Browsing by Author "Valverde Peralta, Edwin Enrique"

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    Efecto de dos temperaturas de almacenamientos (5 y 20 °C) de epidídimos provenientes de toros de matadero sobre la calidad y congelabilidad de espermatozoides
    (2016) Valverde Peralta, Edwin Enrique; Galarza Lucero, Diego Andrés
    In this research we evaluated the sperm quality post-thawing of sperm of the cauda epididymis of bulls of slaughterhouse subject to two temperatures of storage (5°C and 20°C). 8 pairs of testicles was collected stored tightly in plastic bags with Lactate Ringer for 6 hours. The gametes were recovered by retrograde flow of the cauda epididymis sperm concentration, viability was assessed and processed by adjusting doses of 50 x 106 sperm / 0.25ml with the diluent AndroMed to 20%. It packing, cryopreserved in liquid nitrogen. At 7 days assessed quality post-thawing with a conventional method and with a computerized system (CASA) Sperm Vision Production- versión 1.01 (Minitube). The data were analyzed in the SPSS program (version 22) obtaining best results to 5°C a 84,2 ±1,54 % in recovered sperm, there are significant differences (p<0,05) according to the ADEVA. In quality post-thawing also showed better results of MIP, vitality sperm (VE) with 5°C, 33,4±2,42 and 60,3±2,61 %, respectively, with significant differences (p<0,05 ) but with the CASA system to evaluate IPM there was no differences (p<0,05 ), however, morphologic abnormalities the two groups were similar (p<0,05 ); and in the test of endosmosis of the membrane (HOST) was obtained best results with 20° C with 40,4±3,26 (p<0,05). We corroborate this statistical relationship with a multivariate analysis (MANOVA) by grouping variables and analyzing in the study groups (p>0,05). In conclusion, quality post-thawing was different in the two groups but at 20°C storage was obtained results lower sperm quality post-thawing rejecting the issues raised, except for of the evidence of HOST
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    Efecto de la temperatura de almacenamiento de epidídimos de toro sobre la calidad espermática de semen criopreservado
    (2018) Valverde Peralta, Edwin Enrique; Argudo Garzon, Daniel Ernesto; Bueno Leon, Hernan Patricio; Quintero Moreno, Armando; Galarza Lucero, Diego Andrés
    Epididymis sperm conservation is a technique widely used in animals of high genetic value as well as in wild animals dead unexpectedly. One factor that most influence in motility and sperm membrane integrity (plasma and acrosomal) as well as the fertilizing ability of epididymal spermatozoa recovered is post-mortem temperature storage of epididymis. The objective of this study was determinate sperm quality pre-freezing and post-thawed of bull epididymal sperm (Bos taurus) from the Ecuadorian Amazon recovered from testicles stored to 5 or 20ºC for short-time. For this propose, ten pairs of testicles were collected from slaughterhouse. Left and right testicles of each bull were storage at 5ºC (T-5 group) or 20ºC (T-20 group), respectively during 6 hours (h). Sperm recovery was performed by retrograde flow of the epididymis tail and the liquid obtained was diluted in AndroMed® extender at concentration of 200 x 106 spermatozoa/ mililiters mL and packed in 0.25 mL straws. Both straws T-5 (n = 30) and T-20 (n = 30) groups were frozen in vapors of nitrogen liquid and thawed at 37ºC for 60 seconds (sec). The effect of storage temperature of epididymis post-mortem were assessment by ANOVA-one way. The results showed that in pre-freezing analysis, the percentage of motility (analyzed by optical microscopy), sperm vitality (V, plasma membrane integrity analyzed by eosin- nigrosine stain) and total morphological abnormalities (TA) of spermatozoa of T-5 group did no showed significant differences (P>0.05) compared with T-20 group. In post-thawed analysis, likewise the motilities variables (assessed by CASA system, SpermVision®, Minitube, Germany) as well as TA did no showed significant differences (P>0.05) between T-5 and T-20 groups. Sperm vitality (V) in samples thawed was higher (P<0.05) with T-5 group than T-20 group (63.0 ± 2.6% y 52.9 ± 3.0%, respectively). However, in plasma membrane functionality analysis assessed by hypoosmotic test (HOST) showed higher values (P<0.05) with T-20 group than T-5 group (40.4 ± 3.26% and 31.3 ± 1.99%). In conclusion, the results suggest that, despite the controversies found in the integrity and functionality plasma membrane post-thawed, the epididymis from Bull slaughtered under environment conditions of Ecuadorian Amazon may be storage at 5 or 20ºC during short-term. Additional studies should be made to evaluate the fertilizing ability of these epididymal sperm after artificial insemination (AI) under filed conditions.