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DC Field | Value | Language |
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dc.contributor.author | Parkhouse, Michael | |
dc.contributor.author | Campoverde Cisneros, Manuel Alfredo | |
dc.contributor.author | Harrison, Leslie | |
dc.contributor.author | Carpio Rodas, Luis Arturo | |
dc.contributor.author | Cortéz, María Milagros | |
dc.contributor.author | Rojas, Glenda C. | |
dc.contributor.author | Sastre, Patricia | |
dc.date.accessioned | 2023-07-12T17:25:46Z | - |
dc.date.available | 2023-07-12T17:25:46Z | - |
dc.date.issued | 2019 | |
dc.identifier.issn | 0035-9203 | |
dc.identifier.uri | http://dspace.ucuenca.edu.ec/handle/123456789/42383 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061121773&origin=inward | |
dc.description | Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was … | |
dc.description.abstract | Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was … | |
dc.language.iso | es_ES | |
dc.source | Transactions of the Royal Society of Tropical Medicine and Hygiene | |
dc.subject | Neurocysticercosis (NCC) | |
dc.subject | Field diagnosis | |
dc.subject | Bovine cysticercosis | |
dc.subject | HP10 antigen ELISA | |
dc.subject | HP10 antigen LFA | |
dc.title | A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives. | |
dc.type | ARTÍCULO | |
dc.ucuenca.idautor | 0102517265 | |
dc.ucuenca.idautor | Srgp-1044-6 | |
dc.ucuenca.idautor | Srgp-1044-7 | |
dc.ucuenca.idautor | 0000-0002-6143-2160 | |
dc.ucuenca.idautor | 0100737956 | |
dc.ucuenca.idautor | Srgp-1044-5 | |
dc.ucuenca.idautor | 0000-0003-3732-5047 | |
dc.identifier.doi | 10.1093/trstmh/try116. | |
dc.ucuenca.embargoend | 2050-07-29 | |
dc.ucuenca.version | Versión publicada | |
dc.ucuenca.embargointerno | 2050-07-29 | |
dc.ucuenca.areaconocimientounescoamplio | 09 - Salud y Bienestar | |
dc.ucuenca.afiliacion | Parkhouse, M., Instituto de Ciencias Gulbenkian, Oeiras, Portugal | |
dc.ucuenca.afiliacion | Campoverde, M., Universidad de Cuenca, Facultad de Ciencias Médicas, Cuenca, Ecuador | |
dc.ucuenca.afiliacion | Sastre, P., INMUNOLOGÍA Y GENETICA APLICADA SA, Madrid, España | |
dc.ucuenca.afiliacion | Rojas, G., Universidad de Carabobo, Valencia, Venezuela | |
dc.ucuenca.afiliacion | Cortéz, M., Universidad de Carabobo, Valencia, Venezuela | |
dc.ucuenca.afiliacion | Harrison, L., University of Edinburgh, Edinburgh, Reino unido | |
dc.ucuenca.afiliacion | Carpio, L., Universidad de Cuenca, Facultad de Ciencias Médicas, Cuenca, Ecuador | |
dc.ucuenca.correspondencia | Parkhouse, Michael, parkhous@igc.gulbenkian.pt | |
dc.ucuenca.volumen | vol. 113 | |
dc.ucuenca.indicebibliografico | SCOPUS | |
dc.ucuenca.factorimpacto | 1.175 | |
dc.ucuenca.cuartil | Q1 | |
dc.ucuenca.numerocitaciones | 0 | |
dc.ucuenca.areaconocimientofrascatiamplio | 3. Ciencias Médicas y de la Salud | |
dc.ucuenca.areaconocimientofrascatiespecifico | 3.3 Ciencias de la Salud | |
dc.ucuenca.areaconocimientofrascatidetallado | 3.3.1 Ciencias y Servicios del Cuidado de la Salud | |
dc.ucuenca.areaconocimientounescoespecifico | 091 - Salud | |
dc.ucuenca.areaconocimientounescodetallado | 0912 - Medicina | |
dc.ucuenca.urifuente | http://www.sciencedirect.com/science/journal/00359203 | |
Appears in Collections: | Artículos |
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