Prevalencia de cepas de escherichia coli productoras de betalactamasas de espectro extendido (blee) en muestras de orina de pacientes ambulatorios de los Centros de Salud 1, 2 y 3 de la ciudad de Cuenca

Abstract

Analyzed a total of 274 urine samples from outpatients presenting to Health Centres 1, 2 and 3 of the city of Cuenca during the period between May and June 2013, with the purpose to get at least 100 samples positive urine with Escherichia coli. Were retrieved 103 strains of Escherichia coli and the study was continued with the identification of the production of Extended Spectrum Beta Lactamases (ESBL), using the technique described in the manual updated CLSI January 2013. Were performed presumptive and confirmatory tests, applying the conventional method of agar diffusion plates in Mueller-Hinton agar, with a 0.5 McFarland inoculum and tested antimicrobial discs. For the presumptive test was used discs Aztreonam, Cefotaxime, Ceftazidime and Ceftriaxone, for the confirmatory test were used discs of Ceftazidime and Cefotaxime in combination with clavulanic acid. ESBL production was determined by the difference in the diameter of the halos as indicated in the technique. The results showed that of 103 strains of E. coli recovered seven (6.8%) ESBL producing strains, when considering that the population which was worked was of outpatient, is very important the implementation of identification methods as ESBL for correct support of antimicrobial therapy, preventing this way the spread of strains of E. coli ESBL producing