Browsing by Author "Campoverde Cisneros, Manuel Alfredo"

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A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.
(2019) Rojas, Glenda C.
Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was …
Antígenos HLA de clase II en pacientes con artritis reumatoide ecuatorianos y asociación con el factor reumatoide, anticuerpos anti-citrulinados y nódulos reumatoides
(2014) Vintimilla Ugalde, Jaime Fernando
Objective To determine Class II antigens in 52 patients with rheumatoid arthritis (RA) and 52 controls and establish the association of Class II antigens DRB1* with rheumatoid anti- cyclic citrullinated peptide (anti-CCP) rheumatoid factor (RF), and antibodies rheumatoid nodules. Methods and Materials Case and control study in patients from the Hospital Vicente Corral Moscoso and José Carrasco Arteaga of Cuenca-Ecuador; 52 patients with rheumatoid arthritis who satisfy the criteria of ACR-American College of Rheumatology and 52 healthy patients. The different types of HLA from each 52 patients with rheumatoid arthritis and controls were identified through PCR - Polymerase Chain Reaction. Association of HLA-DR1* with rheumatoid arthritis and rheumatoid factor, anti-citrullinated and rheumatoid nodules. The statistical analysis was done by the OR (IC 95%) and 2 of Pearson with Fisher correction and the information was processed in SPSS v15. Results The most frequency alleles were HLA-DRB1*09 (17,3%) OR 3,42 (IC95% 1,21 – 10,14) and HLADRB1* 04 (32,7%) OR 1,81 (IC95% 0,93 – 3,55). The rheumatoid factor and anti-cyclic citrullinated peptide antibodies were presented in 82,7% of patients with rheumatoid arthritis and rheumatoid nodules was detected in the 19.2% of patients with rheumatoid arthritis . An association between rheumatoid nodules with high values of rheumatoid factor and anti-ccp and these in turn with HLA-DRB1*14 y HLA-DRB1*04 alleles were found. The majority of patients with HLA-DRB1*09 alleles with heterozygote predomination had positive anti-ccp and rheumatoid factor. Conclusion The presence of HLA-DRB1*04 and HLA-DRB1*09 alleles is related with the susceptibility of present rheumatoid arthritis in Ecuadorian patients, and it is related with higher values of rheumatoid factor and anti-cyclic citrullinated peptide.
Caracterización de los genotipos frecuentes del virus del papiloma humano en mujeres atendidas en los hospitales Monte Sinai y del Seguro Social Cuenca- Ecuador. 2008- 2014
(2017) Arcentales Cayamcela, Mauro Javier
OBJECTIVE: To determine the prevalence of hu- man papilloma virus genotypes in cervical-uterine samples and their relation to risk factors in women with active sex life in Cuenca. METHODOLOGY: It is a cross-sectional observatio- nal study with a sample of 500 women from Cuen- ca who were attended in the Monte Sinai Hospi- tal and the Ecuadorian Institute of Social Security (IESS). The exams were carried out in the BIONCO- GEN Molecular Biology Laboratory which is loca- ted in Cuenca. The Real Time PCR technique plus the Pap examination were used. RESULTS: The prevalence of HPV was 78.4 % and the most frequent genotype was the HPV 16 with 26.2 %. On the other hand, the CIN I with 27% is the most frequent gynecological pathology. CONCLUSION: The genotypes 16/18 represent the types of HPV that are directly related to the de- velopment of cervical cancer because when it is correlated with cytology (Pap) it is demonstrated that the cell undergoes morphological changes (koilocytosis).
