Browsing by Author "Wilches Arizabala, Isabel Maria"

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Anti-inflammatory and sedative activities of Peperomia galioides: in vivo studies in mice
(2019) Clos, Victoria; Jiménez Altayó, Francesc; Peñaherrera Wilches, María Eugenia; Jerves Andrade, María de Lourdes; Tobar Solano, Vladimiro Alexis; Vander Heyden, Yvan; León Tamariz, Fabián; Vila Calsina, Elisabet Null; Wilches Arizabala, Isabel Maria; Jimenez Castillo, Priscilla Yazmin; Cuzco Quizhpi, Nancy Mirian
© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. Aerial parts (leaves, flowers, stem) of Peperomia galioides extract administered to mice, was used to confirm its anti-inflammatory and sedative folk uses. The anti-inflammatory activity was assessed by croton oil-induced ear oedema and myeloperoxidase (acute inflammation); cotton pellet-induced granuloma (sub-acute inflammation) and Escherichia coli Lipopolysaccharide (LPS) induced inflammation (cellular mediators). The sedative activity was studied by the pentobarbital-induced sleeping time test. Single doses (300 and 600 mg/kg; i.p.) of the extract reduced croton oil-induced ear oedema and myeloperoxidase activity. Six days administration of the extract (300 mg/kg, i.p.) to mice implanted with cotton pellets diminished granuloma formation. LPS (20 mg/kg, i.p.) enhanced plasma nitrites and TNF-α levels that were inhibited by the extract. The duration but not the onset of sleeping time was enhanced by 300 and 600 mg/kg of the extract. Our results show that P. galioides has anti-inflammatory and sedative activities in mice, which validates its traditional use.
Anti-inflammatory and sedative activities of Peperomia galioides: in vivo studies in mice
(2019) Vander Heyden, Yvan; Tobar Solano, Vladimiro Alexis; Jerves Andrade, María de Lourdes; Peñaherrera Wilches, María Eugenia; Jiménez Altayó, Francesc; Clos, Victoria; León Tamariz, Fabián; Vila Calsina, Elisabet Null; Wilches Arizabala, Isabel Maria; Jimenez Castillo, Priscilla Yazmin; Cuzco Quizhpi, Nancy Mirian
© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. Aerial parts (leaves, flowers, stem) of Peperomia galioides extract administered to mice, was used to confirm its anti-inflammatory and sedative folk uses. The anti-inflammatory activity was assessed by croton oil-induced ear oedema and myeloperoxidase (acute inflammation); cotton pellet-induced granuloma (sub-acute inflammation) and Escherichia coli Lipopolysaccharide (LPS) induced inflammation (cellular mediators). The sedative activity was studied by the pentobarbital-induced sleeping time test. Single doses (300 and 600 mg/kg; i.p.) of the extract reduced croton oil-induced ear oedema and myeloperoxidase activity. Six days administration of the extract (300 mg/kg, i.p.) to mice implanted with cotton pellets diminished granuloma formation. LPS (20 mg/kg, i.p.) enhanced plasma nitrites and TNF-α levels that were inhibited by the extract. The duration but not the onset of sleeping time was enhanced by 300 and 600 mg/kg of the extract. Our results show that P. galioides has anti-inflammatory and sedative activities in mice, which validates its traditional use.
Anticonvulsant activity of bisabolene sesquiterpenoids of Curcuma longa in zebrafish and mouse seizure models.
