Browsing by Author "Romero Aguilar, Bryan Manuel"

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Efecto de ozonoterapia sobre las afecciones uterinas en vacas repetidoras
(2019-11-18) Romero Aguilar, Bryan Manuel; Narváez Terán, Jhonny Alfredo
The research was carried out at “Nero” and “Irquis” farms of the University of Cuenca and the private farm “El Carmen” in the Azuay province; the objective of the study was to evaluate the effect of ozone therapy on subclinical endometritis in repeat breeder cows (RBC), for this study a total of 42 RBC with subclinical endometritis (SE), multiparous with a body condition of 2.5 - 3; the diagnosis of SE was made by endometrial cytology considering positive those that present a percentage ≥ 5% of polymorphonuclear cells (PMN), in addition the diameter of uterine lumen in millimeters (mm) at the base of one of the uterine horns was evaluated by ultrasonography; subsequently, samples of uterine secretion were collected for the identification of bacterial agents; to then perform the intrauterine treatments, for G1 (n = 21) 500 mg of cephapirin benzathine was applied and G2 (n = 21) 45 ug of O3 was used in 50 ml of sterile bidistilled water; after 72 hours the effect of treatments was evaluated. The results were analyzed by basic statisticians, logistic regression and contingency tables, using INFOSTAT. The prevalence of SE in RBC during the study was 47.76%; in the G1 the PMNs decreased from 12.5 ± 0.99 to 6.5 ± 0.76% and for the G2 they showed a greater therapeutic effect 11.5 ± 1.19 to 4.0 ± 0.52%; the diameter of the uterine lumen was reduced in G1 from 2.23 ± 0.14 to 1.72 ± 0.08 and in G2 from 2.21 ± 0.12 to 1.62 ± 0.13 mm not differ between them; in relation to the presence of bacterial agents, 45.24% Staphylococcus spp., 9.5% Escherichia Coli, 7.4% Pseudomonas Spp., 9.5% Bacillus spp., and 38% of the samples did not show isolation bacterial; the percentage of pregnancy reached was 33.0% in G1 and 48.0% for G2 they did not express differences (P> 0.05) but manages to recover fertility. In conclusion, intrauterine administration of benzathine cefapirin and ozone reduces the diameter of the uterine lumen; however, ozone proved to be better in PMN reduction than allopathic treatment (benzathine cefapirin).
Uso de la ozonoterapia intrauterina para tratar la endometritis subclínica en vacas repetidoras de servicios
(2022) Narvaez Teran, Jhonny Alfredo
Subclinical endometritis (SS) is endometrial inflammation usually determined by cytology, in the absence of purulent material in the vagina. In animals without signs of clinical endometritis, the subclinical form of the disease is diagnosed by measuring the proportion of neutrophils present in samples collected by washing the uterine lumen or using a cytobrush. Uterine pathologies play a primary role in reducing reproductive efficiency, with delays in the return of postpartum ovarian activity, increased calving-first service and calving-conception intervals, decreased pregnancy rates from 56% to 30 %, increased number of services per conception and higher culling rates. At present, the different therapeutic treatments such as ozone therapy are focused on improving the intrauterine environment for the survival of the embryo, arousing interest in using alternative therapies to antibiotics applied intrauterinely against uterine infectious processes. The objective of this research was to evaluate the effect of ozone therapy in the treatment of subclinical endometritis in repeating cows