Caracterización de los genotipos frecuentes del virus del Papiloma Humano en mujeres atendidas en los hospitales Monte Sinai y del Seguro Social Cuenca- Ecuador. 2008- 2014
(Universidad de Cuenca. Facultad de Ciencias Médicas, 2017-04) Campoverde Cisneros, Manuel Alfredo; Arcentales Cayamcela, Mauro; Caguana Mayancela, John Patricio
OBJECTIVE: To determine the prevalence of human papilloma virus genotypes in cervical-uterine samples and their relation to risk factors in women with active sex life in Cuenca. METHODOLOGY: It is a cross-sectional observational study with a sample of 500 women from Cuenca who were attended in the Monte Sinai Hospital and the Ecuadorian Institute of Social Security (IESS). The exams were carried out in the BIONCOGEN Molecular Biology Laboratory which is located in Cuenca. The Real Time PCR technique plus the Pap examination were used. RESULTS: The prevalence of HPV was 78.4 % and the most frequent genotype was the HPV 16 with 26.2 %. On the other hand, the CIN I with 27% is the most frequent gynecological pathology. CONCLUSION: The genotypes 16/18 represent the types of HPV that are directly related to the development of cervical cancer because when it is correlated with cytology (Pap) it is demonstrated that the cell undergoes morphological changes (koilocytosis). Keywords: Genotype, Human papillomavirus 16, Human papillomavirus 18, Clinical Laboratory Techniques, PCR; Risk factors; Monte Sinai Hospital, Hospital of the Ecuadorian Institute of Social Security, Cuenca-Ecuador.
Conocimiento y actitudes sobre el virus de inmunodeficiencia humana en adultos jóvenes en la consulta externa, Centro de Salud Pumapungo, Cuenca. 2024-2025
(Universidad de Cuenca, 2025-04-02) Velasco Medrano, Johnny Azael; Valverde Ordoñez, Erika Daniela; Campoverde Cisneros, Manuel Alfredo
The Human Immunodeficiency Virus (HIV) is considered a global epidemic due to the increase in cases of transmission among young adults due to risky sexual behaviors generated by the lack of knowledge of means of transmission and preventive measures. The general objective is to identify the knowledge and attitudes of young adults about the Human Immunodeficiency Virus in the Outpatient Clinic, Pumapungo Health Center, Cuenca, Ecuador, 2024-2025. 2024- 2025; it was analyzed through a cross-sectional descriptive analytical observational quantitative cross-sectional study, with a sample of 296 people and two questionnaires were} applied on the level of knowledge of prevention and attitudes about HIV/AIDS collected and coded by KoboToolbox. SPSS (version 30.0) was used for data tabulation and analysis. According to the results of the research, knowledge was 36.5% regular, 30.4% good, 28% deficient and only 5.1% excellent; while in attitudes 99.7% are positive and only 0.3% are negative towards HIV-positive people. In conclusion, this information describes different levels of understanding of the virus, both in their sexual education and in the treatment of people living with HIV, influenced by social and cultural factors, so it is suggested to implement new strategies with a specific approach to maintain the interest of this group and at the same time give them feedback.
Conocimientos, actitudes y prácticas sobre papiloma virus humano en estudiantes de la Escuela de Tecnología Médica-Universidad de Cuenca, 2016
(Universidad de Cuenca, 2017-11-11) Sinchi Tasambay, Hilda Maruja; Yupa Alvarez, Valeria Jaqueline; Macero Méndez, Reina María; Campoverde Cisneros, Manuel Alfredo
Background: It is very important to find out the knowledge they have the / os students with respect to Human Papillomavirus because adolescents to have an active sex life at an early age are more likely to contract the infection.1 Objective: To identify knowledge, attitudes and practices on human papilloma virus in students from the School of Medical Technology at the University of Cuenca 2016. Methodology: descriptive study, which allowed knowing the knowledge, attitudes and practices on human papilloma virus. The universe was 660 students of the School of Medical Technology; the sample was made up 243 students which were obtained by applying a standard deviation of the population of 0.5, a confidence level of 95% and an acceptable limit of sampling error, 5% (0.05). Prior to the development of research students gave written informed consent, surveys was applied with information regarding the study variables: age, sex, marital status, race, age of first sexual intercourse, sexual partners, knowledge, attitudes and practices on HPV. Scorekeeping for the relevant forms were used and processed for tabulation in Microsoft Word, Microsoft Excel and SPSS. Results: 65.8% mentioned receiving information about HPV, recognize that affects both men and women 69.1%. 28% do not know that complications can bring HPV infection; 37.9% indicated having active sex life
Detección de la mutación JAK2 V617F en neoplasias mieloproliferativas en población ecuatoriana por reacción en cadena de la polimerasa alelo específica
(2017) Campoverde Cisneros, Manuel Alfredo; Oliveros Alvear, Jorge William; Reyes Peña, Ines Maria; Maldonado Guerrero, Bella Irma; Becerra Navarrete, Edgar Antonio; Ullauri Zambrano, Veronica Alexandra; Villa Cardenas, Esteban Homero; Espinoza Calle, Glenda Iralda; Ladines Castro, Washington Javier; Chacon Velez, Jennifer Yadira; Arcentales Cayamcela, Mauro Javier
