Identificación de actividad enzimática de fosfatasa en muestras de agua y lodo de la planta de tratamiento de aguas residuales de Ucubamba de la ciudad de Cuenca

Abstract

The industrial, agricultural, pharmaceutical and domestic operations are sources of pollution to natural environments (soil, water, air) with heavy metals. This contamination produces loss of biodiversity, decrease of socio-economic activities and increase in diseases. Different studies have reported that phosphatase enzymes can remove heavy metals, so, this research looked for proteins with phosphatase enzymatic activity from wastewater and sewage of the Wastewater Treatment Plant of Ucubamba in the Cuenca city. The histochemical screening of metagenomics libraries built from PCR products of acid phosphatase (PhoN) cloned in the pET302/NT-His vector, expressed in E.coli BL21 (DE3) Start were carried out. For being identified phosphatase activity used an indicator medium LB-agar supplemented with phenolphthalein diphosphate and methyl green. With the above was achieved detecting enzymatic phosphatase activity in all the samples of wastewater and sewage, being more frequent in clones from wastewater de maturation lagoons.