Determinación de inmunoglobulina A en leche materna en los quince días posparto

Abstract

The present study has been done in order to quantify the concentration of immunoglobulin A in breast milk during the fifteen days postpartum, consequently covering the three phases of lactation: colostrum, transitional and mature. The research design through observation and cross sectional descriptive, in five volunteer mothers who delivered at term and nursing diagnosis admitted with labor and pregnancy over 38 weeks at the Foundation Humanitarian "Pablo Jaramillo Crespo” Hospital. Breast milk samples were collected during postpartum period (24 hours) until two postpartum weeks later. Seventy-five breast milk samples were processed in duplicate 5 lactating mothers; determining secretory IgA concentrations using the technique of radial immunodiffusion (RID). It was found that IgA concentration has its maximum concentration on the first day postpartum (407.47 mg/dL), if compared to the fifteenth day, where the volume of milk is higher, but it has lower concentration of IgA (55.93 mg/dL), showing the validity of the data obtained by a box diagram. We also carried out a statistical analysis ANOVA for the variables of parity and type of delivery, the results obtained indicate that neither variables influence the IgA concentration during the 15 days postpartum, parity (p=0.432), and mode of birth. (p=0.842).