In 2005, five independent research groups reported the association of V617F mutation in the gene encoding the janus kinase 2 (JAK2) Myeloproliferative Neoplasms Small (NMP), including polycythemia vera (PV), Essential thrombocytosis (ET) and primary myelofibrosis (MFP). This study aimed to detect the JAK2 V617F mutation in Ecuadorian patients through chain reaction polymerase allele specific (AS-PCR). The research design was descriptive cross-sectional and included a total of 40 patients, 20 with NMP and 20 controls attending the various services Haematology Health Institutions participating. Genetic material was extracted and then amplified by AS-PCR, while the clinical hematology of NMP was performed by using specialized clinical, morphological and hematological tests such as blood counts and flow cytometry examination hematologists. The frequency of the JAK2 V617F mutation found was 50% with a rate of 95% confidence. Patients with a greater presence of the mutation (35%) were those with PV, followed by patients with TE (15%), the average age being 45. These data confirm that the JAK2 V617F mutation is prevalent in NMP and has become the molecular marker of both the PV, ET and MFP. Likewise, this study has shown that the AS-PCR method is a quick, easy and cost-effective diagnosis.
Diagnóstico de enfermedades de transmisión sexual mediante PCR tiempo real en mujeres indígenas del Ecuador 2016
(Universidad de Cuenca, 2017-11-11) Caguana Mayancela, John Patricio; Carreño Calle, Christian Santiago; Campoverde Cisneros, Manuel Alfredo
Background Screening test for the detection of sexually transmitted diseases, some of them producing cancer such as human papilloma virus, besides being factors of infertility and infertility, depends increasingly on molecular techniques. An important challenge for developing countries like Ecuador has been the lack of availability and costs of tests based on the detection of the DNA of the microorganism. In this study, microorganisms of sexual transmission were diagnosed by qPCR in women of indigenous nationality Ecuador. Methodology Inside the laboratory of molecular biology of the Cuenca University, a descriptive study was carried out to diagnose sexually transmitted microorganisms in cervical samples using molecular techniques of HPV-28 and STI-7 Anyplex qPCRs. The samples came from woman indigenous from the provinces of Loja, Morona Santiago and Cañar. Results 396 samples were analyzed, 120 samples were extracted in Saraguro, 131 in Quilloac community, 145 in Seville Don Bosco community. 135 samples showed high risk and low oncogenic HPV risk genotypes, also 362 samples with other sexually transmitted microorganisms. Conclusions Implementing molecular techniques such as qPCR, for the diagnosis of microorganisms of sexual transmission are fast, simple with a high sensitivity and specificity
Estado nutricional y su influencia en el rendimiento académico en niños de 6 a 11 años de la Unidad Educativa Octavio Diaz León Parroquia “El Valle”, 2024-2025
(Universidad de Cuenca, 2025-07-29) Chuqui Guallpa, Deysi Estefania; Suquilanda Domínguez, Yamileth Estefania; Campoverde Cisneros, Manuel Alfredo
Introduction: The relationship between nutritional status and academic performance has garnered increasing attention in health and education research due to its impact on children's cognitive and psychosocial development during early childhood. This relationship is closely linked to the family and community environment in which children grow. Objective: This study aimed to examine the connection between nutritional status and academic performance in children aged 6 to 11 years at the Octavio Díaz León Educational Unit in the "El Valle" Parish. Methodology: An observational, analytical, cross-sectional cohort study with a quantitative approach was conducted. The study sample consisted of 99 children, selected through a sampling formula from a universe of 132 students. Demographic data such as age, gender, and educational level were collected. Nutritional status was assessed using anthropometric measurements, including weight, height, and Body Mass Index (BMI), which were interpreted based on percentile curves. Academic performance was evaluated using grades from the school year, in accordance with the Ministry of Education's grading system. Data analysis was performed using SPSS software, with frequency percentages and the Chi-square test to assess the relationship between nutritional status and academic performance. Results: The highest percentage of children (26.3%) were aged 9 years. According to percentile curves, 67.7% of children were classified as having normal weight, while overweight and obesity rates were 10.5% and 18.8%, respectively. A significant association was found between nutritional status and academic performance (p < .001), particularly among children with normal weight. Conclusion: Children with normal weight demonstrated better academic performance, with 33% of these children showing improved learning outcomes. These findings underscore the importance of implementing nutritional interventions to enhance school performance and overall well-being.
