Browsing by Author "Wilches Arizábala, Isabel María"

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Comparación de la aplicabilidad de las técnicas DPPH (1,1-difenil-2-picril-hidrazilo) y Peróxidos para extractos resinosos y no resinosos en metanol y diclorometano
(Universidad de Cuenca, 2015-06-02) Espinoza Castro, Karla Esther; López Domínguez, María Belén; Wilches Arizábala, Isabel María
In the present research, the antioxidant capacity present in methanol and di-chloro methane extracts obtained from leaves of Jungia rugosa Less and Desmodium molliculum was evaluated. Desmodium molliculum, a plant traditionally named “hierba del infante” was collected into the Azuay province and was botanically characterized at Herbarium Azuay (HA) into the Universidad del Azuay. Dried extracts were obtained by means of percolation, using methanol and dichloromethane as solvents for extraction. A fraction of those extracts was used for their phytochemical characterization using a general screening method based on Thin Layer Chromatography (TLC). Results show the presence of phenolic compounds (flavonols and flavone) and terpenoids as the more important compounds. To evaluate antioxidant activity, two different techniques were applied: discoloration of radical 2,2-diphenyl-1-picrilhidrazilo (DPPH) and the hydrogen peroxide scavenging assay, ascorbic acid was used as a standard, IC50 value for standard was determined as 2.68 ug/ml by the first method and 58.63 ug/ml by the second method. In the DPPH assay, IC50 value for methanolic extracts of Jungia rugose Less was between 40 – 129 µg/ml and for dichloromethane extracts was between 74 – 340 µg/ml. IC50 of Desmodium molliculum was 48 µg/ml in methanolic extract and 80 ug/ml in dichloromethane extract. When hydrogen peroxide assay was developed, the IC50 value of Desmodium molliculum methanolic extract was 61 µg/ml, and between 177 – 349 µg/ml for Jungia rugosa Less. Antioxidant activity of dichloromethane extracts was not evaluated by this technique due to incompatibility with the reagents used. The findings suggest that “Jungia” and “Desmodium” have antioxidant activity, which could be related to the presence of phenolic compounds.
Correction to: Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils
(2022) Vanegas Jácome, David Enrique; León Tamariz, Fabián; Calle Lopez, Jessica Priscila; Wilches Arizábala, Isabel María; Cuzco Quizhpi, Nancy Mirian; Peñaherrera Wilches, María Eugenia; Jerves Andrade, María de Lourdes; Khachatryan , Aleksandr; Abril Novillo, Andrea Soledad
Following the publication of the original article [1] we were informed that the authors’ given and family names had unfortunately been interchanged. The author names have been corrected in the author list of this Correction and updated in the original article.
Cuantificación de material particulado PM10 y su efecto toxicológico-ambiental, en la ciudad de Azogues
(2014) Vivar Martínez, Edgar Fabián; Wilches Arizábala, Isabel María
For more than 30 years the city of Azogues has suffered from contamination by dust, however have not been studies that evaluate technically the degree of pollution. This study raised main objective contribute to the evaluation of the quality of the air in the city of Azogues, through quantification of the concentrations of material particulate matter PM10 in two parts of the city through surveys with the sampler of high-volume (High Volume Sampler, HVS), using micro quartz fiber filters, alternating every four days and following procedures established by the Ecuadorian legislation. The study was conducted during the months between September 2013 to March 2014. Average daily PM10 concentrations during the six months of monitoring was 70.3 µg/m3, while Ecuador 100 µg/m3 air quality standard, it does not exceed exceeding Yes the one given by the World Health Organization that establishes the maximum limit of 50 µg/m3, the population would be exposed to the development of some conditions especially of respiratory type. The concentration of material particulate matter PM10 exceeded the Ecuadorian legislation in 14% of the determinations made and 70% of the who standard. Unfortunately, the negative effects on the health of the inhabitants are not being evaluated by any institution.
