Browsing by Author "Villena, Paulina G."

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Diversidad genética de accesiones de la familia Caricaceae en el sur de Ecuador
(Universidad de Cuenca, 2017) Peña, Denisse F.; Villena, Paulina G.; Aguirre, Ángel J.; Jiménez Merino, Carlos
The genetic diversity of 78 accessions of the Caricaceae family collected in the provinces of Azuay, Cañar, Loja, El Oro and Zamora Chinchipe, south of Ecuador, was studied, including the species Vasconcellea candicans, V. goudotiana, V. longiflora, V. microcarpa, V. monoica, V. omnilingua, V. palandensis, V. stipulata, V. parviflora, V. sp., V. x heilborne, V. x heilborne cv pentagona, V. x heilborne cv chrysopetala, Carica papaya, Jaracatia digitata y J. spinosa by DNA amplifications using 14 aleatory primers from AA, AC, AM and AN series of Operon Technologies Inc. The used RAPD primers produced 76 polymorphic fragments with sizes between 368 and 2102 base pairs (bp). The cluster and principal coordinate analysis grouped the accessions of each kind among them and the results were consistent with previous studies. The dendrogram separated clearly the three genera, however, based on these results, studies and morphological observations, the creation of a new genera that groups the Vasconcellea candicans accessions is suggested. Furthermore, the authors support the hypothesis of designating the materials collected in the Molleturo zone as a new taxón of the genera Vasconcellea. As in other studies, the results suggest possible events of interspecific hybridization within Vasconcellea genus.
Evaluación de los sistemas de cultivo semisólido y BIT en la multiplicación in vitro de Juglans neotrópica
(Universidad de Cuenca, 2017) Rocano, Mélida N.; Villena, Paulina G.; Peña, Denisse F.
The purpose of this study was to evaluate two culture systems, semi-solid and temporary immersion in bioreactors (BIT), for the micropropagation of Juglans neotropica, using zygotic embryos. Additionally, in the BIT culture system also the effect of the frequency of immersion and forced aeration on the micropropagation was examined. The culture growth of the shoots was measured by the following characteristics: number and size of the shoots, number of leaves, and the wet and dry weight. No statistical differences were detected. In general, the BIT immersion system produced an equivalent number of plants of similar characteristics as the embryos propagated in the traditional semi-solid medium method. However, BITs produced more shoots, forced ventilation resulted in less hyperhydric shoots and the BIT system was considerable cheaper, resulting in an average production cost of $0.21 per plant versus $0.40 in the semi-solid propagation method.