Browsing by Author "Bosmediano Begay, Evelyn Melissa"

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    Efecto de la adición de tres factores de crecimiento (EGF, LIF, ITS) y su combinación sobre la maduración in vitro de ovocitos de cobaya
    (Universidad de Cuenca, 2025-04-16) Bosmediano Begay, Evelyn Melissa; Victoria Grefa, Erick Jossue; Perea Ganchou, Fernando Pedro
    This study evaluated the effect of adding epidermal growth factor (EGF), leukemia inhibitory factor (FIL), insulin-transferrin-selenium (ITS), and their combination on the in vitro maturation (IVM) of guinea pig oocytes. Ovaries were collected from a local slaughterhouse and transported to the laboratory at 34–37 °C. Cumulus-oocyte complexes (COCs) were recovered by slicing and selected based on morphological quality; only suitable COCs were used for IVM. Selected COCs were randomly assigned to five treatments: T0 (control): base medium (BM) composed of TCM-199 supplemented with sodium pyruvate, glutamine, gentamicin, and bovine serum albumin; T1: BM + 50 ng/ml EGF; T2: BM + 100 ng/ml FIL; T3: BM + 5 µg/ml ITS; and T4: BM + EGF, FIL, and ITS combined at the same concentrations. All groups were incubated for 24 hours. Cumulus expansion was evaluated by measuring cluster thickness before and after IVM, and nuclear maturation status was determined using Hoechst staining. Data were analyzed by ANOVA and the LSMEAN test. The addition of EGF, FIL, ITS, or their combination did not significantly affect the rate of nuclear maturation, which ranged from 16.3% to 24.8%, nor did it influence the proportion of immature or degenerated oocytes. However, cumulus thickness was significantly affected by treatment (P < 0.0001). In conclusion, while the supplementation of growth factors did not improve nuclear maturation in guinea pig oocytes, it did influence cumulus cell expansion during in vitro maturation.