Desarrollo galénico de un vehículo tipo emulsión para el protector solar eusolex 4360
(2006) Fajardo Muñez, María Eufrasia; Wilches Arizábala, Isabel María
Desarrollo galénico de un vínculo tipo emulsión para el protector solar eusolex 4360
(2006) Cuzco Quizhpi, Nancy Mirian; Wilches Arizábala, Isabel María
Determinación de la actividad antimicrobiana de jungia rugosa less en extractos de n-hexano y diclorometano
(2014) Solís Bowen, Sara Bethzabé; Wilches Arizábala, Isabel María
Jungiarugosa Less is a native plant from Ecuador, its leaves had been used since ancient age for people from Andes due to the anti-inflammatory and cicatrizing effects that this medicinal plant has showed. This study was conducted to determine the antibacterial activity from extracts of leaves, flowers and stems of Jungiarugosa Less in dichloromethane and n-hexane against bacteria such us Staphylococcus aureus(ATCC 25923), Escherichia coli (ATCC 25922 )and Salmonella typhimurium(ATCC 14028). The extracts were obtained by percolation and concentrate under vacuum. TLC (Thin Layer chromatographic) method was performed determining the presence of flavonols and flavones only in the leaves extracted with dichloromethane, and the presence of terpenoid compounds and coumarins in leaves, flowers and stems extracted with dichloromehane and n-hexane. To determinate the antibacterial activity three different technique were applied: microdilution plate, dilution plate and agar diffusion, however it was not possible to obtain results about the antibacterial activity by the lack of solubility of the extracts, due to its high resinous character which resulted in a lack of reproducibility of the results.
Determinación del metabolito tóxico de origen fúngico en leche cruda, pasteurizada y ultrapasteurizada consumidas en la ciudad de Cuenca: aflatoxina AFM1 mediante la técnica de elisa
(2013) Uguña Rosas, María Fernanda; Wilches Arizábala, Isabel María
At present there is great concern of health agencies worldwide for food toxicology issues and pollution that has food by toxic metabolites such as aflatoxin AFM1 which is a latent risk in different health conditions such as immunosuppressive synthesis processes interrupting the formation of DNA and RNA causing relentless mutagenic, teratogenic and carcinogenic further liver damage in animals, and group 1 carcinogen to humans , with an alarming problem that needs to be analyzed from the nutritional , toxicological , socioeconomic , environmental, scientific and control. This study was conducted in order to determine quantitatively the content of aflatoxin AFM1 in three types of raw milk, pasteurized and ultra pasteurized consumed in the city of Cuenca, by ELISA technique and compare the results obtained with the maximum parameters referred internationally FDA (Food and Drug Administration of the United States of America) , FAO (Food and Agriculture Organization ) and current national regulations INEN ( Ecuadorian Standardization Institute ) . Of the samples tested any exceeded the detection limit of the technique ( 125 ppt or 0.125 ppb ) , which is below the maximum limits established by national law both INEN 9-10 force and the parameters set by FAO and FDA (0.5 ppb). Therefore, the concentration of aflatoxin AFM1 in milk analyzed by ELISA does not contain levels constituting a health risk to the consumer population.
Determinación química de Hemoglobina Glicosilada
(1991) Paz Cueva, Kyra; Donoso Moscoso, Silvana Patricia; Wilches Arizábala, Isabel María; Flores García, Gil
Diseño de un gel bucal de propóleos
(2005-11-11) Crespo Ruiz, María Viviana; Farfán Lima, Mónica Cristina; Wilches Arizábala, Isabel María
Estudio de la migración del ftalato di (2-etilhexil) ftalate (DEHF) en botellas PET al agua embotellada consumida en la ciudad de Cuenca
(2019-06-12) Fajardo Muñoz, María Eufrasia; Wilches Arizábala, Isabel María
The quality and safety of food products is conditioned by the behavior of the containers, because the food interacts with the container and the surrounding environment, the migration of phthalates to food, is a toxicological risk to human health, phthalates are endocrine and carcinogenic disruptors; such as di (2-ethylhexyl) phthalate (DEHF), due to its high exposure and toxicity in animals and humans. (Al Saleh et al., 2011). In Ecuador, no processes of good storage practices have been implemented, so the containers are exposed to conditions that favor the release of toxic phthalates, for this reason in this study the migration of the DEHF was investigated in 24 samples of bottled water from the brand with the highest consumption in the city of Cuenca, which is distributed and marketed in three supermarkets, selected according to surveys carried out. The extraction of the sample is liquid-liquid (LLE) and the analytical technique used is Gas Chromatography (GC). In this study, 3 samples showed DEHF migration at concentrations of (0.001850 mg/l, 0.001676 mg/l and 0.001294 mg/l), values that are below the parameter established by the Ecuadorian Technical Regulations NTE INEN 2200: 2017 , NTE INEN 1108 and according to the requirements of the WHO that refers a maximum value of (0.008 mg/l), so it is considered that the analyzed samples of bottled water in PET container, of the brand selected in the supermarkets of The city of Cuenca does not constitute a danger to the consumer.
