Browsing by Author "Vizhco Yupangui, Gabriel Leonardo"

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    Autovacuna para Staphylococcus aureus como estrategia de control de mastitis subclínica en bovinos
    (Universidad de Cuenca. Facultad de Ciencias Agropecuarias, 2026-02-11) Chauca Sanpedro, Joseph Pablo; Vizhco Yupangui, Gabriel Leonardo; Maldonado Rivera, Jaime Eduardo
    Subclinical bovine mastitis, common in dairy herds, reduces milk quality and volume, causing economic losses; autovaccines are proposed as an alternative control measure. In this study, an autovaccine against Staphylococcus aureus was formulated and administered to cows with subclinical mastitis on the Irquis and Nero farms. The vaccine was obtained from milk samples from animals, which were subjected to isolation, identification, multiplication, and attenuation of the pathogen, and combined with adjuvants. Five cows received three doses over 60 days. The evaluation included the California Mastitis Test (CMT), colony-forming unit (CFU) count, and somatic cell count (SCC). No inhibition halo tests or in vitro antimicrobial sensitivity assays were performed, so the results were limited to cellular and bacteriological parameters in milk. The analyses showed significant differences in cell migration after vaccination, reflected in increased CMT (p=0.026) and SCC (p=0.029) reaction grades, with values above 1,500,000 cells in 60% of cases. The CFU count did not show statistical differences (p=0.118), although reductions were observed in some animals. In conclusion, the autovaccine stimulated the cellular response in the mammary gland and contributed to reducing the bacterial load in individuals. These findings indicate that the autovaccine could be a complementary tool in the control of bovine mastitis, but a larger sample size, longer follow-up, and additional testing are required to consolidate the scientific evidence.