Browsing by Author "Uriguen Zumba, Erik Andres"

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    Evaluación del rendimiento diagnóstico del ELISA indirecto en suero sanguíneo y suero lácteo para la detección de Brucella spp. en bovinos del cantón Sevilla de Oro
    (Universidad de Cuenca. Facultad de Ciencias Agropecuarias, 2026-02-12) Uriguen Zumba, Erik Andres; Ramónez Cárdenas, Juan Carlos
    Brucellosis is a globally distributed zoonotic disease that causes significant economic losses in livestock production. Its diagnosis relies on laboratory tests applied to both blood serum and milk, which exploit the antigenic properties of the O antigen present in the lipopolysaccharide of the cell envelope of Brucella spp. In this context, the objective of this study was to evaluate the diagnostic performance of indirect ELISA applies to blood serum and milk serum for the detection of Brucella spp., antibodies in lactating cattle. A total of 86 paired samples per matrix (172 samples in total) were analyzed. The apparent prevalence estimated using blood serum was 15,12%. ELISA-I performed on milk showed a sensitivity of 92,32% and a diagnostic specificity of 97,26% with positive and negative predictive values of 85,71% and 98,61%, respectively, which is consistent with the low prevalence observed and reinforces the reliability of negative results. Agreement between both matrices was almost perfect (kappa= 0,868; p<0,001), while McNemar’s test showed no statically significant differences between results obtained from blood serum and milk serum (p=1,000). In addition, ROC analysis demonstrated excellent discriminative ability for both matrices, with area under the curve (AUC) values of 1,000 for blood serum and 0,994 for milk serum. Overall, these findings suppor the use of ELISA-I in mil as valid, practical and less invasive diagnostic alternative for the detection of Brucella spp., with potential application in epidemiological surveillance programs without compromising diagnostic accuracy.