Estrés laboral en bomberos de las estaciones de Cuenca - Ecuador, 2024 - 2025
(Universidad de Cuenca, 2025-05-05) Romero Méndez, Katherine Vanessa; Reinoso Peláez, Larissa Lisbeth; Campoverde Cisneros, Manuel Alfredo
The World Health Organization (WHO) states that work stress is a psychological state that generates emotional, cognitive, physiological, and behavioral reactions to high stress situations at work. For example: firefighters, who intervene in emergency situations; exposing themselves daily to traumatic and stressful situations; resulting in psychological changes that can damage their mental health. Objective: To determine the level of work stress in firefighters of Cuenca - Ecuador, 2024 - 2025. Methodology: A qualitative, descriptive, analytical, and cross-sectional study was conducted. The population of our study was 105 firefighters working in the different stations of Cuenca, data provided by the Benemérito Cuerpo de Bomberos (Meritorious Fire Department). The validated instrument we used was the ILO-WHO Work Stress Scale, which was evaluated by means of a survey designed in Kobo ToolBox to assess the general perception of work stress of the workers at a given time. The data collected was processed and analyzed using the Statistical Package for the Social Sciences (SPSS) version 30.0.0. Results: We evidenced the level of occupational stress that firefighters have, identifying the association it has with sociodemographic variables; consequently, these findings lay the empirical foundations necessary for the development and implementation of strategies to orient the firefighter's work.
Experiencias asociadas al consumo de sustancias psicoactivas en pacientes internos del Centro “Paz y Vida”, Milagro 2024-2025
(Universidad de Cuenca, 2025-09-02) Herrera Sánchez, Angie Lissette; Alcivar Santana, Emely Jaidy; Campoverde Cisneros, Manuel Alfredo
The use of psychoactive substances has evolved from its cultural and ritualistic origins to become a significant global public health issue, affecting individuals of all ages and social backgrounds. This study aimed to describe the experiences associated with substance use among patients admitted to the "Paz y Vida" rehabilitation center in Milagro during 2024-2025. Using a qualitative phenomenological approach, in-depth interviews were conducted with 15 male patients. Data collection involved recording, transcription, and analysis using Alasia 24.2.0 software, employing categorization and coding techniques. Findings revealed key aspects of the participants' experiences, including the age of substance use initiation, influential factors such as family and social environments, and the most commonly consumed substances, notably alcohol. The study also explored consumption methods, consequences of substance use, and associated risks, including homelessness and life-threatening situations. Furthermore, the research highlighted the patients' progress during their rehabilitation process and the positive changes observed in their lives. This study underscores the need for a comprehensive understanding of substance abuse, considering the lived experiences of affected individuals, and emphasizes the critical role of rehabilitation centers in supporting recovery and improving life outcomes.