(2012) Orellana Paucar, Adriana Monserrath; Serruys, Ann Sophie K.; Afrikanova, Tatiana; Maes, Jan; De Borggraeve, Wim; Alen, Jo; León Tamariz, Fabián; Wilches Arizabala, Isabel Maria; De Witte, Peter A.M.; Esguerra, Camila V.
urmeric, obtained from the rhizomes of Curcuma longa, is used in South Asia as a traditional medicine for the treatment of epilepsy. To date, in vivo studies on the anticonvulsant activity of turmeric have focused on its principal curcuminoid, curcumin. However, poor absorption and rapid metabolism have limited the therapeutic application of curcumin in humans. To explore the therapeutic potential of turmeric for epilepsy further, we analyzed its anticonvulsant activity in a larval zebrafish seizure assay. Initial experiments revealed that the anticonvulsant activity of turmeric in zebrafish larvae cannot be explained solely by the effects of curcumin. Zebrafish bioassay-guided fractionation of turmeric identified bisabolene sesquiterpenoids as additional anticonvulsants that inhibit PTZ-induced seizures in both zebrafish and mice. Here, we present the first report of the anticonvulsant properties of bisabolene sesquiterpenoids and provide evidence which warrants further investigation toward the mechanistic understanding of their neuromodulatory activity.
Evaluation of the variables altitude, soil composition and development of a predictive model of the antibacterial activity for the genus Hypericum by chromatographic fingerprint
(2019) Ortega Puma, Carmen Maribel; Fajardo Carmona, Sandra Isabel; Ortiz Ulloa, Silvia Johana; Tobar Solano, Vladimiro Alexis; Quito Ávila, Diego; Santos Ordóñez, Efrén; Jerves Andrade, María de Lourdes; Cuzco Quizhpi, Nancy Mirian; Wilches Arizabala, Isabel Maria; León Tamariz, Fabián
H. laricifolium Juss is a native shrub from the Andes used for the treatment of antibacterial infections among other illnesses. Nowadays, due to the growing need of new antibiotic substances, this medicinal plant would represent an important source for the investigation of antibacterial activity due to the wide medicinal potential of its genus, property that can be analyzed through its chemical fingerprint profile. The objectives of this research were, to develop a predictive model for the antibacterial potential of the genus Hypericum using chromatographic fingerprinting with three Hypericum species collected at Ecuadorian Andes (H. laricifolium, H. quitense R. Keller and H. decandrum Turcz); and to evaluate the influence of natural variables such as altitude and soil composition on the pharmacological effect of lipophilic extracts of H. laricifolium. Antibacterial activity of the plants extracts was assessed by plate microdilution technique against Methicillin-resistant Staphylococcus aureus (MRSA). The fingerprint was obtained by HPLC-DAD. The predictive model was constructed from the chromatographic fingerprint of H. laricifolium and H. quitense and their IC50 values using the “Partial least squares” multivariate analysis tool. Using the regression coefficients of the model, the predictive equation of antibacterial activity for new samples of H. decandrum was established. The extracts of H. laricifolium exhibited a high antibacterial activity with the pH and the organic matter as main factors influencing the activity. The prediction model obtained did not have predictive ability to different Hypericum species, which could be explained by differences in the chemical composition of the three species.
Impact of nutrient stress on antioxidant production in three species of microalgae
(2015) León Tamariz, Fabián; Goiris, Koen; Van Colen, Willem; Wilches Arizabala, Isabel Maria; Coomana, Luc de; Muylaert, Koenraad
Microalgae are a novel source of sustainable natural antioxidants with various applications, including food preservation. To optimize antioxidant production in microalgae, we investigated the influence of nutrient limitation on antioxidant content in three species, Phaeodactylum tricornutum, Tetraselmis suecica and Chlorella vulgaris. Microalgae were cultivated in batch culture under nutrient replete, P- and N-limited conditions. Total antioxidant activity of the biomass was measured using Trolox equivalent antioxidant capacity and square wave voltammetry. Additionally, contents of carotenoids, phenolics, tocopherols and ascorbic acid were measured. Nutrient limitation, particularly N-limitation, resulted in low antioxidant content. Both phenolic and carotenoid contents were significantly reduced in nutrient-limited cultures. In contrast tocopherols and ascorbic acid levels were higher in nutrient-limited cultures, particularly under P-limitation. Our results indicate that nutrient stress is not an effective strategy to enhance overall antioxidant content in microalgae, although it may be useful to enhance production of some vitamin antioxidants such as tocopherols or ascorbic acid. © 2014 Elsevier B.V.