Browsing by Author "Ortiz Molina, Paola Beatriz"

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Estudio de Toxoplasma gondii en suero sanguíneo de ganado bovino en tres centros de faenamiento de la provincia del Azuay mediante kit Elisa
(Universidad de Cuenca, 2021-07-21) Aguilar Valarezo, Fabián Enrique; Ortiz Molina, Paola Beatriz; Ramónez Cárdenas, Juan Carlos
Toxoplasmosis is a zoonosis with a wide worldwide distribution, caused by T. gondii. In Ecuador there is little information on the seroprevalence of T. gondii in cattle, while in other countries they report values between 10% to 40% in cattle and 30% to 90% in humans. The objective of this study was to determine the frequency of seropositive cattle to T. gondii prior to the sacrifice in the slaughter centers of the province of Azuay using the ELISA test and identifying if age, sex and origin are risk factors associated with the presence of T. gondii. We used 147 samples of blood serum, which were taken in the beds of Azuay certified by AGROCALIDAD, 76 from Gualaceo, 51 from Paute and 20 from Girón. The samples were processed in the laboratory for their analysis using the IDEXX Toxotest kit - ELISA test which determined there are 32.7% of seropositive animals (48/147) and 67.3% negative (99/147). When we applied the Chi-square test, there were not found significant values of variables studied (p>0.05), but a significant association was considered to the variable sex (OR=1.252) as a possible risk factor for the presence of the disease, being the males more prone to contracting the infection.
Presencia de Toxoplasma Gondii en suero sanguíneo de ganado bovino en empresas de rastro
(2024) Ortiz Molina, Paola Beatriz; Ramónez Cárdenas, Juan Carlos; Aguilar Valarezo, Fabián Enrique; Andrade Guzmán, Omar Santiago; Picón Saavedra, Marco Antonio; Haro Haro , Andrés Norberto; Vintimilla Rojas, Andrea Elizabeth
Infectious diseases such as toxoplasmosis develop by sexual replication in mammals, cattle and humans. In cattle it can cause abortions, fever, low feed intake, respiratory pathologies and nervous system disorders. The objective of this study was to analyze the frequency of bovines seropositive to Toxoplasma gondii before slaughter, in slaughterhouses in the Province of Azuay-Ecuador. From three areas, 148 animals were sampled and antibodies were identified using the ELISA technique. The study showed 48 seropositive cattle and a relative frequency of 33% of animals seroreactive Toxoplasma gondii. Together, a positive frequency of carrying the disease was observed among groups of cattle under and over 24 months of age. The frequency of carrying the disease by region before slaughter shows a 35% relative frequency in Giron slaughterhouse, defined as the area with the highest incidence of infected animals and carriers of the disease. Among the groups of cattle slaughtered, the seropositive frequency increases due to differences in gender and Region, therefore, toxoplasmosis is considered an invasive disease Worldwide and easily transmitted to humans by animals.