Browsing by Author "Rivera Pirela, Sergio Emiro"

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    Determinación de la diversidad genética y caracterización de genes de patogenicidad en aislados de Escherichia coli en pollos de engorde (Gallus gallus domesticus), Azuay, Ecuador
    (2023) Astudillo Riera, Fabián Manuel; Vallecillo Maza, Antonio Javier; Perez Pintado, Ana Cecilia; Rivera Pirela, Sergio Emiro
    In the poultry industry there are different types of pathogenic and non-pathogenic Escherichia coli, depending on the virulence associated genes (VAG) present in the bacteria. However, there are no reports for the area on the identification of E. coli VAG in broiler chickens especially in Ecuadorian andean communities. The main objective of this study was to identify the genetic diversity and the presence of VAG in E. coli strains. In this study, E. coli VAG were characterized in the Azuay Province (Ecuador) using isolates from 30 bacteriological cultures from chickens with clinical signs of colibacillosis. The PCR technique was used to detect the uspA gene (specific from E. coli) and also the GAV of adhesins (fimC), exotoxins (cvaA) and iron uptake-transport systems (iucD; chuA; fyuA) in each bacteria isolate. Pathogenic E. coli were molecularly typed by evaluating polytrinucleotide (GTG)5 sequences. The 83.33 % of the cultures presented the uspA gene from E. coli. The frequencies of positive VAG were 48 % for the chuA gene, 20 % for the cvaA gene, 84 % for the fimC gene, 36 % for the fyuA gene and 56 % for the iucD gene. Sequence evaluation (GTG)5 revealed two main phylogenetic groups of E. coli most of which carried at least one VAG gene. These results contribute to a more precise diagnosis of colibacillosis, as well as to is control and treatment by poultry farmers.
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    Eficacia de la prueba Elisa en muestras de leche para la vigilancia epidemiológica de la brucelosis bovina
    (Archivos Latinoamericanos de Producción Animal, 2022) Dután Sanango, Jorge Bolívar; Lupercio Novillo, Rosa Lucía; Andrade Guzmán, Omar Santiago; López Espinoza, Mateo Damián; Vintimilla Rojas, Andrea Elizabeth; Rivera Pirela, Sergio Emiro; Bustamante Ordóñez, Jorge Gualberto; Guevara Viera, Guillermo Null; Andrade Guzmán, Omar Santiago
    Bovine brucellosis is a worldwide zoonotic disease capable of infecting cattle for long periods of time. Some animals are asymptomatic, thus maintaining the disease in the herd. Cattle become infected after ingestion of contaminated milk, feed, water; also by contact with infected animals, uterine secretions or aborted fetuses and by vertical transmission. Little is known about the epidemiological situation of brucellosis in dairy herds in the province of Azuay. An initial step for the formulation of control and eradication programs would be the implementation of a surveillance system that allows characterizing the state of infection in dairy areas, and thus early detection of the entry of the disease into free herds. The purpose of this study was to evaluate the efficacy of the indirect ELISA test (IDEXX, Brucella tank milk) in milk pools as a tool to identify herds that have not been in contact with the disease, allowing the characterization of areas free of brucellosis.