Browsing by Author "Romero Pacheco, Dennis Andres"

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    Evaluación de la respuesta ovárica a la estimulación con dosis baja de hormona foliculoestimulante (FSH), administrada vía epidural previa a la aspiración folicular ecoguiada (OPU)
    (Universidad de Cuenca. Facultad de Ciencias Agropecuarias, 2026-02-12) Angel Quimbay, Xiomara Tatiana; Romero Pacheco, Dennis Andres; Villavicencio Delgado, Luis Antonio; Ayala Guanga, Luis Eduardo
    The research was conducted at the Nero experimental farm of the University of Cuenca, with the aim of evaluating the efficacy of epidural administration of a low dose (125 IU) of follicle- stimulating hormone (FSH) applied in a single administration. Eight heifers were used, initially as a control group (T0) and subsequently as a treatment group (T1), under an intra-subject design. Prior to the start of the experiment, the restart of the follicular wave was synchronized using a protocol that included estradiol benzoate (E2), prostaglandin F2α (PGF2α), and an intravaginal progesterone device (IVD). Day 4 of the protocol was considered the start of the experiment, at which time the T0 group received a placebo, while the T1 group received 125 IU of FSH epidurally. Antral follicle count (AFC) and follicular diameter measurement were performed on days 4, 5, and 6 by ultrasonography. Ultrasound-guided follicular aspiration (OPU) and morphological evaluation of cumulus-oocyte complexes (COC) were performed on day 6. The results showed similar follicular diameters (P > 0.05) on day 4 (T0: 4.0 ± 0.18 mm; T1: 3.9 ± 0.11 mm). However, on day 5, a significant increase in antral follicle size was observed in T1 (5.9 ± 0.20 mm) compared to T0 (4.8 ± 0.16 mm), This trend continued on day 6 (T1: 6.7 ± 0.13 mm; T0: 5.05 ± 0.14 mm). The percentage of oocyte retrieval was higher at T1 (68.5%) than at T0 (53.1%), as was the percentage of COCs suitable for in vitro embryo production (IVEP) (74.2% vs. 56.7%). In conclusion, epidural administration of 125 IU of FSH promoted the development of larger antral follicles, which improved the efficiency and quality of oocyte retrieval by ultrasound-guided follicular aspiration.