccinium floribundum Kunth): an underutilized superplant from the Andes
(2022) Llivisaca Contreras, Susana Alexandra; León Tamariz, Fabián; Manzano Santana, Patricia Isabel; Ruales Najera, Jeny Cumanda; Naranjo Moran, Jaime Alberto; Serrano Mena, Ana Lissette; Chica Martínez, Eduardo José; Cevallos Cevallos, Juan Manuel
Mortiño is a member of the Ericaceae family native to the Andes that has been used bylocal communities for centuries. This species has shown potential in the areas of medicine, agronomy,and green technology. We used a multidisciplinary approach to review aspects related to the ecology,horticulture, composition and potential biotechnological applications of mortiño. As interest in thisspecies grows, care must be taken to identify opportunities that justify its sustainable use whileemphasizing the development of local communities. Mapping the wide variety of potential uses andthe current state of conservation and utilization of this berry will help researchers to better targetmortiño’s potential