Estudio Farmaco-Técnico Comparativo de Comprimidos Genéricos de Furosemida (40 mg.) tomando como referencia al innovador Lasix
(2005) Abril Espinoza, Jéssica Paola; Loayza Coronado, Roberth; Wilches Arizábala, Isabel María
Evaluación de la actividad antioxidante de extractos metanólicos y de diclorometano de Jungia paniculata, Jungia coarctata, Jungia fistulosa y Jungia rugosa usando la técnica DPPH y el poder reductor férrico
(2015-10-21) Aguilar González, Karen Jhovana; Tenorio Arévalo, María Caridad; Wilches Arizábala, Isabel María
In this research the antioxidant capacity from Jungia rugosa, Jungia coarctata, Jungia paniculata and Jungia fistulosa leaves was evaluated using DPPH (2, 2 -diphenyl-1-pycrylhidrazyl) and reducing power (reduction of ferricyanide) techniques. DPPH assay evaluates bleaching radical (DPPH) by action of an antioxidant and technique ferric reducing power the capacity of a reducing agent to react with potassium ferricyanide and produce potassium ferrocyanide. Ascorbic acid was used as standard wich IC50 was 3.07 µg/ml in the art of DPPH. In the DPPH test, the evaluation of the antioxidant activity from the extracts and the DCMs, was carried out using the IC50 (concentration to which 50% of free radicals are inhibited), obtaining values between 71,09-352,86 µg/ml µg/ml for methanol and between 7,55-182,29 µg/ml for DCM. For the reducing power test, the evaluation of the antioxidant activity from the extracts and the DCMs, was carried out by the AAEAC (milligrams of ascorbic acid equivalent to 1 gram of dry drug), obtaining values between 6, 58-271,09 mg/g for methanol and between 2,05-72,29 mg/g for DCM. Through an ANOVA test and a Tukey test it was proved that there are differences in the antioxidant capacity between species in both antioxidant techniques. Using a thin layer chromatography, the identification of metabolites of different species of Jungia was performed, finding the phenolic compounds as the main substances in granting the antioxidant properties of these plants. The findings suggest that the extracts analyzed from species Jungia have antioxidant activity, attributed to the presence of phenolic compounds and terpenes.
Evaluation of anti-inflammatory activity of the methanolic extract from Jungia rugosa leaves in rodents
(2015) Wilches Arizábala, Isabel María; Tobar Solano, Vladimiro Alexis; Peñaherrera Wilches, María Eugenia; Cuzco Quizhpi, Nancy Mirian; Jerves Andrade, María de Lourdes; Vander Heyden, Yvan; León Tamariz, Fabián; Vila Calsina, Elisabet Null
Ethnopharmacological relevance: Jungia rugosa Less (Asteraceae), popularly known in Ecuador as "Carne humana" or "Fompo", is a vine present into the Andean region. It is traditionally used as medicine for the treatment of bruises, cuts and other external inflammatory processes. This study was designed to investigate the anti-inflammatory activity of J. rugosa leaves extract (JRLE) in rodents. Material and methods: The acute anti-inflammatory activity was evaluated by animal models, including croton oil-induced ear oedema in mice, carrageenan-induced paw oedema in rats and myeloperoxidase (MPO); the chronic anti-inflammatory activity was evaluated by cotton pellet-induced granuloma. Results: Intraperitoneal administration of JRLE (125, 250, 500 mg/kg) significantly (p<0.01-0.001) inhibited the croton oil-induced ear oedema and MPO activity in mice; the carrageenan-induced paw oedema in rats was significantly (p<0.05) reduced by 500 mg/kg. Repeated (6 days) administration of the extract to mice previously implanted with cotton pellets reduced the formed granuloma (125 mg/kg: 11.7%; 250 mg/kg: 17.9%; 500 mg/kg: 32.4%) but only the inhibition by 500 mg/kg reached statistical significance (p<0.01). Conclusions: The results show that JRLE is effective as an anti-inflammatory agent in acute and chronic inflammation in mice, supporting its traditional use. © 2015 Elsevier Ireland Ltd. All rights reserved.
