Browsing by Author "Rocano Curillo, Melida Noemi"

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Evaluación de los sistemas de cultivo semisólido y BIT en la multiplicación in vitro de Juglans neotrópica
(2017) Rocano Curillo, Melida Noemi; Villena Ochoa, Paulina Germania; Peña Tapia, Denisse Fabiola
The purpose of this study was to evaluate two culture systems; semisolid and temporary immersion in bioreactors (BIT); for the micropropagation of Juglans neotropica;using zygotic embryos Additionally; in the BIT culture system also the effect of the frequency of immersion and forced aeration on the micropropagation was examined The culture growth of the shoots was measured by the following characteristics number and size of the shoots; number of leaves; and the wet and dry weight No statistical differences were detected In general; the BIT immersion system produced an equivalent number of plants of similar characteristics as the embryos propagated in the traditional semisolid medium method However; BITs produced more shoots; forced ventilation resulted in less hyperhydric shoots and the BIT system was considerable cheaper; resulting in an average production cost of $0.21 per plant versus $0.40 in the semisolid propagation method.
Inducción de la brotación in vitro de microplántulas de nogal (Juglans neotropica) tratadas con Thidiazuron (TDZ) y 6-Bencilaminnopurina (BAP)
(Universidad de Cuenca, 2014-12) Peña Tapia, Denisse Fabiola; Rocano Curillo, Melida Noemi; Salazar, Jazmin M.; Torres, Carlos S.; Universidad de Cuenca; Dirección de Investigación de la Universidad de Cuenca
The walnut (Juglans neotropica) is a species of great potential for conservation projects and eco-agro-production in the Andean region. However, low germination capacity limits its spread for reforestation. The aim of this study was to determine the effect of treatment with Thidiazuron (TDZ) and 6-Benzylaminopurine (BAP) on in vitro shoot induction of J. neotropica. The micro plantlets treated with BAP produced more shoots than those treated with TDZ. The micro plantlets treated with BAP and TDZ in turn generated more shoots than the non-treated micro plantlets. In the test, the effect of concentration of BAP and TDZ in the medium was also evaluated; however not statistically significant differences between the tested concentrations were detected. In summary, findings indicate that the treatment with cytokines, BAP specifically, increases the number of shoots produced by micro plantlets J. neotropica. It is believed that this treatment can be applied on projects of mass propagation of this species within the frame of reforestation programs.
Inducción de la brotación in vitro de microplántulas de Nogal (Juglans neotropica) tratadas con Thidiazuron (TDZ) y 6-Bencilaminnopurina (BAP)
(2014) Peña Tapia, Denisse Fabiola; Rocano Curillo, Melida Noemi; Salazar Orellana, Marjorie Jazmin; Torres Inga, Carlos Santiago
The walnut (Juglans neotropica) is a species of great potential for conservation projects and eco-agro- production in the Andean region. However, low germination capacity limits its spread for reforestation. The aim of this study was to determine the effect of treatment with Thidiazuron (TDZ) and 6-Benzylaminopurine (BAP) on in vitro shoot induction of J. neotropica. The micro plantlets treated with BAP produced more shoots than those treated with TDZ. The micro plantlets treated with BAP and TDZ in turn generated more shoots than the non-treated micro plantlets. In the test, the effect of concentration of BAP and TDZ in the medium was also evaluated; however not statistically significant differences between the tested concentrations were detected. In summary, findings indicate that the treatment with cytokines, BAP specifically, increases the number of shoots produced by micro plantlets J. neotropica. It is believed that this treatment can be applied on projects of mass propagation of this species within the frame of reforestation programs.