Facultad Ciencias Agropecuarias Tesis Maestrías

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Evaluación de la aplicación de un ensayo LAMP para el diagnóstico de Parvovirus canino tipo 2 (CPV-2) en heces
(Universidad de Cuenca, 2021-09-29) Alvarado Dávila, Paola Gabriela; Palacios Ordóñez, Teófilo Estuardo
Canine parvovirus type 2 or (CPV-2) is a contagious infectious disease, the main cause of bloody diarrhea in puppies from 2 to 6 months of age, it is also known as canine viral enteritis and is produced by a DNA virus of In the family Parvoviridae, of the genus Parvovirus, the most common clinical signs are: anorexia, lethargy, fever, vomiting, diarrhea and dehydration. Its diagnosis is made by means of an immunochromatography test for the detection of CPV-2 antigen, said test can be negative if the analysis is performed very early in the clinical course of the disease, another test used is LAMP, which is It is a unique method of gene amplification. The objective of this research was to identify the presence of CPV-2 genetic material by applying the LAMP diagnostic technique and the immunochromatography test on swabs of stool from dogs that presented clinical signs compatible with canine parvovirus. The results with the immunochromatography test showed that 8 of the 62 samples were positive for CPV-2 DNA, while in the LAMP test, 32 were positive for CPV-2 DNA out of a total of 62 samples analyzed; the sensitivity of the LAMP assay was 62.5%, while the specificity was 50%.