Human Papillomavirus Prevalence and Associated Factors in Indigenous Women in Ecuador: a Cross-Sectional Analytical Study
(2023) Ortiz Segarra, José Ignacio; Vega Crespo, Bernardo José; Salazar Torres, Zoila Katherine; Delgado López, Dayanara Alejandra; Ortiz Mejía, José Stalin; Campoverde Cisneros, Manuel Alfredo
Abstract: Cervical cancer (CC) is the second leading cause of death from malignancy in women in Ecuador. Human papillomavirus (HPV) is the main causative agent of CC. Although several studies have been conducted on HPV detection in Ecuador, there are limited data on indigenous women. This cross-sectional study aimed to analyze the prevalence of HPV and associated factors in women from the indigenous communities of Quilloac, Saraguro and Sevilla Don Bosco. The study included 396 sexually active women belonging to the aforementioned ethnicities. A validated questionnaire was used to collect socio-demographic data, and real-time Polymerase Chain Reaction (PCR) tests were used to detect HPV and other sexually transmitted infections (STIs). These communities are located in the southern region of Ecuador and face geographical and cultural barriers to accessing health services. The results showed that 28.35% of women tested positive for both types of HPV, 23.48% for high-risk (HR) HPV, and 10.35% for low-risk (LR) HPV. Statistically significant associations were found between HR HPV and having more than three sexual partners (OR 1.99, CI 1.03–3.85) and Chlamydia trachomatis infection (OR 2.54, CI 1.08–5.99). This study suggests that HPV infection and other sexually transmitted pathogens are common among indigenous women, highlighting the need for control measures and timely diagnosis in this population
Microorganismos frecuentes y resistencia antimicrobiana en muestras biológicas en el área de neonatología del Hospital Vicente Corral Moscoso 2015
(Universidad de Cuenca, 2017-11-11) Méndez Padilla, Fabián Patricio; Niola León, Flavio Hernán; Macero Méndez, Reina María; Campoverde Cisneros, Manuel Alfredo
Background: The immunological immaturity of newborns involves a greater susceptibility to infections by various microorganisms, including those traditionally innocuous. Intra-hospital infections are an important cause of morbidity and mortality in neonates. Objective: Determine the frequent and antimicrobial resistance of microorganisms in biological samples of neonates in “Vicente Corral Moscoso” Hospital, during January-December 2015. Methodology: A descriptive, retrospective and observational study was conducted, based on the data from microbiology area in “Vicente Corral Moscoso” Hospital. Bacterial isolates and antimicrobial resistance in biological samples of neonatology during 2015 were examined. The development of the database and statistical analysis were done on the programs SPSS v22 and Microsoft Excel. The variables were described using frequency tables and percentages. Results: 343 medical records were analyzed, and a total of 343 microorganisms were found in 11 types of biological samples which were classified into 27 bacterial species. Klebsiella pneumoniae was the most frequent bacteria with 26.5% and the most resistant with 45.2%. A total of 2885 resistances to 37 different types of antibiotics were counted, being Ampicillin the antibiotic to which the bacteria showed greater resistance to, with 9.5%
Percepciones sobre la salud sexual y reproductiva de las mujeres indígenas Kichwas y Shuaras. Ecuador 2016
(2016) Ortiz Segarra, José Ignacio; Freire Argudo, Marco Vinicio; Palacios Espinoza, Elvira del Carmen; Vega Crespo, Bernardo José; Jiménez Brito, Doriz Angélica; Campoverde Cisneros, Manuel Alfredo; Muñoz Tigrero, Lorena Cristina eulalia; Alvarado Verdugo, Libia Graciela
Cervical cancer is a health problem unresolved in de-veloping countries; this disease is the leading cause of death from gynecologic cancer in Ecuador. Coverage of preventive screening is influenced by cultural con-ceptions of the population, which are based on the per-ceptions of each ethnic group on the etiologic process health-disease complex.OBJECTIVE: To interpret the perceptions of women of in-digenous peoples and nationalities Shuar of Sevilla Don Bosco and Kichwas of Quilloac and Saraguro, about sexual and reproductive health, sexually transmitted in-fections, cervical cancer and associated conditions. METHODOLOGY: A qualitative, phenomenological study was conducted through 17 focus groups and 13 stories of life study. A total of 102 people interviewed. RESULTS: The stories of women understood as collective constructions of their respective ethnic groups in the context of a Western, patriarchal and maleness hege-monic culture, mainly refer to destructive processes of sexual and reproductive health, manifested as infideli-ty and male promiscuity and as the lack of protection with condoms, which affects the prevalence of sexually transmitted infections and Cervical Cancer. CONCLUSIONS: Women have awareness of a broad concept of health, including notions of sexual and re-productive health; however, relate obstacles in their full exercise, related to inequalities of class, gender, and generational etnia1 expressed as destructive processes of their health
