Remoción de la hemoglobina por adsorción sobre nanopartículas magnéticas de Fe3O4/TiO2

Abstract

The removal of Hemoglobin (Hb) by protein adsorption on magnetic nanoparticles (Nps) of Fe3O4/TiO2 (core/shell), applying an external magnetic field, was investigated using ultraviolet spectroscopy in the ultraviolet and visible range (UV-VIS). The Nps concentration and temperature was varied during the contact step between Nps and Hb. Complementary, the intensity of the external magnetic field was varied during the removal of the Fe3O4/TiO2-Hb complex. With an initial Nps concentration of 8 mg ml-1, the absorbance of the supernatant solution showed a decrease of 11% with respect to the initial Hb solution (0,8 g l-1), and a decrease of 17% when the Nps concentration was increased with 50%. A reduction of the absorbtion of Hb was observed by increasing the temperature from 25 to 35ºC, but no evidence of denaturalization was found. During the Fe3O4/TiO2-Hb complex removal step, the absorption intensity nearly decreased 30% by increasing the strength of the magnetic field. Finally, the biochemical oxygen demand (BOD5) of the initial Hb solution and supernatant was determinated. The value of the initial Hb solution was 100 mg ml-1 and the results for all treated solutions were lower (< 62 mg ml-1) reflecting the impact of magnetic Nps in the removal of Hb