Optimization and pharmacological validation of a leukocyte migration assay in zebrafish larvae for the rapid In vivo bioactivity analysis of anti-inflammatory secondar metabolitesy
(2013) Cordero Maldonado, María Lorena; Siverio Mota, Dany; Vicet Muro, Liliana; Wilches Arizábala, Isabel María; Esguerra, Camila V.; Witte, Peter A. M. de; Crawford, Alexander D.
Over the past decade, zebrafish (Danio rerio) have emerged as an attractive model for in vivo drug discovery. In this study, we explore the suitability of zebrafish larvae to rapidly evaluate the anti-inflammatory activity of natural products (NPs) and medicinal plants used in traditional medicine for the treatment of inflammatory disorders. First, we optimized a zebrafish assay for leukocyte migration. Inflammation was induced in four days post-fertilization (dpf) zebrafish larvae by tail transection and co-incubation with bacterial lipopolysaccharides (LPS), resulting in a robust recruitment of leukocytes to the zone of injury. Migrating zebrafish leukocytes were detected in situ by myeloperoxidase (MPO) staining, and antiinflammatory activity was semi-quantitatively scored using a standardized scale of relative leukocyte migration (RLM). Pharmacological validation of this optimized assay was performed with a panel of anti-inflammatory drugs, demonstrating a concentration-responsive inhibition of leukocyte migration for both steroidal and non-steroidal anti-inflammatory drugs (SAIDs and NSAIDs). Subsequently, we evaluated the bioactivity of structurally diverse NPs with well-documented antiinflammatory properties. Finally, we further used this zebrafish-based assay to quantify the anti-inflammatory activity in the aqueous and methanolic extracts of several medicinal plants. Our results indicate the suitability of this LPS-enhanced leukocyte migration assay in zebrafish larvae as a front-line screening platform in NP discovery, including for the bioassayguided isolation of anti-inflammatory secondary metabolites from complex NP extracts.
Preparación de extractos vegetales: determinación de eficiencia de metódica
(2010) Carrión Jara, Ana Victoria; García Gómez, Cándida Rafaela; Wilches Arizábala, Isabel María
Relación entre el contenido de fenoles totales y la actividad antioxidante de Jungia rugosa Less en extractos metanólicos y de diclorometano
(2015-01-15) Cambizaca Heredia, Liz Natalia; Bermeo Guerrero, Carmen Maribel; Wilches Arizábala, Isabel María
Reactive oxygen species (ROS), including radicals can generate oxidative stress, a phenomenon which is attributed many neurodegenerative diseases. It has been shown that plants and their constituents are a source of antioxidants; thus they have the capacity to capture EROS. In this paper the antioxidant activity of extracts from leaves rough Less Jungia was evaluated by DPPH techniques and reducing power in the determination of phenolic compounds Ciolcateau Folin technique was applied. Furthermore, the phytochemical composition was determined using chromatographic fingerprint method as chromatography and thin layer (TLC).The results obtained showed the presence of flavonoid type flavonols and flavones, coumarins, possibly terpenoid saponins. In the analysis of antioxidant activity two techniques were used: the reduction of 1-2,2-diphenyl picrilhidrazilo radical (DPPH) and the reducing power, for which worked with ascorbic acid as standard a value of IC50 obtained 2,90 ug / ml in the method of DPPH and 4.71 ug / ml for the technique of reducing power. In the technique of DPPH IC50 value of the methanol extracts of rough Less Jungia he was found in a range of 31.45 -80.25 ug/ml and dichloromethane between 128,54- 262.13 ug/ml. In developing the technique of reducing power, IC50 of methanol extracts it varies between a range of 33.33 -166.63 mg / ml and dichloromethane extracts from 11.47 -21.74 mg / ml. In determining the phenolic compounds by the technique of Folin Ciolcateau concentrations between 0,81-3.39 mg / ml were obtained in the methanol extract, with regard to the dichloromethane extracts could not be evaluated because of incompatibility with the reagent employee