Prevalencia de genotípos del papiloma virus en mujeres de Cuenca
(Universidad de Cuenca. Facultad de Ciencias Médicas, 2014-04) Cárdenas Herrera, Oswaldo José; Cabrera Vicuña, José Antonio; Campoverde Cisneros, Manuel Alfredo
OBJECTIVES. To determine the prevalence of papilloma virus genotypes of high and low oncogenic grade, to relate to age groups, with genotypes that avoid both existing vaccines and the risk factors for uterine cervical cancer. METHODS. Epidemiological, observational, cross-sectional study. The random sample was 500 women from the urban area of Cuenca city. Two samples of the cervix were taken. Polimeraza chain reaction method and cytopathology Papanicolaou study were used. RESULTS. The prevalence of papilloma virus genotypes of high oncogenic grade was 35,9% and 14,3% low oncogenic grade. The prevalence of cytologic abnormalities, Papanicolaou, was 16%. This prevalence was more frequent in the group of 30-39 years, and 40-50 years. By inference, the coverage of the Cervarix vaccine would be 8% and 13% for Gardasil. Risk factors were related to the number and frequency of intercourse and number of sexual partners. CONCLUSION. The prevalence of genotypes was high and the vaccine coverage was low.
Prevalencia de genotipos del papiloma virus humano en mujeres de la provincia del Azuay, Ecuador
(Universidad de Cuenca, 2015-06) Cabrera V., José A.; Cárdenas Herrera, Oswaldo; Campoverde Cisneros, Manuel Alfredo; Ortiz Segarra, José Ignacio; Universidad de Cuenca; Dirección de Investigación de la Universidad de Cuenca; DIUC
The main objectives of the research were to detect as a function of age the prevalence of high- and low-risk oncogenic Human Papillomaviruses (HPV) in cervical samples of woman in the fourteen districts of the province of Azuay. The project encompassed the cytopathological diagnosis of intraepithelial cervical lesions and the risk factors of the genotypes found were related with existing vaccines which are used as a preventive measure of cervical cancer. Cervical smear samples from a random population of 500 women were screened with the Papanicolaou (Pap) test using real-time polymerase chain reaction (PCR). The study revealed a prevalence of HPV of 25.6%, respectively 4.8% low-risk oncogenic genotypes and 20.8% high-risk oncogenic genotypes, and only in the 20-29 age group a significant higher prevalence of the high-risk genotypes 31 and 66 (p<0.05) was observed. Atypical squamous cells of undetermined significance (ASCUS) represent 7% and the low-grade squamous intraepithelilial lesion (LSIL) 1.8%. Furthermore no high-grade squamous intraepithelilial lesions were identified. Of the surveyed population 2.8% of the women possess viral genotypes which are treatable by the vaccines distributed by the Ministry of Public Health (MSP).
Prevalencia de genotipos del papiloma virus humano en mujeres de la provincia del Azuay, Ecuador
(2015) Campoverde Cisneros, Manuel Alfredo; Ortiz Segarra, José Ignacio; Cabrera Vicuña, José Antonio; Cárdenas Herrera, Oswaldo José
The main objectives of the research were to detect as a function of age the prevalence of high-and low-risk oncogenic Human Papillomaviruses (HPV) in cervical samples of woman in the fourteen districts of the province of Azuay. The project encompassed the cytopathological diagnosis of intraepithelial cervical lesions and the risk factors of the genotypes found were related with existing vaccines which are used as a preventive measure of cervical cancer. Cervical smear samples from a random population of 500 women were screened with the Papanicolaou (Pap) test using real-time polymerase chain reaction (PCR). The study revealed a prevalence of HPV of 25.6%, respectively 4.8% low-risk oncogenic genotypes and 20.8% high-risk oncogenic genotypes, and only in the 20-29 age group a significant higher prevalence of the high-risk genotypes 31 and 66 (p<0.05) was observed. Atypical squamous cells of undetermined significance (ASCUS) represent 7% and the low-grade squamous intraepithelilial lesion (LSIL) 1.8%. Furthermore no high-grade squamous intraepithelilial lesionswere identified. Of the surveyed population 2.8% of the women possess viral genotypes which are treatable by the vaccines distributed by the Ministry of Public Health (MSP)
Programa educativo para la prevención del cáncer de cuello uterino
(Universidad de Cuenca, 2017) Campoverde Cisneros, Manuel Alfredo; Jiménez Brito, Doriz Angélica; Vega Crespo, Bernardo José; Salazar Torres, Zoila Katherine; Freire Argudo, Marco Vinicio; Ortiz Segarra, José Ignacio; Palacios Espinoza, Elvira del Carmen; Murillo Bacilio, Magdali del Rocío; Viñansaca Atancuri, Lourdes Catalina
Reciprocal contribution of clinical studies and the HP10 antigen ELISA for the diagnosis of extraparenchymal neurocysticercosis.
