Browsing by Author "Corte Suqui, Jonathan Fabricio"

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    Evaluación del reconocimiento del antígeno IalB de Bartonella hanselae por anticuerpos de caninos (Canis lupus familiaris)
    (Universidad de Cuenca, 2025-09-30) Celi Cruz, Jorge Luis; Corte Suqui, Jonathan Fabricio; Vallecillo Maza, Antonio Javier
    Bartonella henselae (B. henselae), a Gram-negative bacterium responsible for bartonellosis, is a chronic disease that affects both animals and humans. In this context, cats (Felis catus domesticus) are the main carriers, and the disease is transmitted through their fleas (Ctenocephalides felis). In dogs, infection with this bacterium often does not induce the appearance of obvious clinical signs, which complicates its diagnosis at points of care. In addition, there are few diagnostic tools available to identify chronically infected animals. A possible indicator for detecting this infection in dogs (Canis lupus familiaris) is the IalB antigen, a protein found in the outer membrane of B. henselae. Therefore, the objective of this study was to evaluate the possible recognition of IalB by antibodies in dogs potentially exposed to B. henselae in the city of Cuenca, Ecuador. To achieve this objective, an indirect enzyme-linked immunosorbent assay (ELISA) was performed, using the recombinant IalB protein as the antigen together with a modified version of the same by adding a polystyrene-binding peptide. This assay was carried out on two types of plates. In the indirect ELISA assay, 92 canine sera were evaluated, in which the presence of fleas had been reported in a previous three-month period. MultiSorp and MaxiSorp plates were used for the analysis, and statistical tests were applied to compare reactivity. The Friedman test showed that, in the MultiSorp plates, there were no significant differences between the two antigens, indicating equivalent reactivity. However, in the MaxiSorp plates, lower antibody binding was observed. In addition, a high correlation between IalB and IalB-PoBP was evident in MultiSorp (ρ = 0.87; p < 0.0001), while a moderate correlation was observed in MaxiSorp (ρ = 0.65), suggesting that fusion to the peptide does not substantially alter recognition. Finally, no correlation was found between the presence of fleas and antibody detection (ρ ≈ 0.0; p > 0.7), which could be attributed to limitations in risk assessment based on medical history and physical examination.