Browsing by Author "Supliguicha Merchan, Juan Daniel"

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    Efecto de la GnRH, benzoato de estradiol y ablación folicular en el tiempo de reinicio de la nueva onda folicular en vaconas Holstein Mestiza en fase luteal
    (2019-02-26) Pesantez Duran, Jesús Alejandro; Supliguicha Merchan, Juan Daniel; Narváez Terán, Jhonny Alfredo
    The objective of this study was to evaluate the effect of GnRH, estradiol benzoate and follicular ablation on the restart time of the new follicular wave in Holstein Mestiza vaconas in the luteal phase. The research was conducted in the Nero farm of the University of Cuenca with nine vaconas between 15 and 18 months of age, clinically healthy with a weight greater than 300 kg, and a CC of 3 on a scale of 1 to 5. The synchronization of the luteal phase of the vaconas was performed with the use of an intravaginal progesterone device, estradiol benzoate (BE) and prostaglandin (PGF), 56 hours after removal of the implant, the presence of the pre ovulatory follicle (FPO) was evaluated. On the 9th postapplication of PGF, the presence of a functional corpus luteum and dominant follicle was confirmed, and three treatments were applied, T1 (n = 3) Estradiol benzoate 2.5 mg IM, T2 (n = 3) GnRH 250 ug IM, T3 (n = 3) ablation of the follicles >5 mm in the first period, and in the same way in the following two periods alternating the treatments. To determine the restart time of the new follicular wave, the peaks of FSH in blood were related to the presence of the new cohort of recruited follicles; To measure FSH levels, a blood sample was taken from the jugular vein every 24 hours for 4 days after application of the treatments, and the presence of the new cohort of follicles was evaluated by ultrasound during 4 days post treatments. An experimental 3x3 Latin square design was used with three treatments and three repetitions in different periods. The administration of BE in this study caused the restart of the follicular wave at 3 days post treatment, with the application of GnRH this restart was evidenced at 2.33 days and by follicular ablation, the restart was verified at 2 days. The Duncan test determined that there is a significant difference (p<0.05) in the restart time of the three treatments used, grouping them into different homogeneous subsets. During the two pharmacological treatments, atresia of the dominant follicle was observed