(2018) Parkhouse, Michael
Abstract To evaluate diagnosis of active neurocysticercosis, paired cerebral spinal fluid (CSF) and serum samples from 24 neurocysticercosis (NCC) patients and 17 control neurological patients were assayed in the HP10 Taenia antigen (Ag) ELISA. The CSF samples were also tested with an HP10 Lateral Flow Assay (LFA). The HP10 Ag was detected by ELISA in the CSF of 5/5 patients with Definitive extraparenchymal NCC, and in 4/5 of the corresponding sera. In the Definitive parenchymal group, on the other hand, the HP10 Ag was absent in 2/3 CSF (with a very low value in the one positive sample) and all the corresponding serum samples. Samples of CSF from 4/7 patients in the Probable parenchymal group, were also significantly HP10 Ag positive, suggesting the presence of extraparenchymal cysts not identified by the imaging studies. With the possible exception of one patient, the corresponding serum samples of the Probable parenchymal NCC group, were all HP10 Ag negative. Samples of CSF from 9 NCC patients diagnosed with Mixed parenchymal and extraparenchymal NCC were all significantly HP10 Ag positive, confirming the presence of extraparenchymal cysts, with only 7/9 of the corresponding serum samples being HP10 positive. Thus detection of the HP10 Ag indicates extraparenchymal and not parenchymal cyst localization and is more sensitive with CSF than serum. Three neurological patients clinically diagnosed as subarachnoid cyst, hydrocephalus and tuberculoma, respectively, were clearly positive for HP10 Ag. Of these, two were confirmed as NCC by subsequent imaging; the third died prior to further examination. Thus, a total of 8 patients had their clinical diagnosis questioned. Finally, there was good agreement between the HP10 Ag ELISA and LFA with CSF samples giving an optical density ≥0.4 in the ELISA assay. In conclusion, the HP10 Ag assay should provide a valuable and reciprocal tool in the clinical diagnosis and follow up of extraparenchymal NCC.
SECUELAS POSCOVID-19. REVISION SISTEMATICA
(Centro de Estudios Sociales de América Latina (CES—AL), 2022) Morocho Morocho, Sergio Ronaldo
This study has been developed as a bibliographic and literary review to analyze the post- Covid-19 sequelae in Ecuador and Latin America, as the definition of the referential research environment, which contributes to the wealth of health, clinical, medical and public health knowledge, considering the importance of the current pandemic that the world is going through. The research objective was to analyze the consequences of Covid- 19, to form a criterion based on facts based on referential studies that contribute to the knowledge of the current scenario. The methodology is qualitative, due to the fact that 31 scientific articles and research appropriate to the topic of the pandemic were reviewed, especially on analyzes in institutionalized patients due to SARS-CoV-2, coronavirus 2, which estimated a situation of serious context and I criticize mainly, to observe the evolution of health and the consequences after the disease. The results showed that, although there is a clearer picture since the pandemic began in 2020, a specific clinical criterion for Covid-19 cannot yet be constructed, because the current studies are still in the testing phase, from research and development. results in all age ranges and sixths around the world. Consequently, it is intended to contribute to the development of medical and health knowledge, maintaining the perspective of constant evolution of the disease in the population.