A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.

Parkhouse, Michael; Campoverde Cisneros, Manuel Alfredo; Harrison, Leslie; Carpio Rodas, Luis Arturo; Cortéz, María Milagros; Rojas, Glenda C.; Sastre, Patricia

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Título :	A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.
Autor:	Parkhouse, Michael Campoverde Cisneros, Manuel Alfredo Harrison, Leslie Carpio Rodas, Luis Arturo Cortéz, María Milagros Rojas, Glenda C. Sastre, Patricia
Correspondencia:	Parkhouse, Michael, parkhous@igc.gulbenkian.pt
Palabras clave :	Neurocysticercosis (NCC) Field diagnosis Bovine cysticercosis HP10 antigen ELISA HP10 antigen LFA
Área de conocimiento FRASCATI amplio:	3. Ciencias Médicas y de la Salud
Área de conocimiento FRASCATI detallado:	3.3.1 Ciencias y Servicios del Cuidado de la Salud
Área de conocimiento FRASCATI específico:	3.3 Ciencias de la Salud
Área de conocimiento UNESCO amplio:	09 - Salud y Bienestar
ÁArea de conocimiento UNESCO detallado:	0912 - Medicina
Área de conocimiento UNESCO específico:	091 - Salud
Fecha de publicación :	2019
Fecha de fin de embargo:	29-jul-2050
Volumen:	vol. 113
Fuente:	Transactions of the Royal Society of Tropical Medicine and Hygiene
metadata.dc.identifier.doi:	10.1093/trstmh/try116.
Tipo:	ARTÍCULO
Abstract:	Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was …
Resumen :	Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was …
URI :	http://dspace.ucuenca.edu.ec/handle/123456789/42383 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061121773&origin=inward
URI Fuente:	http://www.sciencedirect.com/science/journal/00359203
ISSN :	0035-9203
Aparece en las colecciones:	Artículos

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